Detection of Neisseria meningitidis in Cerebrospinal Fluid Using a Multiplex PCR and the Luminex Detection Technology

2011 ◽  
pp. 37-53 ◽  
Author(s):  
Jens Kjølseth Møller
Keyword(s):  
Cerebrospinal Fluid ◽  
Neisseria Meningitidis ◽  
Multiplex Pcr ◽  
Detection Technology

scholarly journals Use of a New Single Multiplex PCR-Based Assay for Direct Simultaneous Characterization of Six Neisseria meningitidis Serogroups

2009 ◽  
Vol 47 (8) ◽  
pp. 2662-2666 ◽  
Author(s):  
C. Fraisier ◽  
R. Stor ◽  
B. Tenebray ◽  
Y. Sanson ◽  
P. Nicolas
Keyword(s):  
Neisseria Meningitidis ◽  
Multiplex Pcr

scholarly journals Study of 138 Neisseria meningitidis strains isolated from blood or cerebrospinal fluid in Lombardy between 2007 and 2010

2012 ◽  
Vol 27 (2) ◽  
Author(s):  
Laura Daprai ◽  
Maria Laura Garlaschi ◽  
Giuseppina Scelza ◽  
Damiano Picicco ◽  
Maria Cristina Garlaschi ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Neisseria Meningitidis

Interactions between Neisseria meningitidis and human cells that promote colonisation and disease

2004 ◽  
Vol 6 (14) ◽  
pp. 1-14 ◽  
Author(s):  
Anne Corbett ◽  
Rachel Exley ◽  
Sandrine Bourdoulous ◽  
Christoph M. Tang
Keyword(s):  
Cerebrospinal Fluid ◽  
Bacterial Meningitis ◽  
Neisseria Meningitidis ◽  
Human Cell ◽  
Molecular Basis ◽  
Cell Types ◽  
Cellular Level ◽  
Human Cells ◽  
Healthy Individuals ◽  
Host Pathogen

Neisseria meningitidis is the leading cause of bacterial meningitis, a potentially fatal condition that particularly affects children. Multiple steps are involved during the pathogenesis of infection, including the colonisation of healthy individuals and invasion of the bacterium into the cerebrospinal fluid. The bacterium is capable of adhering to, and entering into, a range of human cell types, which facilitates its ability to cause disease. This article summarises the molecular basis of host–pathogen interactions at the cellular level during meningococcal carriage and disease.

Susceptibilities of invasive Neisseria meningitidis strains to agents used for prophylaxis and to penicillin G

2021 ◽  
pp. e20200034
Author(s):  
Carrie Phillips ◽  
David JM Haldane
Keyword(s):  
Cerebrospinal Fluid ◽  
Nova Scotia ◽  
Neisseria Meningitidis ◽  
Antimicrobial Susceptibility ◽  
Penicillin G ◽  
Minimal Inhibitory Concentrations ◽  
Sheep Blood ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

Antimicrobial susceptibility of 50 Neisseria meningitidis strains detected in Nova Scotia between 2004 and 2018 was determined. The isolates were cultured from sites that might prompt chemoprophylaxis (27 blood, 18 cerebrospinal fluid [CSF], 3 CSF–blood, and 2 conjunctiva). Minimal inhibitory concentrations (MICs) were determined to azithromycin, ciprofloxacin, minocycline, rifampin, trimethoprim–sulfamethoxazole, and penicillin G, using a diffusion gradient strip on Mueller–Hinton agar with 5% sheep blood in 5% CO2 for 20–24 hours. All isolates remained susceptible to azithromycin, ciprofloxacin, minocycline, and rifampin, but there was 26% resistance to trimethoprim–sulfamethoxazole. There was a rise in penicillin MIC of the isolates over the study period.

Utility of FilmArrayⓇ ME panel for prompt Neisseria meningitidis detection in non-cerebrospinal fluid samples– A case report

2020 ◽  
Vol 80 (1) ◽  
pp. 121-142
Author(s):  
Paloma García-Clemente ◽  
Juan José Menéndez-Suso ◽  
Iker Falces-Romero ◽  
Luis Escosa-García ◽  
Cristina Schüffelmann ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Case Report ◽  
Neisseria Meningitidis

scholarly journals Bacterial antigen detection in cerebrospinal fluid by the latex agglutination test

1995 ◽  
Vol 37 (3) ◽  
pp. 257-260 ◽  
Author(s):  
Ilka Maria Landgraf ◽  
Maria das Graças Adelino Alkmin ◽  
Maria de Fátima Paiva Vieira
Keyword(s):  
Cerebrospinal Fluid ◽  
Neisseria Meningitidis ◽  
Clinical Evidence ◽  
Latex Agglutination ◽  
Haemophilus Influenzae Type ◽  
Bacterial Antigen ◽  
Haemophilus Influenzae Type B ◽  
Type B ◽  
Gram Stain ◽  
Comparison Of The Results

Eighty purulent cerebrospinal fluid (CSF) samples from patients with clinical evidence of meningitis were studied using the Directigen latex agglutination (LA) kit to determine the presence of bacterial antigen in CSF. The results showed a better diagnostic performance of the LA test than bacterioscopy by Gram stain, culture and counterimmunoelectrophoresis (CIE), as far as Neisseria meningitidis groups B and C, and Haemophilus influenzae type b are concerned, and a better performance than bacterioscopy and culture considering Streptococcus pneumoniae. Comparison of the results with those of culture showed that the LA test had the highest sensitivity for the Neisseria meningitidis group C. Comparing the results with those of CIE, the highest levels of sensitivity were detected for N. meningitidis groups B and C. Regarding specificity, fair values were obtained for all organisms tested. The degree of K agreement when the LA test was compared with CIE exhibited better K indices of agreement for N. meningitidis groups B and C.

Pili and Outer Membrane Appendages on Neisseria meningitidis in the Cerebrospinal Fluid of an Infant

1982 ◽  
Vol 146 (4) ◽  
pp. 568-568 ◽  
Author(s):  
D. S. Stephens ◽  
K. M. Edwards ◽  
F. Morris ◽  
Z. A. McGee
Keyword(s):  
Cerebrospinal Fluid ◽  
Neisseria Meningitidis ◽  
Outer Membrane
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