Microinjection of Macromolecules into Mammalian Cells in Culture

1999 ◽  
pp. 16-30 ◽  
Author(s):  
R. Perona ◽  
F. Dolfi ◽  
J. Feramisco ◽  
J. C. Lacal
Keyword(s):  
Mammalian Cells ◽  
Cells In Culture

Effect of pulsing electromagnetic fields on DNA synthesis in mammalian cells in culture

1986 ◽  
Vol 42 (2) ◽  
pp. 185-186 ◽  
Author(s):  
K. Takahashi ◽  
I. Kaneko ◽  
M. Date ◽  
E. Fukada
Keyword(s):  
Dna Synthesis ◽  
Electromagnetic Fields ◽  
Mammalian Cells ◽  
Cells In Culture

scholarly journals Replication of DNA containing 5-bromouracil can be mutagenic in Syrian hamster cells.

1984 ◽  
Vol 4 (11) ◽  
pp. 2449-2454 ◽  
Author(s):  
E R Kaufman
Keyword(s):  
Syrian Hamster ◽  
Mammalian Cells ◽  
Culture Medium ◽  
Cells In Culture ◽  
Thymidine Analog ◽  
High Concentrations

A new protocol for inducing mutations in mammalian cells in culture by exposure to the thymidine analog 5-bromodeoxyuridine (BrdUrd) was established. This protocol, called "DNA-dependent" mutagenesis, involved the incorporation of BrdUrd into DNA under nonmutagenic conditions and the subsequent replication of the 5-bromouracil (BrUra)-containing DNA under mutagenic conditions but with no BrdUrd present in the culture medium. The mutagenic conditions were induced by allowing BrUra-containing DNA to replicate in the presence of high concentrations of thymidine. This generated high intracellular levels of dTTP and dGTP, causing nucleotide pool imbalance. The mutagenesis induced by this protocol was found to correlate with the level of BrUra substituted for thymine in DNA.

Effects of a trinucleotide ethyl phosphotriester, Gmp(Et)Gmp(Et)U, on mammalian cells in culture

Biochemistry ◽  
1977 ◽  
Vol 16 (9) ◽  
pp. 1988-1996 ◽  
Author(s):  
Paul S. Miller ◽  
Lita T. Braiterman ◽  
Paul O. P. Ts'o
Keyword(s):  
Mammalian Cells ◽  
Cells In Culture

scholarly journals Can ends justify the means?: Telomeres and the mechanisms of replicative senescence and immortalization in mammalian cells

1998 ◽  
Vol 95 (16) ◽  
pp. 9078-9081 ◽  
Author(s):  
John M. Sedivy
Keyword(s):  
Knockout Mice ◽  
Mammalian Cells ◽  
Replicative Senescence ◽  
Somatic Cells ◽  
Focus Attention ◽  
Replicative Lifespan ◽  
Cells In Culture ◽  
Lively Debate

Finite replicative lifespan, or senescence, of mammalian cells in culture is a phenomenon that has generated much curiosity since its description. The obvious significance of senescence to organismal aging and the development of cancer has engendered a long-lasting and lively debate about its mechanisms. Recent discoveries concerning the phenotypes of telomerase knockout mice, the consequences of telomerase reexpression in somatic cells, and genes that regulate senescence have provided striking molecular insights but also have uncovered important new questions. The objective of this review is to reconcile old observations with new molecular details and to focus attention on the key remaining puzzles.

Nocodazole and cytochalasin D induce tetraploidy in mammalian cells

1984 ◽  
Vol 246 (1) ◽  
pp. C154-C156 ◽  
Author(s):  
G. W. Zieve
Keyword(s):  
Cell Division ◽  
Mammalian Cells ◽  
Cytochalasin D ◽  
Microtubule Assembly ◽  
Reversible Inhibitor ◽  
Cleavage Furrow ◽  
Synchronized Cells ◽  
Cells In Culture ◽  
Wide Range

Nocodazole, a rapidly reversible inhibitor of microtubule assembly is useful for preparing mammalian cells synchronized at all stages of mitosis. When synchronized cells are allowed to progress through mitosis in the presence of cytochalasin D, the cleavage furrow is inhibited and dikaryon cells are formed. These cells become homogeneous populations of stable mononuclear tetraploid cells after the following cell division. This procedure is applicable to a wide range of mammalian cells in culture.

Proteomic Analysis of Mammalian Cells in Culture

2000 ◽  
Vol 28 (5) ◽  
pp. A230-A230
Author(s):  
Elizabeth Sage ◽  
Carol Marshall ◽  
John Birch ◽  
David James
Keyword(s):  
Proteomic Analysis ◽  
Mammalian Cells ◽  
Cells In Culture
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