Effect of Oral Biofilms on Dental Materials: Biocorrosion and Biodeterioration

2021 ◽  
pp. 85-97
Author(s):  
Ivana Nedeljkovic
Keyword(s):  
Dental Materials ◽  
Oral Biofilms

scholarly journals Human In Situ Study of the effect of Bis(2-Methacryloyloxyethyl) Dimethylammonium Bromide Immobilized in Dental Composite on Controlling Mature Cariogenic Biofilm

2018 ◽  
Vol 19 (11) ◽  
pp. 3443 ◽  
Author(s):  
Mary Melo ◽  
Michael Weir ◽  
Vanara Passos ◽  
Juliana Rolim ◽  
Christopher Lynch ◽  
...  
Keyword(s):  
Dental Caries ◽  
Dental Materials ◽  
Significant Inhibition ◽  
Dental Composite ◽  
Antibiofilm Activity ◽  
Dental Resin ◽  
Biofilm Growth ◽  
Restorative Treatment ◽  
Oral Biofilms

Cariogenic oral biofilms cause recurrent dental caries around composite restorations, resulting in unprosperous oral health and expensive restorative treatment. Quaternary ammonium monomers that can be copolymerized with dental resin systems have been explored for the modulation of dental plaque biofilm growth over dental composite surfaces. Here, for the first time, we investigated the effect of bis(2-methacryloyloxyethyl) dimethylammonium bromide (QADM) on human overlying mature oral biofilms grown intra-orally in human participants for 7–14 days. Seventeen volunteers wore palatal devices containing composite specimens containing 10% by mass of QADM or a control composite without QADM. After 7 and 14 days, the adherent biofilms were collected to determine bacterial counts via colony-forming unit (CFU) counts. Biofilm viability, chronological changes, and percentage coverage were also determined through live/dead staining. QADM composites caused a significant inhibition of Streptococcus mutans biofilm formation for up to seven days. No difference in the CFU values were found for the 14-day period. Our findings suggest that: (1) QADM composites were successful in inhibiting 1–3-day biofilms in the oral environment in vivo; (2) QADM significantly reduced the portion of the S. mutans group; and (3) stronger antibiofilm activity is required for the control of mature long-term cariogenic biofilms. Contact-killing strategies using dental materials aimed at preventing or at least reducing high numbers of cariogenic bacteria seem to be a promising approach in patients at high risk of the recurrence of dental caries around composites.

scholarly journals A Novel Dental Sealant Containing Dimethylaminohexadecyl Methacrylate Suppresses the Cariogenic Pathogenicity of Streptococcus mutans Biofilms

2019 ◽  
Vol 20 (14) ◽  
pp. 3491 ◽  
Author(s):  
Maria Salem Ibrahim ◽  
Ahmed S. Ibrahim ◽  
Abdulrahman A. Balhaddad ◽  
Michael D. Weir ◽  
Nancy J. Lin ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Dental Materials ◽  
Acid Stress ◽  
Dental Resin ◽  
Biofilm Inhibition ◽  
Biofilm Growth ◽  
Dental Sealants ◽  
Oral Biofilms ◽  
Caries Management ◽  
Management Approaches

Cariogenic oral biofilms are strongly linked to dental caries around dental sealants. Quaternary ammonium monomers copolymerized with dental resin systems have been increasingly explored for modulation of biofilm growth. Here, we investigated the effect of dimethylaminohexadecyl methacrylate (DMAHDM) on the cariogenic pathogenicity of Streptococcus mutans (S. mutans) biofilms. DMAHDM at 5 mass% was incorporated into a parental formulation containing 20 mass% nanoparticles of amorphous calcium phosphate (NACP). S. mutans biofilms were grown on the formulations, and biofilm inhibition and virulence properties were assessed. The tolerances to acid stress and hydrogen peroxide stress were also evaluated. Our findings suggest that incorporating 5% DMAHDM into 20% NACP-containing sealants (1) imparts a detrimental biological effect on S. mutans by reducing colony-forming unit counts, metabolic activity and exopolysaccharide synthesis; and (2) reduces overall acid production and tolerance to oxygen stress, two major virulence factors of this microorganism. These results provide a perspective on the value of integrating bioactive restorative materials with traditional caries management approaches in clinical practice. Contact-killing strategies via dental materials aiming to prevent or at least reduce high numbers of cariogenic bacteria may be a promising approach to decrease caries in patients at high risk.

scholarly journals Bio-Interactive Zwitterionic Dental Biomaterials for Improving Biofilm Resistance: Characteristics and Applications

2020 ◽  
Vol 21 (23) ◽  
pp. 9087
Author(s):  
Utkarsh Mangal ◽  
Jae-Sung Kwon ◽  
Sung-Hwan Choi
Keyword(s):  
Biofilm Formation ◽  
Periodontal Diseases ◽  
Dental Materials ◽  
Causative Factor ◽  
Oral Biofilm ◽  
Zwitterionic Polymers ◽  
Oral Biofilms ◽  
Protein Adhesion ◽  
Microbial Environment ◽  
Significant Attention

Biofilms are formed on surfaces inside the oral cavity covered by the acquired pellicle and develop into a complex, dynamic, microbial environment. Oral biofilm is a causative factor of dental and periodontal diseases. Accordingly, novel materials that can resist biofilm formation have attracted significant attention. Zwitterionic polymers (ZPs) have unique features that resist protein adhesion and prevent biofilm formation while maintaining biocompatibility. Recent literature has reflected a rapid increase in the application of ZPs as coatings and additives with promising outcomes. In this review, we briefly introduce ZPs and their mechanism of antifouling action, properties of human oral biofilms, and present trends in anti-biofouling, zwitterionic, dental materials. Furthermore, we highlight the existing challenges in the standardization of biofilm research and the future of antifouling, zwitterated, dental materials.

Intensive course in dental materials for teachers of dental auxiliaries

1973 ◽  
Vol 37 (2) ◽  
pp. 39-42
Author(s):  
JB Moser ◽  
EP Lautenschlager ◽  
BK Moore ◽  
EH Greener
Keyword(s):  
Dental Materials ◽  
Intensive Course ◽  
Dental Auxiliaries

Microbiological purity of syringes containing composites in the context of cross-infection prevention in dental practices

2020 ◽  
Vol 33 (2) ◽  
pp. 102-105
Author(s):  
Joanna Bialowska ◽  
Witold Bojar ◽  
Tomasz Zareba ◽  
Stefan Tyski ◽  
Barbara Tymczyna-Borowicz
Keyword(s):  
Infection Prevention ◽  
Dental Materials ◽  
Cross Infection ◽  
Aerobic Bacteria ◽  
Filling Material ◽  
Bodily Fluids ◽  
Dental Office ◽  
Dental Practices ◽  
Dental Offices ◽  
Aerobic And Anaerobic

AbstractCross-infection involves the transmission of microorganisms through secretions, bodily fluids and excreta, as well as undisinfected surfaces and medical equipment. In the dental office, diseases are transmitted via various routes, e.g. from patient to dentist or other member of dental team, from doctor or dental team member to patient, from patient to another patient, from dental office to community and from community to patient. The study was conducted to evaluate the effectiveness of infection control in dental practices based on the qualitative and quantitative assessment of microbiological contaminants detected on the surface of filling material packaging used in dental offices. The material for research were 9 packages containing dental materials during their use in 3 dental settings. The packages were placed in sterile flasks and rinsed to wash microorganisms from the surfaces. The washes were filtered through membrane filters and cultured under proper aerobic and anaerobic conditions, and at elevated CO2 concentration. Microbial growth on TIO and TSB media was observed. The contamination of most samples remained low as indicated by the growth from one to a maximum of five colonies on TSA. The contamination remained at the level of 10-50 CFU/package, i.e. <100 CFU/single package. The tests evaluating the contamination of dental package surfaces with aerobic bacteria confirmed high hygiene standards observed in dental offices from which the packages were brought.

Cultivated and not-yet-cultivated bacteria in oral biofilms

2009 ◽  
Vol 21 (2) ◽  
Author(s):  
Ingar Olsen ◽  
Dorita Preza ◽  
Jørn A. Aas ◽  
Bruce J. Paster
Keyword(s):  
Oral Biofilms
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