Sperm DNA Damage Measured by Comet Assay

2012 ◽  
pp. 137-146 ◽  
Author(s):  
Luke Simon ◽  
Douglas T. Carrell
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Sperm Dna Damage ◽  
Sperm Dna

Sperm DNA damage output parameters measured by the alkaline Comet assay and their importance

Andrologia ◽  
2016 ◽  
Vol 49 (2) ◽  
pp. e12608 ◽  
Author(s):  
L. Simon ◽  
K. I. Aston ◽  
B. R. Emery ◽  
J. Hotaling ◽  
D. T. Carrell
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Alkaline Comet Assay ◽  
Sperm Dna Damage ◽  
Sperm Dna

scholarly journals Interpreting sperm DNA damage in a diverse range of mammalian sperm by means of the two-tailed comet assay

2014 ◽  
Vol 5 ◽  
Author(s):  
Elva I. Cortés-Gutiérrez ◽  
Carmen López-Fernández ◽  
José Luis Fernández ◽  
Martha I. Dávila-Rodríguez ◽  
Stephen D. Johnston ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Diverse Range ◽  
Sperm Dna Damage ◽  
Mammalian Sperm ◽  
Sperm Dna

scholarly journals Rapid rates of sperm DNA damage after activation in tench (Tinca tinca: Teleostei, Cyprinidae) measured using a sperm chromatin dispersion test

Reproduction ◽  
2009 ◽  
Vol 138 (2) ◽  
pp. 257-266 ◽  
Author(s):  
Carmen López-Fernández ◽  
Matthew J G Gage ◽  
Francisca Arroyo ◽  
Altea Gosálbez ◽  
Ana M Larrán ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Dna Fragmentation ◽  
Living Organism ◽  
Sperm Chromatin ◽  
Tinca Tinca ◽  
External Fertilization ◽  
Sperm Dna Damage ◽  
Sperm Dna ◽  
Dispersion Test

Spermatozoal haplotypic DNA is prone to damage, leading to male fertility problems. So far, the assessment of sperm DNA breakage has been challenging because protamines render the nuclear chromatin highly compacted. Here, we report the application of a new test to quantify DNA fragmentation in spermatozoa of an externally fertilizing teleost fish. The sperm chromatin dispersion (SCD) test uses a species-specific lysing solution to generate controlled protein depletion that, followed by DNA-specific fluorescent labelling, allows an easy morphological discrimination between nuclei affected by DNA damage. Using tench (Tinca tinca) as our model, we first trialled the test against established, but more technically demanding, assays employing in situ nick translation (ISNT) and the comet assay. The SCD test showed high concordance with ISNT, comet assay measures and a chromatin-swelling test, confirming the application of this straightforward SCD technique to various aspects of reproductive biology. Second, we examined between-male variation in DNA damage, and measured changes through time following spermatozoal activation. Between-male variation in the basal levels of average DNA damage ranged from 0 to 20% of sperm showing damage, and all showed increases in DNA fragmentation through time (0–60 min). The rates of DNA damage increase are the fastest so far recorded in sperm for a living organism, and may relate to the external fertilization mode. Our findings have relevance for broodstock selection and optimizing IVF protocols routinely used in modern aquaculture.

scholarly journals Alkaline and neutral Comet assay profiles of sperm DNA damage in clinical groups

2012 ◽  
Vol 27 (3) ◽  
pp. 652-658 ◽  
Author(s):  
J. Ribas-Maynou ◽  
A. Garcia-Peiro ◽  
C. Abad ◽  
M. J. Amengual ◽  
J. Navarro ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Sperm Dna Damage ◽  
Neutral Comet Assay ◽  
Sperm Dna

scholarly journals Human sperm sex chromosome disomy and sperm DNA damage assessed by the neutral comet assay

2014 ◽  
Vol 29 (10) ◽  
pp. 2148-2155 ◽  
Author(s):  
M. E. McAuliffe ◽  
P. L. Williams ◽  
S. A. Korrick ◽  
R. Dadd ◽  
F. Marchetti ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Sex Chromosome ◽  
Human Sperm ◽  
Sperm Dna Damage ◽  
Neutral Comet Assay ◽  
Sperm Dna
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