Xenopus Oocytes as a Heterologous Expression System for Studying Ion Channels with the Patch-Clamp Technique

2008 ◽  
pp. 127-139 ◽  
Author(s):  
Paolo Tammaro ◽  
Kenju Shimomura ◽  
Peter Proks
Keyword(s):  
Ion Channels ◽  
Patch Clamp ◽  
Heterologous Expression ◽  
Xenopus Oocytes ◽  
Expression System ◽  
Heterologous Expression System ◽  
Patch Clamp Technique ◽  
Clamp Technique

Patch-clamp technique in renal physiology

1986 ◽  
Vol 250 (3) ◽  
pp. F379-F385 ◽  
Author(s):  
L. G. Palmer
Keyword(s):  
Ion Channels ◽  
Patch Clamp ◽  
Ion Transport ◽  
Renal Physiology ◽  
Patch Clamp Technique ◽  
Clamp Technique ◽  
Basic Concepts

The patch-clamp technique of Neher and Sakmann and their colleagues has been widely used over the last 5 years to investigate ion channels in excitable tissues. More recently, it has become useful as a tool to study channels involved in transepithelial ion transport. In this review, I briefly cover the basic concepts behind the patch-clamp technique and the kinds of information that can be obtained with it. I then summarize the applications of the technique to renal tissues and describe some of the channel types that have been observed to date in epithelia.

Electrophysiological investigation of anthelmintic resistance

Parasitology ◽  
2000 ◽  
Vol 120 (7) ◽  
pp. 87-94 ◽  
Author(s):  
R. J. MARTIN ◽  
A. P. ROBERTSON
Keyword(s):  
Ion Channels ◽  
Patch Clamp ◽  
Anthelmintic Resistance ◽  
Resistant Isolate ◽  
Patch Clamp Technique ◽  
Clamp Technique ◽  
Receptor Sites ◽  
Flow Through ◽  
Sites Of Action ◽  
A Site

It is pointed out that two of the three major groups of anthelmintic act by opening membrane ion-channels. It is appropriate, therefore, to use electrophysiological methods to study the properties of the sites of action of these drugs and the changes in the properties of these receptor sites associated with resistance. This paper describes the use of the patch- clamp technique to observe the currents that flow through the levamisole-activated channels as they open and close in levamisole-sensitive and levamisole-resistant isolates. It was found that, on average, the proportion of time the channels are open, is less in the resistant isolate. The patch-clamp technique also showed that the ion-channels are heterogeneous and that one of the subtypes is lost with the appearance of resistance. The use of the current clamp technique is illustrated to record a site of action of ivermectin in the pharyngeal muscle of Ascaris.

Patch-clamp technique to characterize ion channels in enlarged individual endolysosomes

2017 ◽  
Vol 12 (8) ◽  
pp. 1639-1658 ◽  
Author(s):  
Cheng-Chang Chen ◽  
Chunlei Cang ◽  
Stefanie Fenske ◽  
Elisabeth Butz ◽  
Yu-Kai Chao ◽  
...  
Keyword(s):  
Ion Channels ◽  
Patch Clamp ◽  
Patch Clamp Technique ◽  
Clamp Technique

Patch-Clamp Recording of Ion Channels: Interfering Effects of Patch Pipette Glass

Physiology ◽  
1990 ◽  
Vol 5 (4) ◽  
pp. 155-158
Author(s):  
C Zuazaga ◽  
A Steinacker
Keyword(s):  
Ion Channels ◽  
Patch Clamp ◽  
Ion Channel ◽  
Channel Activity ◽  
Patch Clamp Recording ◽  
Patch Clamp Technique ◽  
Clamp Technique ◽  
Ion Channel Properties ◽  
Patch Pipette ◽  
Channel Properties

Glass for pipettes used to record ion channel activity with the patch-clamp technique is selected on the basis of its electrical, thermal, and sealing properties. Recent findings stress a new characteristic to consider: the effect of pipette glass itself on ion channel properties.

scholarly journals Xenopus oocytes as a heterologous expression system for analysis of tight junction proteins

2019 ◽  
Vol 33 (4) ◽  
pp. 5312-5319 ◽  
Author(s):  
Constanze Vitzthum ◽  
Laura Stein ◽  
Nora Brunner ◽  
Ria Knittel ◽  
Petra Fallier‐Becker ◽  
...  
Keyword(s):  
Tight Junction ◽  
Heterologous Expression ◽  
Xenopus Oocytes ◽  
Expression System ◽  
Tight Junction Proteins ◽  
Heterologous Expression System ◽  
Junction Proteins

Shock Wave Therapy as an Innovative Technology in Skeletal Disorders: Study on Transmembrane Current in Stimulated Osteoblast-Like Cells

2005 ◽  
Vol 28 (8) ◽  
pp. 841-847 ◽  
Author(s):  
L. Martini ◽  
G. Giavaresi ◽  
M. Fini ◽  
P. Torricelli ◽  
V. Borsari ◽  
...  
Keyword(s):  
Shock Wave ◽  
Ion Channels ◽  
Patch Clamp ◽  
Ionic Current ◽  
Innovative Technology ◽  
Patch Clamp Recording ◽  
Patch Clamp Technique ◽  
Transmembrane Current ◽  
Clamp Technique ◽  
And Control

Extracorporeal shock wave treatment (ESWT) is successfully used in various musculoskeletal disorders and pathologies. Despite the increasing use of this kind of therapy, some aspects of its mechanism of action are still unclear. In vitro bone cell behavior under ESWT were previously investigated by the present author and MG63 osteoblast-like cells showed an enhancement in proliferation and in the osteoblast differentiation after therapy with a low-energy flux density. The aim of the present study was to evaluate the effect of ESWT on the permeabilization of cell membrane. We characterized physiological changes in the MG63 associated with ESWT generated by an ESW device and patch clamp recording was performed to study ion channels. Experiments were carried out using the whole-cell recording configuration of the patch-clamp technique and the ionic current measurements were performed on cell samples of ESW treated and control groups. The patch-clamp technique showed the effect of ESWT on the amplitude of transmembrane currents. The treatment with ESW enhanced the transmembrane current as well the voltage dependence of Ca-activated and K channels that mediate these currents: the differences between treated cells and control at 80mV were over 1000 pA (P<0.05). These modifications of ion channels activity positively influence cell proliferation (MTT test, P<0.0001) without interfering with the normal synthesis activity of stimulated osteoblasts.

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