Aim:
Apart from the modifiable risk factors, genetic factors are believed to also influence the outcome of the
coronary artery diseases (CAD). Under the genetic factors, miRNA polymorphisms, namely Hsa-miR-146a-5p
(rs2910164) have become an important tool to study the mechanism that underlies the pathogenesis of this disease.
Therefore, we investigated the association of miR-146a gene variations with susceptibility of coronary artery diseases.
Methodology: This study was conducted on 100 CAD patients and 117 matched healthy individuals. Genotyping of the
Hsa-miR-146a-5p C>G gene variation was performed by using amplification refractory mutation system PCR method
(ARMS-PCR).
Results:
The distribution of Hsa-miR-146a-5p rs2910164 C>G genotypes observed between patients and controls was
significantly different (P=0.048). Moreover, the frequency of G allele (fG) was found to be significantly higher among
patients than in controls (0.36 vs. 0.25). Our findings showed that the Hsa-miR-146a-5p C>G variant was associated with
an increased risk of CAD in codominant inheritance model CC vs. CG genotype (OR = 1.84, 95 % CI, 1.02-3.31;
p=0.040) and (OR = 3.18, 95 % CI, 1.02-9.9; p=0.045) for CC vs. GG genotype in dominant inheritance model. Whereas
the G allele significantly increased the risk of coronary artery disease (OR =1,81, 95 % CI, 1.18-2.78; p=0.006) compared
to C allele. Taken together, these results demonstrated that miR-146a/rs2910164 is associated with susceptibility to
coronary artery disease, providing novel insights into the genetic etiology and underlying biology of coronary artery
disease.
Conclusion:
Our findings indicated that Hsa-miR-146a-5p rs2910164 GG genotype and G allele are associated with an
increased susceptibility to Coronary Artery Disease. A larger sample size can be the key to progress in establishing
the genetic co-relation of miRNA gene polymorphisms and cardiovascular diseases.
