Assessing T Lymphocyte Aging Using Telomere Length and Telomerase Activity Measurements in Low Cell Numbers

2019 ◽  
pp. 231-243
Author(s):  
Tinhinane Fali ◽  
Camille K’Ros ◽  
Victor Appay ◽  
Delphine Sauce
Keyword(s):  
Telomere Length ◽  
T Lymphocyte ◽  
Telomerase Activity ◽  
Cell Numbers ◽  
Activity Measurements

33 TELOMERASE ACTIVITIES IN CLONED BEAGLE DOGS

2015 ◽  
Vol 27 (1) ◽  
pp. 109
Author(s):  
G. A. Kim ◽  
H. J. Oh ◽  
M. J. Kim ◽  
Y. K. Jo ◽  
E. M. N. Setyawan ◽  
...  
Keyword(s):  
Telomere Length ◽  
Donor Cell ◽  
Telomerase Activity ◽  
Physiological Age ◽  
Biological Aging ◽  
Amplification Protocol ◽  
Donor Cells ◽  
Activity Measurements ◽  
Natural Breeding

Telomerase is important ribonucleoprotein for restoring telomere length from its own RNA template. Regarding cloned animals derived from somatic cell nuclear transfer (SCNT), interesting questions have been raised about whether the cloning process restores cellular telomerase activity undergone by their donor cells. The present study was conducted to determine the effects of cloning on telomerase activity in the dog and normality of telomerase activity in cloned dogs. Focusing our attention on differences in telomerase activity depending on the age, we analysed telomerase activity in dogs produced by natural breeding of various ages. Comparison of the telomerase activities of cloned dogs and those of dogs produced by natural breeding was also performed. For SCNT, 2 cell donors, 7- and 9-year-old beagles, were used and donor cells were isolated from ear skin. After establishing donor cell lines, the enucleated canine in vivo-matured oocytes and the cells were injected and fused by electrofusion. After 30 days from embryo transfer, pregnancy diagnosis was performed and 7 cloned dogs were produced on the due date. For standardization of telomerase activity in beagles produced by natural breeding, blood of total 14 dogs at each age (10 months, 20 months, 5, 7, and 8 years old) were collected and telomerase activity was measured by the telomeric repeat amplification protocol (TRAP) assay. Telomerase activity measurements of at least 6 replications in each dog were performed. For statistical analysis, one-way ANOVA with Dunn's Multiple Comparison Test was used. Significant differences in telomerase activity were observed between the blood of cloned and donor dogs. It was shown that mean telomerase activities were decreased according to biological aging with significances. Mean telomerase activities in 10 cloned dogs were higher than those of a donor dog. Cloned dogs also showed similar levels of telomerase activities as their age-matched natural bred dogs, suggesting that they are within the variation in normal dogs. These observations indicate that the cloning process restores the telomerase activity in the dog. Thus, complex regulation of telomerase activity during nuclear reprogramming may regulate and be involved in telomerase activity in cloned dogs. It remains to be determined whether telomere length is correlated with telomerase activity and if it accurately reflects the physiological age of cloned dogs.This study was supported by IPET (#311062–04–2-SB010), RDA (PJ008975022013), Research Institute for Veterinary Science, the BK21 program, Nestle Purina Korea, and TS Corporation.

scholarly journals New Insights into Lymphocyte Differentiation and Aging from Telomere Length and Telomerase Activity Measurements

2019 ◽  
Vol 202 (7) ◽  
pp. 1962-1969 ◽  
Author(s):  
Tinhinane Fali ◽  
Laura Papagno ◽  
Charles Bayard ◽  
Yanis Mouloud ◽  
Jacques Boddaert ◽  
...  
Keyword(s):  
Telomere Length ◽  
Telomerase Activity ◽  
Lymphocyte Differentiation ◽  
Activity Measurements

Changes in Telomerase Activity and Telomere Length during Human T Lymphocyte Senescence

1997 ◽  
Vol 231 (2) ◽  
pp. 346-353 ◽  
Author(s):  
Chongxian Pan ◽  
Bao-Hua Xue ◽  
Thomas M. Ellis ◽  
David J. Peace ◽  
Manuel O. Dı́az
Keyword(s):  
Telomere Length ◽  
T Lymphocyte ◽  
Telomerase Activity ◽  
Human T Lymphocyte

638: Clinical relevance of telomere length and telomerase activity in colorectal cancer

2014 ◽  
Vol 50 ◽  
pp. S152
Author(s):  
C. De Juan Chocano ◽  
T. Fernández-Marcelo ◽  
I. Pascua ◽  
J. Head ◽  
A. Sánchez-Pernaute ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Telomere Length ◽  
Clinical Relevance ◽  
Telomerase Activity

Telomere length and telomerase activity: effect of ageing on human NK cells

2003 ◽  
Vol 124 (4) ◽  
pp. 403-408 ◽  
Author(s):  
Erminia Mariani ◽  
Alessandra Meneghetti ◽  
Ivan Formentini ◽  
Simona Neri ◽  
Luca Cattini ◽  
...  
Keyword(s):  
Nk Cells ◽  
Telomere Length ◽  
Telomerase Activity ◽  
Activity Effect

Expression of Telomerase RNA, Telomerase Activity, and Telomere Length in Human Gliomas

1997 ◽  
Vol 239 (3) ◽  
pp. 830-834 ◽  
Author(s):  
Ken Morii ◽  
Ryuichi Tanaka ◽  
Kiyoshi Onda ◽  
Itaru Tsumanuma ◽  
Jyunichi Yoshimura
Keyword(s):  
Telomere Length ◽  
Telomerase Activity ◽  
Telomerase Rna ◽  
Human Gliomas

scholarly journals Correlation of Telomere Length and Telomerase Activity with Occult Ovarian Insufficiency

2009 ◽  
Vol 23 (11) ◽  
pp. 1935-1935
Author(s):  
Samantha Butts ◽  
Harold Riethman ◽  
Sarah Ratcliffe ◽  
Alka Shaunik ◽  
Christos Coutifaris ◽  
...  
Keyword(s):  
Telomere Length ◽  
Telomerase Activity ◽  
Ovarian Insufficiency
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