Preparation of Lipid Membrane Surfaces for Molecular Interaction Studies by Surface Plasmon Resonance Biosensors

2010 ◽  
pp. 191-200 ◽  
Author(s):  
Mojca Podlesnik Beseničar ◽  
Gregor Anderluh
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Molecular Interaction ◽  
Lipid Membrane ◽  
Membrane Surfaces ◽  
Interaction Studies

scholarly journals Surface plasmon resonance biosensor for exosome detection based on reformative tyramine signal amplification activated by molecular aptamer beacon

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Wenqin Chen ◽  
Zhiyang Li ◽  
Wenqian Cheng ◽  
Tao Wu ◽  
Jia Li ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Signal Amplification ◽  
Lipid Membrane ◽  
Breast Cancer Patients ◽  
Her2 Positive ◽  
Chip Surface ◽  
Spr Biosensor ◽  
Aptamer Beacon

AbstractHuman epidermal growth factor receptor 2 (HER2)-positive exosomes play an extremely important role in the diagnosis and treatment options of breast cancers. Herein, based on the reformative tyramine signal amplification (TSA) enabled by molecular aptamer beacon (MAB) conversion, a label-free surface plasmon resonance (SPR) biosensor was proposed for highly sensitive and specific detection of HER2-positive exosomes. The exosomes were captured by the HER2 aptamer region of MAB immobilized on the chip surface, which enabled the exposure of the G-quadruplex DNA (G4 DNA) that could form peroxidase-like G4-hemin. In turn, the formed G4-hemin catalyzed the deposition of plentiful tyramine-coated gold nanoparticles (AuNPs-Ty) on the exosome membrane with the help of H2O2, generating a significantly enhanced SPR signal. In the reformative TSA system, the horseradish peroxidase (HRP) as a major component was replaced with nonenzymic G4-hemin, bypassing the defects of natural enzymes. Moreover, the dual-recognition of the surface proteins and lipid membrane of the desired exosomes endowed the sensing strategy with high specificity without the interruption of free proteins. As a result, this developed SPR biosensor exhibited a wide linear range from 1.0 × 104 to 1.0 × 107 particles/mL. Importantly, this strategy was able to accurately distinguish HER2-positive breast cancer patients from healthy individuals, exhibiting great potential clinical application. Graphical Abstract

scholarly journals Surface functionalization of SPR chip for specific molecular interaction analysis under flow condition

2017 ◽  
Vol 10 (02) ◽  
pp. 1650040 ◽  
Author(s):  
Tao Ma ◽  
Xiaoxia Chen ◽  
Qing Peng ◽  
Pengfei Zhang ◽  
Yonghong He
Keyword(s):  
Aqueous Solution ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Surface Functionalization ◽  
Plasmon Resonance ◽  
Molecular Interaction ◽  
Flow Condition ◽  
Interaction Analysis ◽  
Low Cost ◽  
Protein A

Surface functionalization of sensor chip for probe immobilization is crucial for the biosensing applications of surface plasmon resonance (SPR) sensors. In this paper, we report a method circulating the dopamine aqueous solution to coat polydopamine film on sensing surface for surface functionalization of SPR chip. The polydopamine film with available thickness can be easily prepared by controlling the circulation time and the biorecognition elements can be immobilized on the polydopamine film for specific molecular interaction analysis. These operations are all performed under flow condition in the fluidic system, and have the advantages of easy implementation, less time consuming, and low cost, because the reagents and devices used in the operations are routinely applied in most laboratories. In this study, the specific absorption between the protein A probe immobilized on the sensing surface and human immunoglobulin G in the buffer is monitored based on this surface functionalization strategy to demonstrated its feasibility for SPR biosensing applications.

Surface Plasmon Resonance Spectroscopic Study on Pore-Forming Behavior of Streptolysin O on Supported Phospholipid Bilayers

2003 ◽  
Vol 774 ◽  
Author(s):  
Thomas Wilkop ◽  
Danke Xu ◽  
Quan Cheng
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Lipid Membrane ◽  
Bilayer Membrane ◽  
Membrane Properties ◽  
Toxin Binding ◽  
Streptolysin O ◽  
Spacer Layer ◽  
Biomimetic Membrane

AbstractA novel supported bilayer membrane was formed on top of a hydrophobic spacer layer of hexyl thioctate (HT) and its application in the detection of pore forming toxins was demonstrated. The formation kinetics, effect of different flow rates on the fusion process of vesicles and perforation by incubation with streptolysin O (SLO) and pristine membranes were investigated. Individual layers of the multilayer structure were successfully characterized with surface plasmon resonance (SPR), and the resulting spectra were analyzed in order to determine the layer thickness and other membrane properties. The interaction of SLO with the pristine biomimetic membrane resulted in a pronounced change in the reflectivity spectrum. Elements of these spectral changes were analyzed to explain the process of toxin binding and pore formation taking place on the lipid membrane.

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