A Hypothetical Explanation of Anomalous Polarographic Behaviour of Protein Solutions

1971 ◽  
pp. 153-156 ◽  
Author(s):  
M. Baumann
Keyword(s):  
Protein Solutions ◽  
Polarographic Behaviour ◽  
Hypothetical Explanation

Characterization of frozen hydrated biological specimens by low-loss EELS

1992 ◽  
Vol 50 (2) ◽  
pp. 1572-1573
Author(s):  
Songquan Sun ◽  
Richard D. Leapman
Keyword(s):  
Water Content ◽  
Direct Method ◽  
Internal Standard ◽  
Dry Weight ◽  
Dark Field ◽  
Protein Solutions ◽  
Subcellular Compartment ◽  
Differential Shrinkage ◽  
Electron Energy Loss

Analyses of ultrathin cryosections are generally performed after freeze-drying because the presence of water renders the specimens highly susceptible to radiation damage. The water content of a subcellular compartment is an important quantity that must be known, for example, to convert the dry weight concentrations of ions to the physiologically more relevant molar concentrations. Water content can be determined indirectly from dark-field mass measurements provided that there is no differential shrinkage between compartments and that there exists a suitable internal standard. The potential advantage of a more direct method for measuring water has led us to explore the use of electron energy loss spectroscopy (EELS) for characterizing biological specimens in their frozen hydrated state.We have obtained preliminary EELS measurements from pure amorphous ice and from cryosectioned frozen protein solutions. The specimens were cryotransfered into a VG-HB501 field-emission STEM equipped with a 666 Gatan parallel-detection spectrometer and analyzed at approximately −160 C.

Polarographic Determination of 6-β-D-Glucopyranosyloxy-7-hydroxycoumarin

1996 ◽  
Vol 61 (3) ◽  
pp. 333-341
Author(s):  
Jiří Barek ◽  
Roman Hrnčíř ◽  
Josino C. Moreira ◽  
Jiří Zima
Keyword(s):  
Natural Compound ◽  
Mercury Electrode ◽  
Direct Determination ◽  
Differential Pulse ◽  
Differential Pulse Polarography ◽  
Whitening Agent ◽  
Polarographic Behaviour ◽  
Pulse Polarography ◽  
Dropping Mercury Electrode

The polarographic behaviour was studied for 6-β-D-glucopyranosyloxy-7-hydroxycoumarin, a natural compound serving as an optical whitening agent. The substance can be quantitated by tast polarography, differential pulse polarography using a conventional dropping mercury electrode, and differential pulse polarography using a static mercury drop electrode over the regions of 20-1 000, 2-1 000, and 0.2-1 000 μmol l-1, respectively. The methods developed for the quantitation of the compound were applied to its direct determination in a raw product.

scholarly journals THE REFRACTIVITY OF PROTEIN SOLUTIONS

1935 ◽  
Vol 108 (3) ◽  
pp. 703-707
Author(s):  
David B. Hand
Keyword(s):  
Protein Solutions

scholarly journals The Different Colors of mAbs in Solution

Antibodies ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 21
Author(s):  
Alexandre Ambrogelly
Keyword(s):  
Visual Inspection ◽  
Quantitative Methods ◽  
Geographical Area ◽  
Therapeutic Protein ◽  
Protein Solutions ◽  
Protein Solution ◽  
Critical Quality Attribute ◽  
Recombinant Therapeutic Protein ◽  
Protein Variants ◽  
Antibody Solution

The color of a therapeutic monoclonal antibody solution is a critical quality attribute. Consistency of color is typically assessed at time of release and during stability studies against preset criteria for late stage clinical and commercial products. A therapeutic protein solution’s color may be determined by visual inspection or by more quantitative methods as per the different geographical area compendia. The nature and intensity of the color of a therapeutic protein solution is typically determined relative to calibrated standards. This review covers the analytical methodologies used for determining the color of a protein solution and presents an overview of protein variants and impurities known to contribute to colored recombinant therapeutic protein solutions.

scholarly journals How can electric lighting contribute to human health and well-being?

2021 ◽  
Vol 53 (5) ◽  
pp. 515-522
Author(s):  
P Raynham
Keyword(s):  
Human Health ◽  
Psychological Impact ◽  
Health Benefits ◽  
Well Being ◽  
Health Problems ◽  
Starting Point ◽  
Electric Light ◽  
Health And Well Being ◽  
Hypothetical Explanation

Electric light in buildings may provide some health benefits; however, for most people these benefits are likely to be small. It is possible for electric lighting to cause health problems, if there is too little light or there is glare, but for the most part there is good guidance available and these problems can be avoided. The quality of the lit environment can have a psychological impact and this may in turn impact well-being. A starting point for this is perceived adequacy of illumination. Related lighting metrics are examined and a hypothetical explanation is suggested.

scholarly journals The crystallization enthalpy and entropy of protein solutions: microcalorimetry, van't Hoff determination and linearized Poisson–Boltzmann model of tetragonal lysozyme crystals

2021 ◽  
Vol 23 (4) ◽  
pp. 2686-2696
Author(s):  
Lorena Hentschel ◽  
Jan Hansen ◽  
Stefan U. Egelhaaf ◽  
Florian Platten
Keyword(s):  
Theoretical Description ◽  
Protein Solutions ◽  
Consistent Picture ◽  
Poisson Boltzmann ◽  
Enthalpy And Entropy ◽  
Boltzmann Model ◽  
Lysozyme Crystals ◽  
Solution Parameters

Microcalorimetric and van't Hoff determinations as well as a theoretical description provide a consistent picture of the crystallization enthalpy and entropy of protein solutions and their dependence on physicochemical solution parameters.

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