Sperm Cryopreservation and In Vitro Fertilization

2009 ◽  
pp. 407-420 ◽  
Author(s):  
Susan Marschall ◽  
Auke Boersma ◽  
Martin Hrabé de Angelis
Keyword(s):  
In Vitro Fertilization ◽  
Sperm Cryopreservation ◽  
Vitro Fertilization

Effect of sperm cryopreservation and treatment with calcium ionophore or heparin on in vitro fertilization of horse oocytes

2001 ◽  
Vol 56 (5) ◽  
pp. 817-829 ◽  
Author(s):  
H Alm ◽  
H Torner ◽  
S Blottner ◽  
G Nürnberg ◽  
W Kanitz
Keyword(s):  
In Vitro Fertilization ◽  
Calcium Ionophore ◽  
Sperm Cryopreservation ◽  
Vitro Fertilization

Sperm cryopreservation, in vitro fertilization, and embryo freezing

2022 ◽  
pp. 157-181
Author(s):  
Kanav Khosla ◽  
John Bischof ◽  
Zoltán M. Varga
Keyword(s):  
In Vitro Fertilization ◽  
Sperm Cryopreservation ◽  
Vitro Fertilization ◽  
Embryo Freezing

scholarly journals In Vitro Fertilization and Sperm Cryopreservation in the Black-Footed Cat (Felis nigripes) and Sand Cat (Felis margarita)1

2010 ◽  
Vol 82 (3) ◽  
pp. 552-562 ◽  
Author(s):  
J.R. Herrick ◽  
M. Campbell ◽  
G. Levens ◽  
T. Moore ◽  
K. Benson ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Sperm Cryopreservation ◽  
Vitro Fertilization ◽  
Sand Cat

A simple and economic protocol for efficient in vitro fertilization using cryopreserved mouse sperm v2

2021 ◽  
Author(s):  
Magdalena Wigger ◽  
Simon E Tröder ◽  
Branko Zevnik
Keyword(s):  
In Vitro Fertilization ◽  
Mouse Models ◽  
Cost Effective ◽  
Genetically Engineered ◽  
Sperm Cryopreservation ◽  
Vitro Fertilization ◽  
Genetically Engineered Mouse Models ◽  
Embryo Freezing ◽  
Cryopreserved Sperm

The advent of genome editing tools like CRISPR/Cas has substantially increased the number of genetically engineered mouse models in recent years. In support of refinement and reduction, sperm cryopreservation is advantageous compared to embryo freezing for archiving and distribution of such mouse models. The in vitro fertilization using cryopreserved sperm from the most widely used C57BL/6 strain has become highly efficient in recent years due to several improvements of the procedure. However, purchase of the necessary media for routine application of the current protocol poses a constant burden on budgetary constraints. In-house media preparation, instead, is complex and requires quality control of each batch. Here, we describe a cost-effective and easily adaptable approach for in vitro fertilization using cryopreserved C57BL/6 sperm. This is mainly achieved by modification of an affordable commercial fertilization medium and a step-by-step description of all other necessary reagents. This protocol is compatible with frozen sperm from all major repositories and the IVF can easily be adapted to accommodate freshly harvested sperm.

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