Advanced Establishment of Stable Recombinant Human Suspension Cell Lines Using Genotype–Phenotype Coupling Transposon Vectors

2019 ◽  
pp. 351-361
Author(s):  
Karen Berg ◽  
Vanessa Nicole Schäfer ◽  
Natalie Tschorn ◽  
Jörn Stitz
Keyword(s):  
Cell Lines ◽  
Suspension Cell

scholarly journals Gene expression variability between randomly and targeted transgene integration events in tobacco suspension cell lines

2020 ◽  
Vol 14 (4) ◽  
pp. 451-458
Author(s):  
Janina Kirchhoff ◽  
Andreas Schiermeyer ◽  
Katja Schneider ◽  
Rainer Fischer ◽  
W. Michael Ainley ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Lines ◽  
Suspension Cell ◽  
Expression Variability ◽  
Transgene Integration ◽  
Tobacco Suspension Cell

Obtaining and Characteristics of Plant Cell Cultures of Аjuga turkestanica (Rgl.) - Producing of Phytoexdystroides

2019 ◽  
pp. 50-56
Author(s):  
G.I. Sobolkova ◽  
T.D. Kharitonov ◽  
A.M. Nigmatullaev ◽  
Sh.Sh. Sagdullaev ◽  
D.V. Kochkin ◽  
...  
Keyword(s):  
Cell Lines ◽  
Plant Cell ◽  
Cell Cultures ◽  
Plant Cell Cultures ◽  
Suspension Cell ◽  
Wild Plant ◽  
Russian Science ◽  
Growth Cycles ◽  
Ajuga Turkestanica

Callus cultures of Ajuga turkestanica (Rgl.) Briq. (Lamiaceae) were obtained from a wild plant, and the processes of morphogenesis in these cultures were studied. Depending on the hormonal composition of media, it is possible to obtain gemmogenesis or rhizogenesis in the first 6 to 8 growth cycles; after 10-15 growth cycles, the ability of morphogenesis was lost. Suspension cell cultures were initiated from some well-growing calluses. As a result, about one hundred calluses and suspension cell lines were obtained from the Ajuga turkestanica plant cells, a number of which were characterized by intensive growth and were analyzed by the HPLC-MS method for the presence of phytoecdysteroids. In most of the investigated lines, 20-hydroxyecdysone and turkesterone were found, the content of the first being 30-50 times higher than the second (in the most productive lines, 2.0-2.5 mg/g and 0.04-0.05 mg/g dry weight, respectively). An increase in the content of phytoecdysteroids in the in vitro cultivated cells by the end of the growing cycle was observed. However, phytoecdysteroids were not found in many of the obtained cell lines. Further research is needed to clarify the reasons for the presence or absence of phytoecdysteroids in Ajuga turkestanica plant cell cultures. Ajuga turkestanica, plant cell culture, growth regulators, differentiation, gemmogenesis, rhizogenesis, phytoecdysteroids, 20-hydroxy ecdysone, turkesterone The work was financially supported by the Russian Science Foundation (№ 16-14-00126).

Exploring the design space of AAV transient-transfection in suspension cell lines

2021 ◽  
pp. 341-360
Author(s):  
Oliver Meade ◽  
Jeffrey Clark ◽  
Michael McCutchen ◽  
John Kerwin
Keyword(s):  
Cell Lines ◽  
Design Space ◽  
Transient Transfection ◽  
Suspension Cell

scholarly journals Investigation of Heavy Metal Hyperaccumulation at the Cellular Level: Development and Characterization of Thlaspi caerulescens Suspension Cell Lines

2008 ◽  
Vol 147 (4) ◽  
pp. 2006-2016 ◽  
Author(s):  
Melinda A. Klein ◽  
Hitoshi Sekimoto ◽  
Matthew J. Milner ◽  
Leon V. Kochian
Keyword(s):  
Heavy Metal ◽  
Cell Lines ◽  
Cellular Level ◽  
Thlaspi Caerulescens ◽  
Suspension Cell ◽  
Metal Hyperaccumulation

A Robust Protocol for CRISPR‐Cas9 Gene Editing in Human Suspension Cell Lines

2021 ◽  
Vol 1 (11) ◽  
Author(s):  
Joanna D. Wardyn ◽  
Allison S. Y. Chan ◽  
Anand D. Jeyasekharan
Keyword(s):  
Cell Lines ◽  
Gene Editing ◽  
Suspension Cell ◽  
Robust Protocol

New avian suspension cell lines provide production of influenza virus and MVA in serum-free media: Studies on growth, metabolism and virus propagation

Vaccine ◽  
2009 ◽  
Vol 27 (36) ◽  
pp. 4975-4982 ◽  
Author(s):  
V. Lohr ◽  
A. Rath ◽  
Y. Genzel ◽  
I. Jordan ◽  
V. Sandig ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Media Studies ◽  
Cell Lines ◽  
Suspension Cell ◽  
Virus Propagation ◽  
Serum Free ◽  
Growth Metabolism ◽  
Free Media

A Novel Scalable Production Platform for Gene Therapy Vectors Based on Human Suspension Cell Lines

2017 ◽  
Vol 15 (4) ◽  
pp. 8-15
Author(s):  
Nicole Faust ◽  
◽  
Kerstin Hein ◽  
Simon Fradin ◽  
Helmut Kewes ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Cell Lines ◽  
Suspension Cell ◽  
Production Platform ◽  
Gene Therapy Vectors ◽  
Scalable Production

scholarly journals Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell lines

2022 ◽  
Vol 3 (1) ◽  
pp. 101037
Author(s):  
Cai Han ◽  
Linyu Sun ◽  
Qi Pan ◽  
Yumeng Sun ◽  
Wentao Wang ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Cell Lines ◽  
Suspension Cell ◽  
Polysome Profiling ◽  
Non Coding Rnas

scholarly journals CELL STORAGE-02-Freezing and Thawing Protocol for Suspension Cell Lines v1

protocols.io ◽  
2021 ◽  
Author(s):  
Marco Cosentino ◽  
Alessandra Luini ◽  
Massimiliano Legnaro ◽  
Emanuela Rasini ◽  
Mariagiulia Albizzati ◽  
...  
Keyword(s):  
Cell Lines ◽  
Suspension Cell ◽  
Freezing And Thawing ◽  
Cell Storage

scholarly journals Development and application of a cultivation platform for mammalian suspension cell lines with single‐cell resolution

2020 ◽  
Author(s):  
Julian Schmitz ◽  
Sarah Täuber ◽  
Christoph Westerwalbesloh ◽  
Eric Lieres ◽  
Thomas Noll ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Lines ◽  
Suspension Cell
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