scholarly journals ISOLATION, MOLECULAR CHARACTERIZATION OF DIAZOTROPIC BACTERIA FROM VIRGIN SOIL FROM ARAKU VALLEY AND ASSESSMENT OF OF AMMONIA ACCUMULATION AT SALINE CONDITIONS

2021 ◽  
Vol 9 (11) ◽  
pp. 824-832
Author(s):  
Simmanna Nakka ◽  
◽  
S. Uday Bhaskar ◽  
Keyword(s):  
Biochemical Characterization ◽  
Growth Parameters ◽  
Soil Samples ◽  
Virgin Soil ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Free Living ◽  
Symbiotic Associations ◽  
Ammonia Accumulation ◽  
Agar Media

Nitrogen-fixing bacteria are widely distributed in nature where they reduce atmospheric nitrogen in soil or in association with plant. They have been found in a wide variety of terrestrial and aquatic habitats in both temperate and tropical regions of the word. Nitrogen-fixing bacteria are found in symbiotic associations with plants free living in soil. The objective of the present research was to isolate free living Nitrogen fixing bacteria from virgin soil samples in araku valley and assessment of their ammonia accumulation at saline conditions. 10 soil samples were collected in different place from virgin areas. For isolation of the free living nitrogen fixing bacteria, Nitrogen free media like Jensens Medium and Azotobacter Agar were used. Serially diluted soil samples were spread on the agar media and incubated for 48 hours. Eleven morphologically different bacteria were separated on made pure colonies on nutrient agar media. All bacteria were under go biochemical characterization which reveals that all these bacteria related to Azospirillum, Azotobacter and Clostridium. High ammonia liberating isolate MGN-10 was molecular characterizes as Azotobacter chroococcum and this soil application increase the plant growth in terms of growth parameters.

scholarly journals Effect of Abandonment on Diversity and Abundance of Free-Living Nitrogen-Fixing Bacteria and Total Bacteria in the Cropland Soils of Hulun Buir, Inner Mongolia

PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e106714 ◽  
Author(s):  
Huhe ◽  
Shinchilelt Borjigin ◽  
Yunxiang Cheng ◽  
Nobukiko Nomura ◽  
Toshiaki Nakajima ◽  
...  
Keyword(s):  
Inner Mongolia ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Free Living ◽  
Total Bacteria

Yield and Rhizosphere - microflora of Cowpea in Response to Magnesium Fertilization in Lateritic Soils of Kerala

2021 ◽  
Author(s):  
V.P. Soniya ◽  
P.S. Bhindhu
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Root Colonization ◽  
Arbuscular Mycorrhizal ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Free Living ◽  
Spore Count ◽  
Lateritic Soils ◽  
Magnesium Uptake

Background: Magnesium deficiency has become a major nutritional disorder in lateritic soils of Kerala. Appropriate magnesium fertilization is the best strategy to combat deficiency issues. Apart from correcting nutritional deficiency, magnesium fertilization has an influence on the growth of beneficial microbes such as nitrogen fixing bacterias and arbuscular mycorrhizal fungi. The experiment aimed to investigate the effect of magnesium fertilization on crop yield and population rhizosphere micoflora of cowpea in lateritic soils of Kerala.Methods: A pot culture experiment was conducted with a gradient of magnesium additions ranging from 5 mg kg-1 to 80 mg kg-1 of soil along with recommended dose of fertilizers. Population of rhizobium, free living nitrogen fixing bacteria, spore count of arbuscular mycorrhizal fungi and per cent root colonization of arbuscular mycorrhizal fungi were studied during flowering. The available magnesium and magnesium uptake were also worked out during harvest. Yield and yield contributing characteristics of cowpea were measured during harvest stage.Result: Magnesium addition produced significant variations in population of rhizobium and free- living nitrogen fixing bacteria whereas spore count of AMF and per cent root colonization of AMF did not vary according to the added doses of magnesium. A higher population of rhizobium, free living nitrogen fixers, root nodules, magnesium uptake, plant height and yield were obtained in the treatment where magnesium was applied @ 10 mg kg-1 soil.

Chemotaxis of free-living nitrogen-fixing bacteria towards maize mucilage

1986 ◽  
pp. 129-139 ◽  
Author(s):  
G. Mandimba ◽  
T. Heulin ◽  
R. Bally ◽  
A. Guckert ◽  
J. Balandreau
Keyword(s):  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Free Living

Rhizosphere nitrogen-fixing bacteria (free-living) contribute to nitrogen absorption in wheat

Rhizosphere ◽  
2020 ◽  
Vol 16 ◽  
pp. 100245
Author(s):  
Jésica Fernanda de Souza Gênero ◽  
Vinícius Rigueiro Messa ◽  
Meirieli Nunes Beladeli ◽  
Antônio Carlos Torres da Costa ◽  
José Barbosa Duarte Júnior
Keyword(s):  
Nitrogen Absorption ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Free Living

scholarly journals Characterization and ecological role of free-living nitrogen-fixing bacteria isolated from the rhizoplane of Melastoma malabathricum inhabiting acidic plain lands in Kalimantan

Tropics ◽  
2006 ◽  
Vol 15 (4) ◽  
pp. 365-369 ◽  
Author(s):  
Yasuyuki HASHIDOKO ◽  
Yukako GOTOU ◽  
Mitsuru OSAKI ◽  
Erry PURNOMO ◽  
Limin H. SUWIDO ◽  
...  
Keyword(s):  
Ecological Role ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Free Living ◽  
Melastoma Malabathricum

Research on Improvement of Nitrogenase Activity of Free-Living Nitrogen-Fixing Bacteria Using DBD Plasma

2013 ◽  
Vol 671-674 ◽  
pp. 2674-2678 ◽  
Author(s):  
Yan Yun Zhu ◽  
Xiao Li Zhu ◽  
Fang She Yang
Keyword(s):  
Rhizosphere Soil ◽  
Barrier Discharge ◽  
Nitrogenase Activity ◽  
Nitrogen Fixing ◽  
Bacterial Strains ◽  
Dbd Plasma ◽  
Nitrogen Fixing Bacteria ◽  
Free Living ◽  
Reduction Assay ◽  
Rrna Sequencing

Nitrogen-fixing bacteria were screened from the rhizosphere soil of plants in Shaanxi in China. 36 free-living nitrogen-fixing bacterial strains were isolated and their nitrogenase activity were determined by acetylene reduction assay (ARA), two strains named FLNB03 and FLNB09 with higher nitrogenase activity were isolated and identified by 16S rRNA sequencing. The datum showed that FLNB03 was similar to Acinetobacter and their similarity reached 99%, FLNB09 was similar to Agrobacterium sp. and their similarity reached 99%. Then both of them were treated using Dielectric Barrier Discharge (DBD) plasma for mutation and their mutants called FLNB03-2 and FLNB09-3 were obtained. The nitrogenase activity of FLNB03-2 was 0.61±0.10 nmol•107cfu-1•h-1, and that of FLNB09-3 was 0.40±0.05 nmol•107cfu-1•h-1, their nitrogenase activity increased by 22.00% and 14.29% than their original bacteria respectively. FLNB03-2 and FLNB09-3 might be used as microbial fertilizer.

Sign in / Sign up

Export Citation Format

Share Document