Single-Molecule Imaging Techniques to Visualize Chemotactic Signaling Events on the Membrane of Living Dictyostelium Cells

2009 ◽  
pp. 417-435 ◽  
Author(s):  
Yukihiro Miyanaga ◽  
Satomi Matsuoka ◽  
Masahiro Ueda
Keyword(s):  
Single Molecule ◽  
Imaging Techniques ◽  
Single Molecule Imaging ◽  
Signaling Events

Stochastic signal inputs for chemotactic response in Dictyostelium cells revealed by single molecule imaging techniques

Biosystems ◽  
2007 ◽  
Vol 88 (3) ◽  
pp. 251-260 ◽  
Author(s):  
Yukihiro Miyanaga ◽  
Satomi Matsuoka ◽  
Toshio Yanagida ◽  
Masahiro Ueda
Keyword(s):  
Single Molecule ◽  
Imaging Techniques ◽  
Chemotactic Response ◽  
Single Molecule Imaging ◽  
Stochastic Signal

scholarly journals Dynamics and Signal Transduction of EGFR Clusters Visualized by Single-molecule Imaging Techniques

2001 ◽  
Vol 41 (supplement) ◽  
pp. S119
Author(s):  
Y. Sako ◽  
S. Minoguchi ◽  
A. Yoshimura ◽  
T. Yanagida
Keyword(s):  
Signal Transduction ◽  
Single Molecule ◽  
Imaging Techniques ◽  
Single Molecule Imaging

Combining Single Molecule Imaging Techniques to Unravel the Nanoscale Organization of the Presynapse

2020 ◽  
pp. 265-286
Author(s):  
Christopher Small ◽  
Ramon Martínez-Mármol ◽  
Rumelo Amor ◽  
Frederic A. Meunier ◽  
Merja Joensuu
Keyword(s):  
Single Molecule ◽  
Imaging Techniques ◽  
Single Molecule Imaging

Single-Molecule Imaging of Stochastic Signaling Events in Living Cells

2012 ◽  
Vol 2012 (3) ◽  
pp. pdb.top068189-pdb.top068189
Author(s):  
S. Matsuoka ◽  
Y. Miyanaga ◽  
T. Yanagida ◽  
M. Ueda
Keyword(s):  
Single Molecule ◽  
Living Cells ◽  
Single Molecule Imaging ◽  
Signaling Events

scholarly journals Accurate EMCCD Photoelectron Calibration for Single Molecule Imaging Techniques

2013 ◽  
Vol 104 (2) ◽  
pp. 666a
Author(s):  
Alexander Song ◽  
Han Gao ◽  
Avtar Singh ◽  
Warren R. Zipfel
Keyword(s):  
Single Molecule ◽  
Imaging Techniques ◽  
Single Molecule Imaging

Label-free, mass-sensitive single-molecule imaging using interferometric scattering microscopy

2020 ◽  
Author(s):  
Nikolas Hundt
Keyword(s):  
Single Molecule ◽  
Cellular Systems ◽  
Single Molecule Imaging ◽  
Label Free ◽  
Measurement Sensitivity ◽  
Mass Distributions ◽  
Quantitative Measurements ◽  
Contrast Mechanism ◽  
Cellular Components ◽  
Scattering Signal

Abstract Single-molecule imaging has mostly been restricted to the use of fluorescence labelling as a contrast mechanism due to its superior ability to visualise molecules of interest on top of an overwhelming background of other molecules. Recently, interferometric scattering (iSCAT) microscopy has demonstrated the detection and imaging of single biomolecules based on light scattering without the need for fluorescent labels. Significant improvements in measurement sensitivity combined with a dependence of scattering signal on object size have led to the development of mass photometry, a technique that measures the mass of individual molecules and thereby determines mass distributions of biomolecule samples in solution. The experimental simplicity of mass photometry makes it a powerful tool to analyse biomolecular equilibria quantitatively with low sample consumption within minutes. When used for label-free imaging of reconstituted or cellular systems, the strict size-dependence of the iSCAT signal enables quantitative measurements of processes at size scales reaching from single-molecule observations during complex assembly up to mesoscopic dynamics of cellular components and extracellular protrusions. In this review, I would like to introduce the principles of this emerging imaging technology and discuss examples that show how mass-sensitive iSCAT can be used as a strong complement to other routine techniques in biochemistry.

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