Cell Surface Markers in Human Embryonic Stem Cells

2007 ◽  
pp. 51-61 ◽  
Author(s):  
Raj R. Rao ◽  
Alison Venable Johnson ◽  
Steven L. Stice
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cell Surface ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Surface Markers ◽  
Cell Surface Markers

Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells

2011 ◽  
Vol 29 (8) ◽  
pp. 750-756 ◽  
Author(s):  
Olivia G Kelly ◽  
Man Yin Chan ◽  
Laura A Martinson ◽  
Kuniko Kadoya ◽  
Traci M Ostertag ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cell Surface ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Types ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Pancreatic Cell

scholarly journals The ROCK Inhibitor Y-27632 Improves Recovery of Human Embryonic Stem Cells after Fluorescence-Activated Cell Sorting with Multiple Cell Surface Markers

PLoS ONE ◽  
2010 ◽  
Vol 5 (8) ◽  
pp. e12148 ◽  
Author(s):  
Nil Emre ◽  
Jason G. Vidal ◽  
Jeanne Elia ◽  
Eric D. O'Connor ◽  
Rosanto I. Paramban ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cell Surface ◽  
Human Embryonic Stem Cells ◽  
Cell Sorting ◽  
Embryonic Stem ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Rock Inhibitor ◽  
Multiple Cell

Surface markers of human embryonic stem cells: a meta analysis of membrane proteomics reports

2018 ◽  
Vol 15 (11) ◽  
pp. 911-922 ◽  
Author(s):  
Faezeh Shekari ◽  
Chia-Li Han ◽  
Jaesuk Lee ◽  
Mehdi Mirzaei ◽  
Vivek Gupta ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Meta Analysis ◽  
Embryonic Stem ◽  
Surface Markers ◽  
Membrane Proteomics

304 THE DEVELOPMENTAL TOXICITY EVALUATION OF 5-FLUOROURACIL AND INDOMETHACIN IN HUMAN EMBRYONIC STEM CELLS

2013 ◽  
Vol 25 (1) ◽  
pp. 299
Author(s):  
E. M. Jung ◽  
E. B. Jeung
Keyword(s):  
Stem Cells ◽  
Transcription Factors ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Developmental Process ◽  
Developmental Toxicity ◽  
Embryonic Stem ◽  
Surface Markers ◽  
Dependent Manner ◽  
Undifferentiated Hesc

Embryonic stem cells have pluripotency and differentiate into and constitute the cells and tissues of our body. In this study, using human embryonic stem cells (hESC), we evaluated novel methods for screening toxicological chemicals during developmental process. We elucidated developmental toxicity of two well-known chemicals, 5-fluorouracil (5-FU) and indomethacin (Indo) in hESC. The undifferentiated hESC were treated with the chemicals (10–4 to 104 µM of 5-FU and Indo) in a dose-dependent manner during 1 to 3 days. Surface markers (SSEA-4, TRA-1-60, and TRA-1-81) expressed only in undifferentiated hESC were monitored by immunocytochemistry to ensure the characterisation of undifferentiated hESC. Moreover, expression of embryonic stem cell-specific genes was assessed with real-time PCR after treatment of 5-FU and Indo (10–2, 100, and102 µM of 5-FU and Indo). The expression of surface markers was not significantly affected by treatment of 5-FU and Indo. The expression of transcription factors (Oct-4, Sox-2, Nanog, and hTERT) was significantly decreased by high concentrations of 5-FU and Indo (102 µM). However, no difference was observed in treatment of low concentration of 5-FU and Indo (10–2 µM). Taken together, these results suggest that 5-FU and Indo have cytotoxic effects, and modulate the expression of transcription factors that have pivotal roles in undifferentiated hESC. Therefore, we suggest that hESC may have potential to test toxicity of chemicals during embryonic developmental stage.

scholarly journals Proteomic Analyses Reveal Common Promiscuous Patterns of Cell Surface Proteins on Human Embryonic Stem Cells and Sperms

PLoS ONE ◽  
2011 ◽  
Vol 6 (5) ◽  
pp. e19386 ◽  
Author(s):  
Bin Gu ◽  
Jiarong Zhang ◽  
Ying Wu ◽  
Xinzong Zhang ◽  
Zhou Tan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cell Surface ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Surface Proteins ◽  
Cell Surface Proteins ◽  
Proteomic Analyses

scholarly journals The Cell-Surface N-Glycome of Human Embryonic Stem Cells and Differentiated Hepatic Cells thereof

ChemBioChem ◽  
2017 ◽  
Vol 18 (13) ◽  
pp. 1234-1241 ◽  
Author(s):  
Houda Montacir ◽  
Nora Freyer ◽  
Fanny Knöspel ◽  
Thomas Urbaniak ◽  
Tereza Dedova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cell Surface ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Hepatic Cells
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