Application of Full-Length cDNA Resources to Gain-of-Function Technology for Characterization of Plant Gene Function

2011 ◽  
pp. 183-197 ◽  
Author(s):  
Youichi Kondou ◽  
Mieko Higuchi ◽  
Takanari Ichikawa ◽  
Minami Matsui
Keyword(s):  
Gene Function ◽  
Full Length ◽  
Gain Of Function ◽  
Full Length Cdna ◽  
Plant Gene

Cloning and characterization of full-length cDNA clones encoding chalcone synthase from the orchid Bromheadia finlaysoniana

1998 ◽  
Vol 36 (9) ◽  
pp. 647-656 ◽  
Author(s):  
Chye-Fong Liew ◽  
Chong-Jin Goh ◽  
Chiang-Shiong Loh ◽  
Saw-Hoon Lim
Keyword(s):  
Chalcone Synthase ◽  
Full Length ◽  
Cdna Clones ◽  
Full Length Cdna

scholarly journals Characterization of a pathogenic full-length cDNA clone of a virulent porcine epidemic diarrhea virus strain AH2012/12 in China

Virology ◽  
2017 ◽  
Vol 500 ◽  
pp. 50-61 ◽  
Author(s):  
Baochao Fan ◽  
Zhengyu Yu ◽  
Fengjiao Pang ◽  
Xiangwei Xu ◽  
Baimeng Zhang ◽  
...  
Keyword(s):  
Virus Strain ◽  
Cdna Clone ◽  
Porcine Epidemic Diarrhea Virus ◽  
Full Length ◽  
Diarrhea Virus ◽  
Full Length Cdna ◽  
Porcine Epidemic Diarrhea

Characterization of 2159 Unmapped Full-length cDNA Sequences of Oryza sativa L. ssp. japonica ‘Nipponbare’

2009 ◽  
Vol 28 (2) ◽  
pp. 357-362
Author(s):  
Hiroshi Mizuno ◽  
Tsuyoshi Tanaka ◽  
Hiroaki Sakai ◽  
Hiroyuki Kawahigashi ◽  
Takeshi Itoh ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Full Length ◽  
Full Length Cdna ◽  
Cdna Sequences

scholarly journals Rat P45017αfrom Testis: Characterization of a Full-Length cDNA Encoding a Unique Steroid Hydroxylase Capable of Catalyzing Both Δ4- and Δ5-Steroid-17,20-Lyase Reactions

1989 ◽  
Vol 3 (6) ◽  
pp. 968-975 ◽  
Author(s):  
H. Richard Fevold ◽  
Matthew C. Lorence ◽  
John L. McCarthy ◽  
John M. Trant ◽  
Masaaki Kagimoto ◽  
...  
Keyword(s):  
Full Length ◽  
Full Length Cdna ◽  
Steroid Hydroxylase

scholarly journals RiceFOX: A Database of Arabidopsis Mutant Lines Overexpressing Rice Full-Length cDNA that Contains a Wide Range of Trait Information to Facilitate Analysis of Gene Function

2010 ◽  
Vol 52 (2) ◽  
pp. 265-273 ◽  
Author(s):  
Tetsuya Sakurai ◽  
Youichi Kondou ◽  
Kenji Akiyama ◽  
Atsushi Kurotani ◽  
Mieko Higuchi ◽  
...  
Keyword(s):  
Gene Function ◽  
Full Length ◽  
Full Length Cdna ◽  
Wide Range ◽  
Arabidopsis Mutant ◽  
Mutant Lines

CHARACTERIZATION OF THE SPLICE SITES IN GT–AG AND GC–AG INTRONS IN HIGHER EUKARYOTES USING FULL-LENGTH cDNAs

2004 ◽  
Vol 02 (02) ◽  
pp. 309-331 ◽  
Author(s):  
SUMIE KITAMURA–ABE ◽  
HITOMI ITOH ◽  
TAKANORI WASHIO ◽  
AKIHIRO TSUTSUMI ◽  
MASARU TOMITA
Keyword(s):  
Alternative Splice ◽  
Splice Site ◽  
Consensus Sequence ◽  
Full Length ◽  
Splice Sites ◽  
Consensus Sequences ◽  
Full Length Cdna ◽  
Strong Consensus ◽  
Higher Eukaryotes

For the purpose of analyzing the relation between the splice sites and the order of introns, we conducted the following analysis for the GT–AG and GC–AG splice site groups. First, the pre-mRNAs of H. sapiens, M. musculus, D. melanogaster, A. thaliana and O. sativa were sampled by mapping the full-length cDNA to the genomes. Next, the consensus sequences at different regions of pre-mRNAs were analyzed in the five species. We also investigated the mononucleotide and dinucleotide frequencies in the extensive regions around the 5' splice sites (5'ss) and 3' splice sites (3'ss). As a result, differential frequencies of nucleotides at the first 5'ss in both the GT–AG and GC–AG splice site groups were observed in A. thaliana and O. sativa pre-mRNAs. The trend, which indicates that GC 5'ss possess strong consensus sequences, was observed not only in mammalian pre-mRNAs but also in the pre-mRNAs of D. melanogaster, A. thaliana and O. sativa. Furthermore, we examined the consensus sequences of the constitutive and alternative splice sites. It was suggested that in the case of the alternative GC–AG introns, the tendency to have a weak consensus sequence at 5'ss is different between H. sapiens and M. musculus pre-mRNAs.

CHARACTERIZATION OF A FULL-LENGTH cDNA FOR RABBIT FACTOR X

1997 ◽  
Vol 85 (6) ◽  
pp. 503-514 ◽  
Author(s):  
Usha R Pendurthi ◽  
Kimberly D Anderson ◽  
Harold L James
Keyword(s):  
Full Length ◽  
Factor X ◽  
Full Length Cdna
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