Electrochemical Methods Applied for Bioanalysis: Differential Pulse Voltammetry and Square Wave Voltammetry

Square wave voltammetry and differential pulse voltammetry have been used for the determination of 2-acetamidofluorene and fluoren-9-ol using carbon paste electrodes, following the study of the influence of the carbon paste composition on the voltammetric signals of the analytes. The methods are based on the oxidation of the above compounds and they include adsorptive accumulation of the analyte on the surface of the working electrode. The limit of detection was 1 μmol l-1for fluoren-9-ol in a medium of 0.1 M H2SO4, and 40 nmol l-1for 2-acetamidofluorene in Britton-Robinson buffer (pH 7).

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Voltammetric determination of ciprofloxacin in urine samples and its interaction with dsDNA on a cathodically pretreated boron-doped diamond electrode

Analytical Methods ◽

10.1039/c5ay00625b ◽

2015 ◽

Vol 7 (8) ◽

pp. 3411-3418 ◽

Cited By ~ 25

Author(s):

Gustavo Stoppa Garbellini ◽

Romeu C. Rocha-Filho ◽

Orlando Fatibello-Filho

Keyword(s):

Differential Pulse Voltammetry ◽

Square Wave Voltammetry ◽

Differential Pulse ◽

Boron Doped Diamond ◽

Diamond Electrode ◽

Boron Doped ◽

Wave Voltammetry ◽

Boron Doped Diamond Electrode ◽

Pulse Voltammetry

A cathodically pretreated boron-doped diamond electrode is successfully used to determine ciprofloxacin (CIP) by differential pulse voltammetry and to infer the type of binding of CIP to DNA by square-wave voltammetry.

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Pulse voltammetric methods of analysis

Philosophical Transactions of the Royal Society of London. Series A, Mathematical and Physical Sciences ◽

10.1098/rsta.1981.0170 ◽

1981 ◽

Vol 302 (1468) ◽

pp. 315-326 ◽

Cited By ~ 24

Keyword(s):

Differential Pulse Voltammetry ◽

High Performance ◽

Square Wave Voltammetry ◽

Differential Pulse ◽

Pulse Technique ◽

Wave Voltammetry ◽

Rapid Pulse ◽

Pulse Voltammetry ◽

Voltammetric Methods

The techniques of normal and differential pulse voltammetry are presented together with equations describing these techniques. A number of specific applications for both differential and normal pulse, including determination of As III in sewage and the determination of sulphide, are presented. Advantages of amperometric titrations in eliminating ‘background’ errors are shown, with the titration of Cu II with EDTA as a specific example. The use of square-wave voltammetry, a new, rapid-pulse technique, is presented, and an application in which this technique is employed in an electrochemical high performance chromatographic detector used for nitrosamine analysis is discussed.

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Potassium Ferro cyanide electrochemically detected by Differential Pulse and Square Wave Voltammetry in a Competition using Gradient Boosting as Machine Learning Algorithm

2020 4th International Symposium on Multidisciplinary Studies and Innovative Technologies (ISMSIT) ◽

10.1109/ismsit50672.2020.9254514 ◽

2020 ◽

Author(s):

Nemah Abu Shama ◽

Suleyman Asir ◽

Kamil Dimililer ◽

Mehmet Ozsoz

Keyword(s):

Machine Learning ◽

Square Wave Voltammetry ◽

Learning Algorithm ◽

Differential Pulse ◽

Gradient Boosting ◽

Machine Learning Algorithm ◽

Square Wave ◽

Wave Voltammetry

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Exploring the Interaction of Ethidium Bromide and HOECHST 33258 with DNA by Means of Electrochemical Approach

Biophysical Reviews and Letters ◽

10.1142/s1793048017500084 ◽

2017 ◽

Vol 12 (03) ◽

pp. 151-161

Author(s):

Levon A. Tavadyan ◽

Seyran H. Minasyan ◽

Gaspar H. Kocharyan ◽

Ara P. Antonyan ◽

Viktoria G. Sahakyan ◽

...

Keyword(s):

Ethidium Bromide ◽

Square Wave Voltammetry ◽

Differential Pulse ◽

Binding Modes ◽

Hoechst 33258 ◽

Wave Voltammetry ◽

Calf Thymus ◽

Electrochemical Approach ◽

Pulse Voltammetry ◽

Binding Type

The binding of both ethidium bromide (EtBr) and Hoechst 33258 (H33258) to calf thymus DNA differing by the mechanism at different ionic strengths of NaCl water solutions (2, 20 and 150[Formula: see text]mM) has been quantitatively studied by square wave voltammetry (SWV). It was revealed that EtBr binds to DNA by more than one mode: at the solution ionic strengths 2 and 150[Formula: see text]mM, two modes were revealed, at 20[Formula: see text]mM — three modes. Values of EtBr binding constant ([Formula: see text]) to DNA and the number of nucleotides per one binding site ([Formula: see text]) for respective binding modes were determined. For H33258 under the same conditions, two binding modes were found and for both these modes values of [Formula: see text] and [Formula: see text] were determined. Dependence of the binding types and parameters on the solution ionic strength by SWV method was revealed, which differs by the binding type of EtBr and H33258. The obtained data are in good accordance with the ones determined by spectroscopic (absorption and fluorescence) and differential pulse voltammetry (DPV) methods. The obtained data indicate the sensibility and high precision of SWV method which may be applied to studies on interaction of different ligands with DNA, which reveal some peculiarities of their binding to DNA that are hidden and are not found by other methods.

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