Perfusion Culture of Mammalian Cells in a Microfluidic Channel with a Built-In Pillar Array

2012 ◽  
pp. 83-94
Author(s):  
Chi Zhang
Keyword(s):  
Mammalian Cells ◽  
Microfluidic Channel ◽  
Perfusion Culture ◽  
Pillar Array

Error propagation from prime variables into specific rates and metabolic fluxes for mammalian cells in perfusion culture

2009 ◽  
Vol 25 (4) ◽  
pp. 986-998 ◽  
Author(s):  
Chetan T. Goudar ◽  
Richard Biener ◽  
Konstantin B. Konstantinov ◽  
James M. Piret
Keyword(s):  
Error Propagation ◽  
Mammalian Cells ◽  
Perfusion Culture ◽  
Metabolic Fluxes

Perfusion culture apparatus for suspended mammalian cells

1993 ◽  
Vol 13 (3) ◽  
pp. 149-159 ◽  
Author(s):  
Michiyuki Tokashiki ◽  
Hiroyuki Takamatsu
Keyword(s):  
Mammalian Cells ◽  
Perfusion Culture

Solution to the high Dissolved CO2 Problem in High-Density Perfusion Culture of Mammalian Cells

2001 ◽  
pp. 399-402 ◽  
Author(s):  
R. Matanguihan ◽  
E. Sajan ◽  
M. Zachariou ◽  
C. Olson ◽  
Jim Michaels ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Perfusion Culture ◽  
High Density

Large-scale perfusion culture process for suspended mammalian cells that uses a centrifuge with multiple settling zones

1996 ◽  
Vol 45 (4) ◽  
pp. 454-457
Author(s):  
H. Takamatsu ◽  
K. Hamamoto ◽  
K. Ishimaru ◽  
S. Yokoyama ◽  
M. Tokashiki
Keyword(s):  
Mammalian Cells ◽  
Large Scale ◽  
Perfusion Culture ◽  
Culture Process

Potential of cell retention techniques for large-scale high-density perfusion culture of suspended mammalian cells

2003 ◽  
Vol 82 (7) ◽  
pp. 751-765 ◽  
Author(s):  
D. Voisard ◽  
F. Meuwly ◽  
P.-A. Ruffieux ◽  
G. Baer ◽  
A. Kadouri
Keyword(s):  
Mammalian Cells ◽  
Large Scale ◽  
Perfusion Culture ◽  
High Density ◽  
Cell Retention

A practical guide to microfluidic perfusion culture of adherent mammalian cells

Lab on a Chip ◽  
2007 ◽  
Vol 7 (6) ◽  
pp. 681 ◽  
Author(s):  
Lily Kim ◽  
Yi-Chin Toh ◽  
Joel Voldman ◽  
Hanry Yu
Keyword(s):  
Mammalian Cells ◽  
Perfusion Culture ◽  
Practical Guide ◽  
Microfluidic Perfusion ◽  
Adherent Mammalian Cells

Silk screen based dual spin-filter module for perfusion culture of adherent and non-adherent mammalian cells

2014 ◽  
Vol 36 (8) ◽  
pp. 1623-1630 ◽  
Author(s):  
Shweta Kamthan ◽  
James Gomes ◽  
Pradip K. Roychoudhury
Keyword(s):  
Mammalian Cells ◽  
Perfusion Culture ◽  
Spin Filter ◽  
Silk Screen ◽  
Adherent Mammalian Cells

scholarly journals Cell Stress Reduction by a Novel Perfusion-Culture System Using Commercial Culture Dish

2019 ◽  
Vol 10 (1) ◽  
pp. 95
Author(s):  
Reiko Yasuda ◽  
Shungo Adachi ◽  
Atsuhito Okonogi ◽  
Yohei Anzai ◽  
Tadataka Kamiyama ◽  
...  
Keyword(s):  
Culture System ◽  
Culture Media ◽  
Microfluidic Channel ◽  
Perfusion Culture ◽  
Uniform Flow ◽  
Complete Medium ◽  
Commercial Culture ◽  
Culture Dish ◽  
Waste Products ◽  
Medium Change

Two typical medium replacement methods are employed to culture cells, that is, traditional manual medium change, wherein complete medium changes are performed at short-term intervals; and perfusion, wherein continuous culture media delivery and discharge are performed. The former is associated with the issue of readily accumulation of metabolic waste products, which is resolved by the latter. However, the latter requires a specific chamber for cell culturing such as a microfluidic channel for flow stability. Generally, bio-medical researchers require a culturing process that utilizes commercial culture dishes to test their conventional manipulation know-how, experience, and protocols. Thus, we constructed a perfusion-culture system using commercial 35 mm culture dishes. To use commercial culture dishes, it is necessary to maintain the culturing conditions including a constant volume and uniform flow rate, and we invented a novel adapter, culture dish adapter (CD-Adapter), attached to a commercial culture dish. The CD-Adapter is fixed with a holding jig to keep the medium volume constant, so it can maintain a uniform flow in the culture dish. Additionally, we demonstrate the applicability of the system by applying it to a perfusion culture of 293T cells, revealing a 50% reduction in DDIT3, a cellular stress marker, compared with that in conventional manual medium change.

Sign in / Sign up

Export Citation Format

Share Document