Detection of In Vivo Mutation in the Hprt and Pig-a Genes of Rat Lymphocytes

2013 ◽  
pp. 79-95 ◽  
Author(s):  
Vasily N. Dobrovolsky ◽  
Joseph G. Shaddock ◽  
Roberta A. Mittelstaedt ◽  
Daishiro Miura ◽  
Robert H. Heflich
Keyword(s):  
Rat Lymphocytes

O6-methylguanine-DNA adducts in rat lymphocytes after in vivo exposure to N-nitrosodimethylamine (NDMA)

1994 ◽  
Vol 16 (7) ◽  
pp. 583-591 ◽  
Author(s):  
S. Fadlallah ◽  
M. Lachapelle ◽  
K. Krzystyniak ◽  
S. Cooper ◽  
F. Denizeau ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Rat Lymphocytes

Detection of In Vivo Mutation in the Hprt and Pig-a Genes of Rat Lymphocytes

2019 ◽  
pp. 59-75
Author(s):  
Vasily N. Dobrovolsky ◽  
Joseph G. Shaddock ◽  
Roberta A. Mittelstaedt ◽  
Daishiro Miura ◽  
Robert H. Heflich
Keyword(s):  
Rat Lymphocytes

scholarly journals Proliferation in vivo of T lymphocytes reactive to histocompatibility alloantigens: a correction.

1975 ◽  
Vol 142 (1) ◽  
pp. 230-235 ◽  
Author(s):  
P C Nowell ◽  
J B Finan ◽  
D B Wilson
Keyword(s):  
Life History ◽  
Peripheral Blood ◽  
Tritiated Thymidine ◽  
Age Distribution ◽  
Blood Cultures ◽  
Lymphocyte Population ◽  
Rat Lymphocytes ◽  
History Of ◽  
Distribution In Blood

Rat lymphocytes in mixed cultures can reutilize tritiated thymidine from labeled granulocytes. Shortly after thymidine injections in vivo, major effects on the frequency of labeled lymphocyte mitoses in peripheral blood cultures are introduced by 10-20% polymorph contamination, even though transfer of label via supernates is not demonstrable. Cold thymidine in the cultures prevents reutilization, and has permitted reevaluation of several previous conclusions concerning the life history of lymphocytes reactive to major histocompatibility alloantigens (HARC). Rather than being predominantly recently divided cells, HARC do not appear to have an age distribution, in blood or lymph, significantly different from the general recirculating lymphocyte population. However, the ability of immunization across strong allogeneic differences to increase markedly the proportion of young HARC among the specifically responsive population has been confirmed.

Further characterization of the antigen defined by the monoclonal antibody M27

1989 ◽  
Vol 94 (4) ◽  
pp. 725-731
Author(s):  
M.E. Bramwell ◽  
S.M. Humm
Keyword(s):  
Monoclonal Antibody ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Reducing Conditions ◽  
Rat Lymphocytes ◽  
Foetal Liver ◽  
Cultivation In Vitro

Using immunoblotting techniques, the antigen that binds the monoclonal antibody M27 has been clearly defined in terms of apparent molecular mass and distribution. In reducing conditions it has an apparent mass of 178K (K = 10(3) Mr) and is present in the cytoplasm and membranes of all mammalian tissue culture cells so far examined. It is absent from lines derived from avian, piscine and amphibian sources. It is also absent from foetal liver of both rat and mouse, but subsequently appears after cultivation in vitro. Similarly, it can be detected on rat lymphocytes only after mitogenic stimulation. However, it is found on both hepatoma and lymphoma cells in vitro, and on in vivo tumours from murine sources. It thus appears to be associated with cell proliferation.

In vitro and in vivo uptake of nickel sulfides by rat lymphocytes

1991 ◽  
Vol 65 (4) ◽  
pp. 324-329 ◽  
Author(s):  
Hartmut F. Hildebrand ◽  
Anne-Marie Decaestecker ◽  
Fatima-Zohra Arrouijal ◽  
Robert Martinez
Keyword(s):  
Rat Lymphocytes ◽  
In Vivo Uptake

Analysis of cytogenetic damage in rat lymphocytes following in vivo exposure to nitrobenzene

1983 ◽  
Vol 18 (3) ◽  
pp. 219-226 ◽  
Author(s):  
A.D. Kligerman ◽  
G.L. Erexson ◽  
J.L. Wilmer ◽  
M.C. Phelps
Keyword(s):  
Cytogenetic Damage ◽  
Rat Lymphocytes
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