Transient Transformation of Plants

2009 ◽  
pp. 131-152 ◽  
Author(s):  
Huw D. Jones ◽  
Angela Doherty ◽  
Caroline A. Sparks
Keyword(s):  
Transient Transformation

An efficient transient transformation system for gene function studies in pumpkin (Cucurbita moschata D.)

2021 ◽  
Vol 282 ◽  
pp. 110028
Author(s):  
Xuejin Chen ◽  
Songtao He ◽  
Lina Jiang ◽  
Xinzheng Li ◽  
Weili Guo ◽  
...  
Keyword(s):  
Gene Function ◽  
Transient Transformation ◽  
Cucurbita Moschata ◽  
Transformation System

scholarly journals Agrobacterium-mediated transient transformation of sorghum leaves for accelerating functional genomics and genome editing studies

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Rita Sharma ◽  
Yan Liang ◽  
Mi Yeon Lee ◽  
Venkataramana R. Pidatala ◽  
Jenny C. Mortimer ◽  
...  
Keyword(s):  
Functional Genomics ◽  
Genome Editing ◽  
Transient Transformation

scholarly journals Illuminating the cells: transient transformation of citrus to study gene functions and organelle activities related to fruit quality

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Jinli Gong ◽  
Zhen Tian ◽  
Xiaolu Qu ◽  
Qiunan Meng ◽  
Yajie Guan ◽  
...  
Keyword(s):  
Membrane Trafficking ◽  
Protein Localization ◽  
Cell Physiology ◽  
Transient Transformation ◽  
Citrus Fruits ◽  
Efficient System ◽  
Fluorescent Reporters ◽  
Biology Research ◽  
Insight Into ◽  
Microscopic Techniques

AbstractAlthough multiple microscopic techniques have been applied to horticultural research, few studies of individual organelles in living fruit cells have been reported to date. In this paper, we established an efficient system for the transient transformation of citrus fruits using an Agrobacterium-mediated method. Kumquat (Fortunella crassifolia Swingle) was used; it exhibits higher transformation efficiency than all citrus fruits that have been tested and a prolonged-expression window. Fruits were transformed with fluorescent reporters, and confocal microscopy and live-cell imaging were used to study their localization and dynamics. Moreover, various pH sensors targeting different subcellular compartments were expressed, and the local pH environments in cells from different plant tissues were compared. The results indicated that vacuoles are most likely the main organelles that contribute to the low pH of citrus fruits. In summary, our method is effective for studying various membrane trafficking events, protein localization, and cell physiology in fruit and can provide new insight into fruit biology research.

scholarly journals <i>Agrobacterium</i>-mediated transient transformation of <i>Pentalinon andrieuxii</i> Müll. Arg.

2012 ◽  
Vol 03 (03) ◽  
pp. 256-258 ◽  
Author(s):  
Alejandro Yam-Puc ◽  
Elidé Avilés-Berzunza ◽  
Manuel J. Chan-Bacab ◽  
Luis Manuel Peña-Rodríguez ◽  
Gregorio Godoy-Hernández
Keyword(s):  
Transient Transformation

scholarly journals An improved and efficient method of Agrobacterium syringe infiltration for transient transformation and its application in the elucidation of gene function in poplar

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Lin Zheng ◽  
Jixiu Yang ◽  
Yajuan Chen ◽  
Liping Ding ◽  
Jianhua Wei ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Secondary Wall ◽  
Functional Characterization ◽  
Bacterial Suspension ◽  
Wall Thickening ◽  
Vessel Element ◽  
Transient Transformation ◽  
Transformed Plants ◽  
Poplar Clone

Abstract Background Forest trees have important economic and ecological value. As a model tree, poplar has played a significant role in elucidating the molecular mechanisms underlying tree biology. However, a lack of mutant libraries and time-consuming stable genetic transformation processes severely limit progress into the functional characterization of poplar genes. A convenient and fast transient transformation method is therefore needed to enhance progress on functional genomics in poplar. Methods A total of 11 poplar clones were screened for amenability to syringe infiltration. Syringe infiltration was performed on the lower side of the leaves of young soil-grown plants. Transient expression was evaluated by visualizing the reporters β-glucuronidase (GUS) and green fluorescent protein (GFP). The experimental parameters of the syringe agroinfiltration were optimized based on the expression levels of the reporter luciferase (LUC). Stably transformed plants were regenerated from transiently transformed leaf explants through callus-induced organogenesis. The functions of Populus genes in secondary cell wall-thickening were characterized by visualizing lignin deposition therein after staining with basic fuchsin. Results We greatly improved the transient transformation efficiency of syringe Agrobacterium infiltration in poplar through screening for a suitable poplar clone from a variety of clones and optimizing the syringe infiltration procedure. The selected poplar clone, Populus davidiana × P. bolleana, is amenable to Agrobacterium syringe infiltration, as indicated by the easy diffusion of the bacterial suspension inside the leaf tissues. Using this technique, we localized a variety of poplar proteins in specific intracellular organelles and illustrated the protein–protein and protein–DNA interactions. The transiently transformed leaves could be used to generate stably transformed plants with high efficiency through callus induction and differentiation processes. Furthermore, transdifferentiation of the protoxylem-like vessel element and ectopic secondary wall thickening were induced in the agroinfiltrated leaves via the transient overexpression of genes associated with secondary wall formation. Conclusions The application of P. davidiana × P. bolleana in Agrobacterium syringe infiltration provides a foundation for the rapid and high-throughput functional characterization of Populus genes in intact poplar plants, including those involved in wood formation, and provides an effective alternative to Populus stable genetic transformation.

Dissection of a pollen-specific promoter from maize by transient transformation assays

1992 ◽  
Vol 18 (2) ◽  
pp. 211-218 ◽  
Author(s):  
Douglas A. Hamilton ◽  
Mihir Roy ◽  
Julia Rueda ◽  
Ram K. Sindhu ◽  
John Sanford ◽  
...  
Keyword(s):  
Transient Transformation

Agrobacterium-mediated transient transformation of Bienertia sinuspersici to assay recombinant protein distribution between dimorphic chloroplasts

2019 ◽  
Vol 38 (7) ◽  
pp. 779-782 ◽  
Author(s):  
Diana Wimmer ◽  
Philipp Bohnhorst ◽  
Daniela Impe ◽  
Inhwan Hwang ◽  
Sascha Offermann
Keyword(s):  
Recombinant Protein ◽  
Transient Transformation ◽  
Protein Distribution ◽  
Bienertia Sinuspersici

Development of a Particle Bombardment-Mediated Transient Transformation System for Taxus spp. Cells in Culture

2007 ◽  
Vol 0 (0) ◽  
pp. 0-0 ◽  
Author(s):  
K. Vongpaseuth ◽  
E. Nims ◽  
M. St.Amand ◽  
E.L. Walker ◽  
S.C. Roberts
Keyword(s):  
Particle Bombardment ◽  
Transient Transformation ◽  
Transformation System ◽  
Cells In Culture
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