Mammalian Membrane Protein Expression in Baculovirus-Infected Insect Cells

2009 ◽  
pp. 105-117 ◽  
Author(s):  
Céline Trometer ◽  
Pierre Falson
Keyword(s):  
Membrane Protein ◽  
Protein Expression ◽  
Insect Cells ◽  
Membrane Protein Expression ◽  
Infected Insect

Rapid screening of membrane protein expression in transiently transfected insect cells

2013 ◽  
Vol 88 (1) ◽  
pp. 134-142 ◽  
Author(s):  
Hao Chen ◽  
Paul L. Shaffer ◽  
Xin Huang ◽  
Paul E. Rose
Keyword(s):  
Membrane Protein ◽  
Protein Expression ◽  
Insect Cells ◽  
Rapid Screening ◽  
Membrane Protein Expression

Quantitative Membrane Protein Expression at the Blood–Brain Barrier of Adult and Younger Cynomolgus Monkeys

2011 ◽  
Vol 100 (9) ◽  
pp. 3939-3950 ◽  
Author(s):  
Katsuaki Ito ◽  
Yasuo Uchida ◽  
Sumio Ohtsuki ◽  
Sanshiro Aizawa ◽  
Hirotaka Kawakami ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Expression ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Cynomolgus Monkeys ◽  
Membrane Protein Expression ◽  
Blood Brain

scholarly journals Sex Hormones Regulate Rat Hepatic Monocarboxylate Transporter Expression and Membrane Trafficking

2017 ◽  
Vol 20 (1) ◽  
pp. 435 ◽  
Author(s):  
Jieyun Cao ◽  
Michael Ng ◽  
Melanie A Felmlee
Keyword(s):  
Sex Differences ◽  
Membrane Protein ◽  
Protein Expression ◽  
Estrous Cycle ◽  
Sex Hormones ◽  
Membrane Trafficking ◽  
Drug Disposition ◽  
Membrane Expression ◽  
Membrane Protein Expression ◽  
Estrous Cycle Stage

Purpose: Monocarboxylate transporters (MCTs) are involved in the transport of monocarboxylates such as ketone bodies, lactate, and pharmaceutical agents. CD147 functions as an ancillary protein for MCT1 and MCT4 for plasma membrane trafficking. Sex differences in MCT1 and MCT4 have been observed in muscle and reproductive tissues; however, there is a paucity of information on MCT sex differences in tissues involved in drug disposition. The objective of the present study was to quantify hepatic MCT1, MCT4 and CD147 mRNA, total cellular and membrane protein expression in males, over the estrous cycle in females and in ovariectomized (OVX) females. Method: Liver samples were collected from females at the four estrous cycle stages (proestrus, estrus, metestrus, diestrus), OVX females and male Sprague-Dawley rats (N = 3 – 5). Estrus cycle stage of females was determined by vaginal lavage. mRNA and protein (total and membrane) expression of MCT1, MCT4 and CD147 was evaluated by qPCR and western blot analysis. Results: MCT1 mRNA and membrane protein expression varied with estrous cycle stage, with OVX females having higher expression than males, indicating that female sex hormones may play a role in MCT1 regulation. MCT4 membrane expression varied with estrous cycle stage with expression significantly lower than males. MCT4 membrane expression in OVX females was also lower than males, suggesting that androgens play a role in membrane expression of MCT4. Males had higher membrane CD147 expression, whereas there was no difference in whole cell protein and mRNA levels suggesting that androgens are involved in regulating CD147 membrane localization. Conclusions: This study demonstrates hepatic expression and membrane localization of MCT1, MCT4 and CD147 are regulated by sex hormones. Sex differences in hepatic MCT expression may lead to altered drug disposition, so it is critical to elucidate the underlying mechanisms in the sex hormone-dependent regulation of MCT expression. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

scholarly journals Reprogramming chaperone pathways to improve membrane protein expression in Escherichia coli

2011 ◽  
Vol 20 (8) ◽  
pp. 1411-1420 ◽  
Author(s):  
Brent L. Nannenga ◽  
François Baneyx
Keyword(s):  
Escherichia Coli ◽  
Membrane Protein ◽  
Protein Expression ◽  
Expression In Escherichia Coli ◽  
Membrane Protein Expression

scholarly journals Integral Membrane Protein Expression in Saccharomyces cerevisiae

2016 ◽  
pp. 163-186
Author(s):  
Rebba C. Boswell-Casteel ◽  
Jennifer M. Johnson ◽  
Robert M. Stroud ◽  
Franklin A. Hays
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Membrane Protein ◽  
Protein Expression ◽  
Integral Membrane Protein ◽  
Membrane Protein Expression

scholarly journals Impact of hydroxycarbamide and interferon-α on red cell adhesion and membrane protein expression in polycythemia vera

Haematologica ◽  
2018 ◽  
Vol 103 (6) ◽  
pp. 972-981 ◽  
Author(s):  
Mégane Brusson ◽  
Maria De Grandis ◽  
Sylvie Cochet ◽  
Sylvain Bigot ◽  
Mickaël Marin ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Membrane Protein ◽  
Protein Expression ◽  
Polycythemia Vera ◽  
Interferon Α ◽  
Red Cell ◽  
Membrane Protein Expression

Membrane Protein Expression in Lactococcus lactis

2015 ◽  
pp. 77-97 ◽  
Author(s):  
Martin S. King ◽  
Christoph Boes ◽  
Edmund R.S. Kunji
Keyword(s):  
Membrane Protein ◽  
Protein Expression ◽  
Lactococcus Lactis ◽  
Membrane Protein Expression
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