Regulation of Tissue-Specific Alternative Splicing: C. elegans as a Model System

2016 ◽  
pp. 229-261 ◽  
Author(s):  
Xicotencatl Gracida ◽  
Adam D. Norris ◽  
John A. Calarco
Keyword(s):  
Alternative Splicing ◽  
Model System ◽  
Tissue Specific ◽  
C Elegans ◽  
Specific Alternative

scholarly journals Tissue-specific isoforms of the single C. elegans Ryanodine receptor gene unc-68 control specific functions

PLoS Genetics ◽  
2020 ◽  
Vol 16 (10) ◽  
pp. e1009102
Author(s):  
Filipe Marques ◽  
Saurabh Thapliyal ◽  
Avelino Javer ◽  
Priyanka Shrestha ◽  
André E. X. Brown ◽  
...  
Keyword(s):  
Alternative Splicing ◽  
Receptor Gene ◽  
Ryanodine Receptors ◽  
Computer Assisted ◽  
Alternative Promoters ◽  
Tissue Specific ◽  
C Elegans ◽  
Specific Alternative ◽  
And Function ◽  
Different Tissues

Ryanodine receptors (RyR) are essential regulators of cellular calcium homeostasis and signaling. Vertebrate genomes contain multiple RyR gene isoforms, expressed in different tissues and executing different functions. In contrast, invertebrate genomes contain a single RyR-encoding gene and it has long been proposed that different transcripts generated by alternative splicing may diversify their functions. Here, we analyze the expression and function of alternative exons in the C. elegans RyR gene unc-68. We show that specific isoform subsets are created via alternative promoters and via alternative splicing in unc-68 Divergent Region 2 (DR2), which actually corresponds to a region of high sequence variability across vertebrate isoforms. The expression of specific unc-68 alternative exons is enriched in different tissues, such as in body wall muscle, neurons and pharyngeal muscle. In order to infer the function of specific alternative promoters and alternative exons of unc-68, we selectively deleted them by CRISPR/Cas9 genome editing. We evaluated pharyngeal function, as well as locomotor function in swimming and crawling with high-content computer-assisted postural and behavioral analysis. Our data provide a comprehensive map of the pleiotropic impact of isoform-specific mutations and highlight that tissue-specific unc-68 isoforms fulfill distinct functions. As a whole, our work clarifies how the C. elegans single RyR gene unc-68 can fulfill multiple tasks through tissue-specific isoforms, and provide a solid foundation to further develop C. elegans as a model to study RyR channel functions and malfunctions.

Tissue-specific alternative splicing of BK channel transcripts in Drosophila

2006 ◽  
Vol 5 (4) ◽  
pp. 329-339 ◽  
Author(s):  
J. Y. Yu ◽  
A. B. Upadhyaya ◽  
N. S. Atkinson
Keyword(s):  
Alternative Splicing ◽  
Bk Channel ◽  
Tissue Specific ◽  
Specific Alternative

Tissue-specific alternative splicing of neurofibromatosis 1 (NF1) mRNA

1994 ◽  
Vol 48 (8-9) ◽  
pp. 365-372 ◽  
Author(s):  
G. Danglot ◽  
C. Teinturier ◽  
A. Duverger ◽  
A. Bernheim
Keyword(s):  
Alternative Splicing ◽  
Neurofibromatosis 1 ◽  
Tissue Specific ◽  
Specific Alternative

scholarly journals TISA: Tissue-specific Alternative Splicing in Human and Mouse Genes

DNA Research ◽  
2006 ◽  
Vol 13 (5) ◽  
pp. 229-243 ◽  
Author(s):  
Seung-Jae Noh ◽  
Kyooyeol Lee ◽  
Hyojung Paik ◽  
Cheol-Goo Hur
Keyword(s):  
Alternative Splicing ◽  
Tissue Specific ◽  
Specific Alternative ◽  
Human And Mouse
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