Automated Software Composition—A Top View

Author(s):  
Felix Mohr
2013 ◽  
Vol 39 (1) ◽  
pp. 63-79 ◽  
Author(s):  
Sven Apel ◽  
Christian Kastner ◽  
Christian Lengauer

Author(s):  
J. Frank ◽  
P.-Y. Sizaret ◽  
A. Verschoor ◽  
J. Lamy

The accuracy with which the attachment site of immunolabels bound to macromolecules may be localized in electron microscopic images can be considerably improved by using single particle averaging. The example studied in this work showed that the accuracy may be better than the resolution limit imposed by negative staining (∽2nm).The structure used for this demonstration was a halfmolecule of Limulus polyphemus (LP) hemocyanin, consisting of 24 subunits grouped into four hexamers. The top view of this structure was previously studied by image averaging and correspondence analysis. It was found to vary according to the flip or flop position of the molecule, and to the stain imbalance between diagonally opposed hexamers (“rocking effect”). These findings have recently been incorporated into a model of the full 8 × 6 molecule.LP hemocyanin contains eight different polypeptides, and antibodies specific for one, LP II, were used. Uranyl acetate was used as stain. A total of 58 molecule images (29 unlabelled, 29 labelled with antl-LPII Fab) showing the top view were digitized in the microdensitometer with a sampling distance of 50μ corresponding to 6.25nm.


Author(s):  
Nicolas Boisset ◽  
Jean-Christophe Taveau ◽  
Jean Lamy ◽  
Terence Wagenknecht ◽  
Michael Radermacher ◽  
...  

Hemocyanin, the respiratory pigment of the scorpion Androctonus australis is composed of 24 kidney shaped subunits. A model of architecture supported by many indirect arguments has been deduced from electron microscopy (EM) and immuno-EM. To ascertain, the disposition of the subunits within the oligomer, the 24mer was submitted to three-dimensional reconstruction by the method of single-exposure random-conical tilt series.A sample of native hemocyanin, prepared with the double layer negative staining technique, was observed by transmisson electron microscopy under low-dose conditions. Six 3D-reconstructions were carried out indenpendently from top, side and 45°views. The results are composed of solid-body surface representations, and slices extracted from the reconstruction volume.The main two characters of the molecule previously reported by Van Heel and Frank, were constantly found in the solid-body surface representations. These features are the presence of two different faces called flip and flop and a rocking of the molecule around an axis passing through diagonnally opposed hexamers. Furthermore, in the solid-body surface of the top view reconstruction, the positions and orientations of the bridges connecting the half molecules were found in excellent agreement with those predicted by the model.


2017 ◽  
Author(s):  
Fawaz Alqahtani ◽  
Fabrizio Messina ◽  
Elzene Kruger ◽  
Heerunpal Gill ◽  
Michael Ellis ◽  
...  

Author(s):  
Priyanka R. Patil ◽  
Shital A. Patil

Similarity View is an application for visually comparing and exploring multiple models of text and collection of document. Friendbook finds ways of life of clients from client driven sensor information, measures the closeness of ways of life amongst clients, and prescribes companions to clients if their ways of life have high likeness. Roused by demonstrate a clients day by day life as life records, from their ways of life are separated by utilizing the Latent Dirichlet Allocation Algorithm. Manual techniques can't be utilized for checking research papers, as the doled out commentator may have lacking learning in the exploration disciplines. For different subjective views, causing possible misinterpretations. An urgent need for an effective and feasible approach to check the submitted research papers with support of automated software. A method like text mining method come to solve the problem of automatically checking the research papers semantically. The proposed method to finding the proper similarity of text from the collection of documents by using Latent Dirichlet Allocation (LDA) algorithm and Latent Semantic Analysis (LSA) with synonym algorithm which is used to find synonyms of text index wise by using the English wordnet dictionary, another algorithm is LSA without synonym used to find the similarity of text based on index. LSA with synonym rate of accuracy is greater when the synonym are consider for matching.


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