The Application of Fluorescent Probes for the Analysis of Lipid Dynamics During Phagocytosis

2009 ◽  
pp. 121-134 ◽  
Author(s):  
Ronald S. Flannagan ◽  
Sergio Grinstein
Keyword(s):  
Fluorescent Probes ◽  
Lipid Dynamics

Confocal microscopy of the cytoskeleton in living plant cells following microinjection of fluorescent probes

1993 ◽  
Vol 51 ◽  
pp. 130-131
Author(s):  
Ann Cleary
Keyword(s):  
Cell Division ◽  
Fluorescent Probes ◽  
Actin Filaments ◽  
Intracellular Transport ◽  
Cell Structure ◽  
Plant Cells ◽  
Cell Plate ◽  
Cell Cortex ◽  
Living Plant ◽  
Rhodamine Phalloidin

Microinjection of fluorescent probes into living plant cells reveals new aspects of cell structure and function. Microtubules and actin filaments are dynamic components of the cytoskeleton and are involved in cell growth, division and intracellular transport. To date, cytoskeletal probes used in microinjection studies have included rhodamine-phalloidin for labelling actin filaments and fluorescently labelled animal tubulin for incorporation into microtubules. From a recent study of Tradescantia stamen hair cells it appears that actin may have a role in defining the plane of cell division. Unlike microtubules, actin is present in the cell cortex and delimits the division site throughout mitosis. Herein, I shall describe actin, its arrangement and putative role in cell plate placement, in another material, living cells of Tradescantia leaf epidermis.The epidermis is peeled from the abaxial surface of young leaves usually without disruption to cytoplasmic streaming or cell division. The peel is stuck to the base of a well slide using 0.1% polyethylenimine and bathed in a solution of 1% mannitol +/− 1 mM probenecid.

Live Confocal Analysis of Fertilization and Early Development

2001 ◽  
Vol 7 (S2) ◽  
pp. 1012-1013
Author(s):  
Uyen Tram ◽  
William Sullivan
Keyword(s):  
Drosophila Melanogaster ◽  
Embryonic Development ◽  
Real Time ◽  
Early Development ◽  
Fluorescent Probes ◽  
Primary Defect ◽  
Fluorescent Analysis ◽  
Dynamic Event ◽  
Enormous Amount ◽  
Fluorescent Studies

Embryonic development is a dynamic event and is best studied in live animals in real time. Much of our knowledge of the early events of embryogenesis, however, comes from immunofluourescent analysis of fixed embryos. While these studies provide an enormous amount of information about the organization of different structures during development, they can give only a static glimpse of a very dynamic event. More recently real-time fluorescent studies of living embryos have become much more routine and have given new insights to how different structures and organelles (chromosomes, centrosomes, cytoskeleton, etc.) are coordinately regulated. This is in large part due to the development of commercially available fluorescent probes, GFP technology, and newly developed sensitive fluorescent microscopes. For example, live confocal fluorescent analysis proved essential in determining the primary defect in mutations that disrupt early nuclear divisions in Drosophila melanogaster. For organisms in which GPF transgenics is not available, fluorescent probes that label DNA, microtubules, and actin are available for microinjection.

957: PSA & LIPID Dynamics in Hypogonadal Men Treated with Testosterone Replacement Therapy

2007 ◽  
Vol 177 (4S) ◽  
pp. 316-317
Author(s):  
Jay Simhan ◽  
R. Matthew Coward ◽  
Culley C. Carson
Keyword(s):  
Replacement Therapy ◽  
Testosterone Replacement ◽  
Testosterone Replacement Therapy ◽  
Lipid Dynamics

Lipid dynamics in the annexin V - membrane complex

1999 ◽  
Vol 96 (9/10) ◽  
pp. 1602-1607
Author(s):  
O. Saurel ◽  
P. Demange ◽  
A. Lopez ◽  
A. Milon
Keyword(s):  
Annexin V ◽  
Membrane Complex ◽  
Lipid Dynamics

Three-stage lipid dynamics during development of planktotrophic echinoderm larvae

2017 ◽  
Vol 583 ◽  
pp. 149-161 ◽  
Author(s):  
TAA Prowse ◽  
MA Sewell ◽  
M Byrne
Keyword(s):  
Lipid Dynamics

Seasonal lipid dynamics of the shrimps Crangon crangon and Pandalus montagui in the German Bight (North Sea)

2019 ◽  
Vol 625 ◽  
pp. 41-52 ◽  
Author(s):  
D Martínez-Alarcón ◽  
R Saborowski ◽  
E Melis ◽  
W Hagen
Keyword(s):  
North Sea ◽  
German Bight ◽  
Crangon Crangon ◽  
Lipid Dynamics

Characterization of the Interactions of Fluorescent Probes with Proteins: Coumarin 153 and 1,8-ANS in Complex with Holo- and Apomyoglobin

2006 ◽  
Vol 82 (6) ◽  
pp. 1586 ◽  
Author(s):  
Prasun Mukherjee ◽  
Mintu Halder ◽  
Mark S. Hargrove ◽  
Jacob W. Petrich
Keyword(s):  
Fluorescent Probes ◽  
Coumarin 153

1714-P: Regulation of Phosphorylation Status in Insulin Signaling via a4 Is Necessary for Adipocyte Maintenance and Lipid Dynamics

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 1714-P
Author(s):  
MASAJI SAKAGUCHI ◽  
SHOTA OKAGAWA ◽  
YUMA OKUBO ◽  
SAYAKA KITANO ◽  
MOTOYUKI IGATA ◽  
...  
Keyword(s):  
Insulin Signaling ◽  
Lipid Dynamics
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