High-Throughput Yeast Two-Hybrid Screening

2011 ◽  
pp. 39-61 ◽  
Author(s):  
George G. Roberts ◽  
Jodi R. Parrish ◽  
Bernardo A. Mangiola ◽  
Russell L. Finley
Keyword(s):  
High Throughput ◽  
Yeast Two Hybrid ◽  
Two Hybrid ◽  
Hybrid Screening

Abstract 3033: Identification of the MUC16/CA125 interaction network: Results of a high-throughput yeast two-hybrid screening

2012 ◽  
Author(s):  
Stephan Polterauer ◽  
Dharmarao Thapi ◽  
Nikolaus Schultz ◽  
Ouathek Ouerfelli ◽  
Nancy Chen ◽  
...  
Keyword(s):  
High Throughput ◽  
Interaction Network ◽  
Yeast Two Hybrid ◽  
Two Hybrid ◽  
Hybrid Screening

Interaction network of antimicrobial peptides of Arabidopsis thaliana, based on high-throughput yeast two-hybrid screening

2012 ◽  
Vol 58 ◽  
pp. 245-252 ◽  
Author(s):  
Coralie Damon ◽  
Julia Dmitrieva ◽  
Yordan Muhovski ◽  
Frédéric Francis ◽  
Laurence Lins ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Antimicrobial Peptides ◽  
High Throughput ◽  
Interaction Network ◽  
Yeast Two Hybrid ◽  
Two Hybrid ◽  
Hybrid Screening

Development of a high-throughput yeast two-hybrid screening system to study protein-protein interactions in plants

2002 ◽  
Vol 267 (2) ◽  
pp. 142-153 ◽  
Author(s):  
Y. Fang ◽  
D. Macool ◽  
Z. Xue ◽  
E. Heppard ◽  
C. Hainey ◽  
...  
Keyword(s):  
High Throughput ◽  
Protein Interactions ◽  
Screening System ◽  
Protein Protein Interactions ◽  
Yeast Two Hybrid ◽  
Two Hybrid ◽  
Hybrid Screening

A surface display yeast two-hybrid screening system for high-throughput protein interactome mapping

2009 ◽  
Vol 390 (1) ◽  
pp. 29-37 ◽  
Author(s):  
Jun Chen ◽  
Jianhong Zhou ◽  
Claire K. Sanders ◽  
John P. Nolan ◽  
Hong Cai
Keyword(s):  
High Throughput ◽  
Surface Display ◽  
Screening System ◽  
Yeast Two Hybrid ◽  
Protein Interactome ◽  
Interactome Mapping ◽  
Two Hybrid ◽  
Hybrid Screening

scholarly journals Two LIM Domain Proteins and UNC-96 Link UNC-97/PINCH to Myosin Thick Filaments in Caenorhabditis elegans Muscle

2007 ◽  
Vol 18 (11) ◽  
pp. 4317-4326 ◽  
Author(s):  
Hiroshi Qadota ◽  
Kristina B. Mercer ◽  
Rachel K. Miller ◽  
Kozo Kaibuchi ◽  
Guy M. Benian
Keyword(s):  
Caenorhabditis Elegans ◽  
Binding Assays ◽  
Lim Domain ◽  
Yeast Two Hybrid ◽  
Lim Domains ◽  
Thick Filaments ◽  
Vitro Binding ◽  
Two Hybrid ◽  
Hybrid Screening

By yeast two-hybrid screening, we found three novel interactors (UNC-95, LIM-8, and LIM-9) for UNC-97/PINCH in Caenorhabditis elegans. All three proteins contain LIM domains that are required for binding. Among the three interactors, LIM-8 and LIM-9 also bind to UNC-96, a component of sarcomeric M-lines. UNC-96 and LIM-8 also bind to the C-terminal portion of a myosin heavy chain (MHC), MHC A, which resides in the middle of thick filaments in the proximity of M-lines. All interactions identified by yeast two-hybrid assays were confirmed by in vitro binding assays using purified proteins. All three novel UNC-97 interactors are expressed in body wall muscle and by antibodies localize to M-lines. Either a decreased or an increased dosage of UNC-96 results in disorganization of thick filaments. Our previous studies showed that UNC-98, a C2H2 Zn finger protein, acts as a linkage between UNC-97, an integrin-associated protein, and MHC A in myosin thick filaments. In this study, we demonstrate another mechanism by which this linkage occurs: from UNC-97 through LIM-8 or LIM-9/UNC-96 to myosin.

scholarly journals Construction of gateway-compatible yeast two-hybrid vectors for high throughput analysis of protein interaction

2010 ◽  
Vol 9 (9) ◽  
pp. 1392-1396
Author(s):  
Zhu Tingheng ◽  
Wang Weixia ◽  
Wong Hann lin ◽  
Yang Xiao ◽  
Wang Kun ◽  
...  
Keyword(s):  
High Throughput ◽  
Protein Interaction ◽  
Yeast Two Hybrid ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Hybrid Vectors ◽  
Two Hybrid
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