High-resolution two-dimensional gel electrophoresis and protein identification using western blotting and ECL detection

2000 ◽  
pp. 55-67
Author(s):  
Julio E. Celis ◽  
Pavel Gromov
Keyword(s):  
High Resolution ◽  
Western Blotting ◽  
Gel Electrophoresis ◽  
Protein Identification ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis

Diversity of Glycoprotein Deficiencies in Glanzmann’s Thrombasthenia

1985 ◽  
Vol 54 (03) ◽  
pp. 626-629 ◽  
Author(s):  
M Meyer ◽  
F H Herrmann
Keyword(s):  
High Resolution ◽  
Gel Electrophoresis ◽  
Type Ii ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Structural Variant ◽  
Glanzmann’S Thrombasthenia ◽  
Crossed Immunoelectrophoresis ◽  
Glanzmann's Thrombasthenia ◽  
Platelet Proteins

SummaryThe platelet proteins of 9 thrombasthenic patients from 7 families were analysed by high resolution two-dimensional gel electrophoresis (HR-2DE) and crossed immunoelectrophoresis (CIE). In 7 patients both glycoproteins (GPs) IIb and Ilia were absent or reduced to roughly the same extent. In two related patients only a trace of GP Ilb-IIIa complex was detected in CIE, but HR-2DE revealed a glycopeptide in the position of GP Ilia in an amount comparable to type II thrombasthenia. This GP Ilia-like component was neither recognized normally by anti-GP Ilb-IIIa antibodies nor labeled by surface iodination. In unreduced-reduced two-dimensional gel electrophoresis two components were observed in the region of GP Ilia. The assumption of a structural variant of GP Ilia in the two related patients is discussed.

ACCURATE NONLINEAR REGISTRATION FOR TWO-DIMENSIONAL GEL ELECTROPHORESIS IMAGES

2013 ◽  
Vol 21 (03) ◽  
pp. 1350020
Author(s):  
HUA-MEI XIN ◽  
YUEMIN ZHU
Keyword(s):  
Thin Plate ◽  
Gel Electrophoresis ◽  
Protein Identification ◽  
Distance Measure ◽  
Spline Interpolation ◽  
Thin Plate Spline ◽  
Two Dimensional ◽  
Registration Accuracy ◽  
Two Dimensional Gel Electrophoresis ◽  
Nonlinear Registration

Two-dimensional gel electrophoresis (2DGE) images are an important support for the analysis of proteins in proteomics. The registration of 2DGE images is considered as one of key elements in protein identification while it is a difficult problem. This paper proposes a new accurate nonlinear registration approach for 2DGE images, based on the exploitation of both spot distance measure and spot intensity. The method consists of three steps: multi-resolution affine registration, spot pairing and thin-plate spline interpolation. The results on both simulated and real gel images show that the proposed method significantly improves registration accuracy in comparison with thin-plate spline registration techniques.

Technology: Methodology for High-Resolution Two-Dimensional Gel Electrophoresis

1987 ◽  
Vol 5 (2) ◽  
pp. 127-149 ◽  
Author(s):  
A Thomas Endler ◽  
Donald S. Young ◽  
Russell P. Tracy
Keyword(s):  
High Resolution ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis
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