Detection of In Vivo Mutation in the Hprt and Pig-a Genes of Rat Lymphocytes

2019 ◽  
pp. 59-75
Author(s):  
Vasily N. Dobrovolsky ◽  
Joseph G. Shaddock ◽  
Roberta A. Mittelstaedt ◽  
Daishiro Miura ◽  
Robert H. Heflich
Keyword(s):  
Rat Lymphocytes

O6-methylguanine-DNA adducts in rat lymphocytes after in vivo exposure to N-nitrosodimethylamine (NDMA)

1994 ◽  
Vol 16 (7) ◽  
pp. 583-591 ◽  
Author(s):  
S. Fadlallah ◽  
M. Lachapelle ◽  
K. Krzystyniak ◽  
S. Cooper ◽  
F. Denizeau ◽  
...  
Keyword(s):  
Dna Adducts ◽  
Rat Lymphocytes

scholarly journals Proliferation in vivo of T lymphocytes reactive to histocompatibility alloantigens: a correction.

1975 ◽  
Vol 142 (1) ◽  
pp. 230-235 ◽  
Author(s):  
P C Nowell ◽  
J B Finan ◽  
D B Wilson
Keyword(s):  
Life History ◽  
Peripheral Blood ◽  
Tritiated Thymidine ◽  
Age Distribution ◽  
Blood Cultures ◽  
Lymphocyte Population ◽  
Rat Lymphocytes ◽  
History Of ◽  
Distribution In Blood

Rat lymphocytes in mixed cultures can reutilize tritiated thymidine from labeled granulocytes. Shortly after thymidine injections in vivo, major effects on the frequency of labeled lymphocyte mitoses in peripheral blood cultures are introduced by 10-20% polymorph contamination, even though transfer of label via supernates is not demonstrable. Cold thymidine in the cultures prevents reutilization, and has permitted reevaluation of several previous conclusions concerning the life history of lymphocytes reactive to major histocompatibility alloantigens (HARC). Rather than being predominantly recently divided cells, HARC do not appear to have an age distribution, in blood or lymph, significantly different from the general recirculating lymphocyte population. However, the ability of immunization across strong allogeneic differences to increase markedly the proportion of young HARC among the specifically responsive population has been confirmed.

Captopril (SQ 14,225): In vitro and in vivo influence on the proliferative response of rat lymphocytes

1982 ◽  
Vol 38 (3) ◽  
pp. 399-401 ◽  
Author(s):  
Lise Binderup ◽  
E. Bramm ◽  
E. Arrigoni-Martelli
Keyword(s):  
Proliferative Response ◽  
Rat Lymphocytes

Further characterization of the antigen defined by the monoclonal antibody M27

1989 ◽  
Vol 94 (4) ◽  
pp. 725-731
Author(s):  
M.E. Bramwell ◽  
S.M. Humm
Keyword(s):  
Monoclonal Antibody ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Reducing Conditions ◽  
Rat Lymphocytes ◽  
Foetal Liver ◽  
Cultivation In Vitro

Using immunoblotting techniques, the antigen that binds the monoclonal antibody M27 has been clearly defined in terms of apparent molecular mass and distribution. In reducing conditions it has an apparent mass of 178K (K = 10(3) Mr) and is present in the cytoplasm and membranes of all mammalian tissue culture cells so far examined. It is absent from lines derived from avian, piscine and amphibian sources. It is also absent from foetal liver of both rat and mouse, but subsequently appears after cultivation in vitro. Similarly, it can be detected on rat lymphocytes only after mitogenic stimulation. However, it is found on both hepatoma and lymphoma cells in vitro, and on in vivo tumours from murine sources. It thus appears to be associated with cell proliferation.

In vitro and in vivo uptake of nickel sulfides by rat lymphocytes

1991 ◽  
Vol 65 (4) ◽  
pp. 324-329 ◽  
Author(s):  
Hartmut F. Hildebrand ◽  
Anne-Marie Decaestecker ◽  
Fatima-Zohra Arrouijal ◽  
Robert Martinez
Keyword(s):  
Rat Lymphocytes ◽  
In Vivo Uptake

In vivo imaging of rat lymphocytes with an indium 111-labelled anti-T cell monoclonal antibody: a comparison with indium 111-labelled lymphocytes

1990 ◽  
Vol 16 (2) ◽  
pp. 69-76 ◽  
Author(s):  
Issa Loutfi ◽  
Patricia M. Chisholm ◽  
Debbie Bevan ◽  
John P. Lavender
Keyword(s):  
Monoclonal Antibody ◽  
T Cell ◽  
In Vivo Imaging ◽  
Rat Lymphocytes ◽  
Indium 111

Analysis of cytogenetic damage in rat lymphocytes following in vivo exposure to nitrobenzene

1983 ◽  
Vol 18 (3) ◽  
pp. 219-226 ◽  
Author(s):  
A.D. Kligerman ◽  
G.L. Erexson ◽  
J.L. Wilmer ◽  
M.C. Phelps
Keyword(s):  
Cytogenetic Damage ◽  
Rat Lymphocytes

scholarly journals Protein Synthesis in Rat Lymphocytes: Radioautographic Studies of Availability and Utilization of Labeled Amino Acids in Vivo

Blood ◽  
1964 ◽  
Vol 23 (4) ◽  
pp. 502-516 ◽  
Author(s):  
WILLIAM O. RIEKE ◽  
M. ROY SCHWARZ
Keyword(s):  
Body Weight ◽  
Protein Synthesis ◽  
Lymph Node ◽  
Thoracic Duct ◽  
Thoracic Duct Lymph ◽  
Short Intervals ◽  
Daughter Cells ◽  
Rat Lymphocytes ◽  
Duct Lymph

Abstract Injections of H3-methionine and H3-leucine were combined with radiochemical and radioautographic technics to study the availability time of H3-methionine and the protein synthetic ability of rat lymphocytes in vivo. Although 98.5 per cent of H3-methionine was removed from the serum 5 minutes after injection, sufficient quantities persisted and/or re-entered the serum from tissues to cause increasing grain counts in radioautographs of large lymphocytes for 1 hour after isotope administration. A small amount of additional labeling occurred during the 2nd hour, but it is calculated that labeling is 97-98 per cent complete by 1 hour. All of the large and medium lymphocytes were labeled in the thymus, lymph node, and thoracic duct lymph at short intervals after injection of 4 µc./Gm. body weight of H3-methionine. Evidence is presented that protein synthesis occurs in the nucleus as well as in the cytoplasm and that newly formed protein is equally distributed between daughter cells following mitosis. Previous immunochemical studies are combined with information on generation time and disappearance rates of radioactivity to suggest that large and medium lymphocytes are constantly producing and releasing proteins. Large and medium cells in lymph and lymph node are more active in this than are similar cells in the thymus. Evidence of reutilization of labeled metabolites in the lymph node and especially in the thymus is discussed. Although not all small lymphocytes were labeled by 4 µc./Gm. body weight of H3-methionine, it was shown that larger doses of isotope would label 100 per cent of them. Small lymphocytes in thoracic duct lymph evidenced significant turnover of labeled protein during the 1st day after isotope administration.

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