Occurrence and Roles of the Obligate Hydrocarbonoclastic Bacteria in the Ocean When There Is No Obvious Hydrocarbon Contamination

2019 ◽  
pp. 337-352 ◽  
Author(s):  
Tony Gutierrez
Keyword(s):  
Hydrocarbon Contamination ◽  
Hydrocarbonoclastic Bacteria

Corrections for surface films in microanalysis by EDS and EELS

1992 ◽  
Vol 50 (2) ◽  
pp. 1230-1231
Author(s):  
J. Bentley ◽  
E. A. Kenik
Keyword(s):  
Hydrocarbon Contamination ◽  
Specimen Preparation ◽  
Surface Films ◽  
Ion Milling ◽  
Preferential Sputtering ◽  
Electron Energy Loss Spectrometry ◽  
Composition Determination ◽  
Analytical Electron ◽  
First Order Correction ◽  
Electron Energy Loss

Common artifacts on analytical electron microscope (AEM) specimens prepared from bulk materials are surface films with altered structure and composition that result from electropolishing, oxidation, hydrocarbon contamination, or ion milling (preferential sputtering or deposition of sputtered specimen or support material). Of course, the best solution for surface films is to avoid them by improved specimen preparation and handling procedures or to remove them by low energy ion sputter cleaning, a capability that already exists on some specialized AEMs and one that is likely to become increasingly common. However, the problem remains and it is surprising that surface films have not received more attention with respect to composition determination by energy dispersive X-ray spectrometry (EDS) and electron energy loss spectrometry (EELS).For EDS, an effective first-order correction to remove the contribution of surface films on wedge shaped specimens is to subtract from the spectrum of interest a spectrum obtained under identical conditions (probe current, diffracting conditions, acquisition live time) from a thinner region of the specimen.

scholarly journals Effects of Dispersants and Biosurfactants on Crude-Oil Biodegradation and Bacterial Community Succession

2021 ◽  
Vol 9 (6) ◽  
pp. 1200
Author(s):  
Gareth E. Thomas ◽  
Jan L. Brant ◽  
Pablo Campo ◽  
Dave R. Clark ◽  
Frederic Coulon ◽  
...  
Keyword(s):  
Crude Oil ◽  
Early Stage ◽  
Niche Partitioning ◽  
Hydrocarbon Biodegradation ◽  
Hydrocarbonoclastic Bacteria ◽  
Bacterial Response ◽  
Oil Biodegradation ◽  
Interfacial Surface ◽  
Degrading Bacteria ◽  
Oil Spill Response

This study evaluated the effects of three commercial dispersants (Finasol OSR 52, Slickgone NS, Superdispersant 25) and three biosurfactants (rhamnolipid, trehalolipid, sophorolipid) in crude-oil seawater microcosms. We analysed the crucial early bacterial response (1 and 3 days). In contrast, most analyses miss this key period and instead focus on later time points after oil and dispersant addition. By focusing on the early stage, we show that dispersants and biosurfactants, which reduce the interfacial surface tension of oil and water, significantly increase the abundance of hydrocarbon-degrading bacteria, and the rate of hydrocarbon biodegradation, within 24 h. A succession of obligate hydrocarbonoclastic bacteria (OHCB), driven by metabolite niche partitioning, is demonstrated. Importantly, this succession has revealed how the OHCB Oleispira, hitherto considered to be a psychrophile, can dominate in the early stages of oil-spill response (1 and 3 days), outcompeting all other OHCB, at the relatively high temperature of 16 °C. Additionally, we demonstrate how some dispersants or biosurfactants can select for specific bacterial genera, especially the biosurfactant rhamnolipid, which appears to provide an advantageous compatibility with Pseudomonas, a genus in which some species synthesize rhamnolipid in the presence of hydrocarbons.

Pilot-Scale Demonstration of DARAMEND Enhanced Bioremediation of Sediment Contaminated with Polycyclic Aromatic Hydrocarbons in Hamilton Harbour

1996 ◽  
Vol 31 (3) ◽  
pp. 433-452 ◽  
Author(s):  
Paul Bucens ◽  
Alan Seech ◽  
Igor Marvan
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Pilot Scale ◽  
Hydrocarbon Contamination ◽  
Active Management ◽  
Treatment Technology ◽  
Technology Program ◽  
Hamilton Harbour ◽  
Polycyclic Aromatic ◽  
Enhanced Bioremediation

Abstract In 1992, GRACE Dearborn, Inc. was contracted by Environment Canada to conduct a field-scale demonstration of the DARAMEND bioremediation technology under the auspices of the Great Lakes Cleanup Fund's Contaminated Sediment Treatment Technology Program. The demonstration on approximately 150 tonnes of sediment dredged from Hamilton Harbour was completed by January 1994. Two distinct batches of sediment were treated. One batch of 90 tonnes of sediment, dredged directly from the harbour without any pretreatment, is the focus of this paper. Sediment was received in October 1992 and was treated through to December 1993. Treatment was conducted in an high-density polyethylene-lined treatment cell that was covered by a steel framed greenhouse. The treatment involved amending, tilling and closely controlling the sediment water content. In approximately 300 days of treatment, the level of total polycyclic aromatic hydrocarbon contamination was reduced from approximately 1,000 µg/g to 100 µg/g, corresponding to a destruction and removal efficiency of approximately 90%. Notably, the high molecular weight polycyclic aromatic hydrocarbons (containing 4 to 6 benzene rings) were effectively degraded to concentrations below the Ministry of the Environment and Energy’s Soil Placement Guideline for controlled fill (MOEE 1992). The trend in the data suggest that following addition of DARAMEND amendment and several months of active management, the polycyclic aromatic hydrocarbons would continue to biodegrade as a result of the DARAMEND amendment even without active management of the sediment.

scholarly journals Characterization of microbial response to petroleum hydrocarbon contamination in a lacustrine ecosystem

2021 ◽  
Author(s):  
Emilio D’Ugo ◽  
Milena Bruno ◽  
Arghya Mukherjee ◽  
Dhrubajyoti Chattopadhyay ◽  
Roberto Giuseppetti ◽  
...  
Keyword(s):  
Oil Spill ◽  
Petroleum Hydrocarbon ◽  
Oil Spills ◽  
Oil Pollution ◽  
Primary Source ◽  
Hydrocarbon Contamination ◽  
Petroleum Hydrocarbon Contamination ◽  
Microbial Response ◽  
Pollution Event ◽  
Hydrocarbonoclastic Potential

AbstractMicrobiomes of freshwater basins intended for human use remain poorly studied, with very little known about the microbial response to in situ oil spills. Lake Pertusillo is an artificial freshwater reservoir in Basilicata, Italy, and serves as the primary source of drinking water for more than one and a half million people in the region. Notably, it is located in close proximity to one of the largest oil extraction plants in Europe. The lake suffered a major oil spill in 2017, where approximately 400 tons of crude oil spilled into the lake; importantly, the pollution event provided a rare opportunity to study how the lacustrine microbiome responds to petroleum hydrocarbon contamination. Water samples were collected from Lake Pertusillo 10 months prior to and 3 months after the accident. The presence of hydrocarbons was verified and the taxonomic and functional aspects of the lake microbiome were assessed. The analysis revealed specialized successional patterns of lake microbial communities that were potentially capable of degrading complex, recalcitrant hydrocarbons, including aromatic, chloroaromatic, nitroaromatic, and sulfur containing aromatic hydrocarbons. Our findings indicated that changes in the freshwater microbial community were associated with the oil pollution event, where microbial patterns identified in the lacustrine microbiome 3 months after the oil spill were representative of its hydrocarbonoclastic potential and may serve as effective proxies for lacustrine oil pollution.

scholarly journals ENVIROMENTAL HYDROCARBON CONTAMINATION IN RICOTTA AND MOZZARELLA DI BUFALA CHEESE

2010 ◽  
Vol 1 (8) ◽  
pp. 45 ◽  
Author(s):  
R Mercogliano ◽  
A De Felice ◽  
C Chirollo ◽  
R Marrone ◽  
M Panzardi ◽  
...  
Keyword(s):  
Hydrocarbon Contamination

The Bioremediation Potential of Hydrocarbonoclastic Bacteria Isolated From a Mangrove Contaminated by Petroleum Hydrocarbons on the Cilacap Coast, Indonesia

2013 ◽  
Vol 17 (1) ◽  
pp. 11-20 ◽  
Author(s):  
Agung Dhamar Syakti ◽  
Mohamad Yani ◽  
Nuning Vita Hidayati ◽  
Asrul Sahri Siregar ◽  
Pierre Doumenq ◽  
...  
Keyword(s):  
Petroleum Hydrocarbons ◽  
Hydrocarbonoclastic Bacteria

Hydrocarbon Contamination in Sediments of Nueces Bay, Texas

2000 ◽  
Vol 65 (2) ◽  
pp. 253-260 ◽  
Author(s):  
V. K. Sharma ◽  
S. D. Hicks ◽  
W. Rivera ◽  
F. G. Vazquez
Keyword(s):  
Hydrocarbon Contamination ◽  
Nueces Bay
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