Generation and Selection of Rabbit Antibody Libraries by Phage Display

2008 ◽  
pp. 101-128 ◽  
Author(s):  
Christoph Rader
Keyword(s):  
Phage Display ◽  
Rabbit Antibody ◽  
Antibody Libraries ◽  
Selection Of

Combination of ribosome and phage display for fast selection of high affinity VHH antibody fragments

2019 ◽  
pp. 27-33
Author(s):  
YuE Kravchenko ◽  
SV Ivanov ◽  
DS Kravchenko ◽  
EI Frolova ◽  
SP Chumakov
Keyword(s):  
Phage Display ◽  
Selection Procedure ◽  
Free System ◽  
Phage Displays ◽  
High Affinity ◽  
Binding Domains ◽  
A Cell ◽  
Vhh Antibodies ◽  
Efficiency Of Selection ◽  
Selection Of

Selection of antibodies using phage display involves the preliminary cloning of the repertoire of sequences encoding antigen-binding domains into phagemid, which is considered the bottleneck of the method, limiting the resulting diversity of libraries and leading to the loss of poorly represented variants before the start of the selection procedure. Selection in cell-free conditions using a ribosomal display is devoid from this drawback, however is highly sensitive to PCR artifacts and the RNase contamination. The aim of the study was to test the efficiency of a combination of both methods, including pre-selection in a cell-free system to enrich the source library, followed by cloning and final selection using phage display. This approach may eliminate the shortcomings of each method and increase the efficiency of selection. For selection, alpaca VHH antibody sequences suitable for building an immune library were used due to the lack of VL domains. Analysis of immune libraries from the genes of the VH3, VHH3 and VH4 families showed that the VHH antibodies share in the VH3 and VH4 gene groups is insignificant, and selection from the combined library is less effective than from the VHH3 family of sequences. We found that the combination of ribosomal and phage displays leads to a higher enrichment of high-affinity fragments and avoids the loss of the original diversity during cloning. The combined method allowed us to obtain a greater number of different high-affinity sequences, and all the tested VHH fragments were able to specifically recognize the target, including the total protein extracts of cell cultures.

Selection of stably folded proteins by phage-display with proteolysis

2004 ◽  
Vol 271 (9) ◽  
pp. 1609-1614 ◽  
Author(s):  
Yawen Bai ◽  
Hanqiao Feng
Keyword(s):  
Phage Display ◽  
Folded Proteins ◽  
Selection Of

scholarly journals Selection of antibody single-chain variable fragments with improved carbohydrate binding by phage display

1994 ◽  
Vol 269 (13) ◽  
pp. 9533-9538
Author(s):  
S.J. Deng ◽  
C.R. MacKenzie ◽  
J. Sadowska ◽  
J. Michniewicz ◽  
N.M. Young ◽  
...  
Keyword(s):  
Phage Display ◽  
Carbohydrate Binding ◽  
Single Chain ◽  
Single Chain Variable Fragments ◽  
Selection Of

Phage display antibody libraries: A robust approach for generation of recombinant human monoclonal antibodies

2019 ◽  
Vol 135 ◽  
pp. 907-918 ◽  
Author(s):  
Rajesh Kumar ◽  
Hilal Ahmed Parray ◽  
Tripti Shrivastava ◽  
Subrata Sinha ◽  
Kalpana Luthra
Keyword(s):  
Monoclonal Antibodies ◽  
Phage Display ◽  
Human Monoclonal Antibodies ◽  
Robust Approach ◽  
Antibody Libraries ◽  
Phage Display Antibody

scholarly journals Expanding the Versatility of Phage Display II: Improved Affinity Selection of Folded Domains on Protein VII and IX of the Filamentous Phage

PLoS ONE ◽  
2011 ◽  
Vol 6 (2) ◽  
pp. e17433 ◽  
Author(s):  
Geir Åge Løset ◽  
Norbert Roos ◽  
Bjarne Bogen ◽  
Inger Sandlie
Keyword(s):  
Phage Display ◽  
Filamentous Phage ◽  
Affinity Selection ◽  
Selection Of

scholarly journals Selection of Peptides Targeting Helix 31 of Bacterial 16S Ribosomal RNA by Screening M13 Phage-Display Libraries

Molecules ◽  
2011 ◽  
Vol 16 (2) ◽  
pp. 1211-1239 ◽  
Author(s):  
Tek N. Lamichhane ◽  
N. Dinuka Abeydeera ◽  
Anne-Cécile E. Duc ◽  
Philip R. Cunningham ◽  
Christine S. Chow
Keyword(s):  
Phage Display ◽  
Ribosomal Rna ◽  
16S Ribosomal Rna ◽  
Phage Display Libraries ◽  
M13 Phage ◽  
Selection Of

Physical elution in phage display selection of inorganic-binding peptides

2009 ◽  
Vol 29 (1) ◽  
pp. 14-19 ◽  
Author(s):  
Senem Donatan ◽  
Hilal Yazici ◽  
Hakan Bermek ◽  
Mehmet Sarikaya ◽  
Candan Tamerler ◽  
...  
Keyword(s):  
Phage Display ◽  
Binding Peptides ◽  
Selection Of
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