Monitoring Dynamic GPCR Signaling Events Using Fluorescence Microscopy, FRET Imaging, and Single-Molecule Imaging

2009 ◽  
pp. 371-383 ◽  
Author(s):  
Xuehua Xu ◽  
Joseph A. Brzostowski ◽  
Tian Jin
Keyword(s):  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Single Molecule Imaging ◽  
Gpcr Signaling ◽  
Signaling Events

scholarly journals Assessing low-light cameras with photon transfer curve method

2016 ◽  
Vol 09 (03) ◽  
pp. 1630008 ◽  
Author(s):  
Luchang Li ◽  
Mengting Li ◽  
Zhaoning Zhang ◽  
Zhen-Li Huang
Keyword(s):  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Single Molecule Imaging ◽  
Specific Technique ◽  
Low Light ◽  
New Type ◽  
Curve Method ◽  
Microscopy Techniques ◽  
Noise Map

Low-light camera is an indispensable component in various fluorescence microscopy techniques. However, choosing an appropriate low-light camera for a specific technique (for example, single molecule imaging) is always time-consuming and sometimes confusing, especially after the commercialization of a new type of camera called sCMOS camera, which is now receiving heavy demands and high praise from both academic and industrial users. In this tutorial, we try to provide a guide on how to fully access the performance of low-light cameras using a well-developed method called photon transfer curve (PTC). We first present a brief explanation on the key parameters for characterizing low-light cameras, then explain the experimental procedures on how to measure PTC. We also show the application of the PTC method in experimentally quantifying the performance of two representative low-light cameras. Finally, we extend the PTC method to provide offset map, read noise map, and gain map of individual pixels inside a camera.

Single-Molecule Imaging of Stochastic Signaling Events in Living Cells

2012 ◽  
Vol 2012 (3) ◽  
pp. pdb.top068189-pdb.top068189
Author(s):  
S. Matsuoka ◽  
Y. Miyanaga ◽  
T. Yanagida ◽  
M. Ueda
Keyword(s):  
Single Molecule ◽  
Living Cells ◽  
Single Molecule Imaging ◽  
Signaling Events

Fluorescence microscopy in superfluid helium: Single molecule imaging

1996 ◽  
Vol 67 (4) ◽  
pp. 1425-1430 ◽  
Author(s):  
Jan Jasny ◽  
Jerzy Sepiol ◽  
Thomas Irngartinger ◽  
Markus Traber ◽  
Alois Renn ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Single Molecule ◽  
Superfluid Helium ◽  
Single Molecule Imaging
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