Isolation, Identification and Biochemical Characterization of Azospirillum spp. and Other Nitrogen-Fixing Bacteria

The considerable industrial interest in the qualitative and quantitative production of polyhydroxyalkanoates in microorganisms has led to the characterization of those synthesized in the nitrogen-fixing bacteria Azospirillum brasilense and Azotobacter paspali. In contrast to some other bacterial species, Azospirillum brasilense does not produce copolymers of hydroxyalkanoates when grown under the different carbon sources assayed, namely n-alkanoic acids, hydroxyalkanoates, and sugars with varying C:N ratios. Rather, only homopolymers of polyhydroxybutyrate were detected, comprising up to 70% of the cell dry mass. No copolymers were detected in Azotobacter paspali. Quantitative analyses of poly(β-hydroxybutyrate) are also presented.Key words: Azospirillum spp., Azotobacter paspali, polyhydroxyalkanoate analysis, PHA, PHB.

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Characterization of Nitrogen-Fixing Bacteria Containing Molybdenum-Independent Nitrogenases from Diverse Natural Environments

Nitrogen Fixation: From Molecules to Crop Productivity - Current Plant Science and Biotechnology in Agriculture ◽

10.1007/0-306-47615-0_86 ◽

2005 ◽

pp. 179-180

Author(s):

D. Betancourt ◽

T. M. Loveless ◽

P. E. Bishop

Keyword(s):

Natural Environments ◽

Nitrogen Fixing ◽

Nitrogen Fixing Bacteria

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Isolation and characterization of nitrogen-fixing bacteria of the genus Azospirillum from the soil of a Sphagnum peat bog

Microbiology ◽

10.1134/s0026261707010134 ◽

2007 ◽

Vol 76 (1) ◽

pp. 93-101 ◽

Cited By ~ 8

Author(s):

E. V. Doroshenko ◽

E. S. Boulygina ◽

E. M. Spiridonova ◽

T. P. Tourova ◽

I. K. Kravchenko

Keyword(s):

Peat Bog ◽

Nitrogen Fixing ◽

Nitrogen Fixing Bacteria ◽

Sphagnum Peat ◽

Isolation And Characterization

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Amplified ribosomal DNA restriction analysis for the characterization of Azotobacteraceae: a contribution to the study of these free-living nitrogen-fixing bacteria

Journal of Microbiological Methods ◽

10.1016/j.mimet.2004.01.006 ◽

2004 ◽

Vol 57 (2) ◽

pp. 197-206 ◽

Cited By ~ 12

Author(s):

Lucia Aquilanti ◽

Ilaria Mannazzu ◽

Roberto Papa ◽

Lucia Cavalca ◽

Francesca Clementi

Keyword(s):

Ribosomal Dna ◽

Restriction Analysis ◽

Nitrogen Fixing ◽

Nitrogen Fixing Bacteria ◽

Free Living ◽

Dna Restriction

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Enumeration and characterization of nitrogen-fixing bacteria in an eelgrass (Zostera marina) bed

Microbial Ecology ◽

10.1007/bf02075812 ◽

1989 ◽

Vol 18 (3) ◽

pp. 249-259 ◽

Cited By ~ 16

Author(s):

Wung Yang Shieh ◽

Usio Simidu ◽

Yoshiharu Maruyama

Keyword(s):

Zostera Marina ◽

Nitrogen Fixing ◽

Nitrogen Fixing Bacteria

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ISOLATION, MOLECULAR CHARACTERIZATION OF DIAZOTROPIC BACTERIA FROM VIRGIN SOIL FROM ARAKU VALLEY AND ASSESSMENT OF OF AMMONIA ACCUMULATION AT SALINE CONDITIONS

International Journal of Advanced Research ◽

10.21474/ijar01/13809 ◽

2021 ◽

Vol 9 (11) ◽

pp. 824-832

Author(s):

Simmanna Nakka ◽

◽

S. Uday Bhaskar ◽

Keyword(s):

Biochemical Characterization ◽

Growth Parameters ◽

Soil Samples ◽

Virgin Soil ◽

Nitrogen Fixing ◽

Nitrogen Fixing Bacteria ◽

Free Living ◽

Symbiotic Associations ◽

Ammonia Accumulation ◽

Agar Media

Nitrogen-fixing bacteria are widely distributed in nature where they reduce atmospheric nitrogen in soil or in association with plant. They have been found in a wide variety of terrestrial and aquatic habitats in both temperate and tropical regions of the word. Nitrogen-fixing bacteria are found in symbiotic associations with plants free living in soil. The objective of the present research was to isolate free living Nitrogen fixing bacteria from virgin soil samples in araku valley and assessment of their ammonia accumulation at saline conditions. 10 soil samples were collected in different place from virgin areas. For isolation of the free living nitrogen fixing bacteria, Nitrogen free media like Jensens Medium and Azotobacter Agar were used. Serially diluted soil samples were spread on the agar media and incubated for 48 hours. Eleven morphologically different bacteria were separated on made pure colonies on nutrient agar media. All bacteria were under go biochemical characterization which reveals that all these bacteria related to Azospirillum, Azotobacter and Clostridium. High ammonia liberating isolate MGN-10 was molecular characterizes as Azotobacter chroococcum and this soil application increase the plant growth in terms of growth parameters.

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