Biogeochemical Technology for Monitoring of Cleaning of Soil Polluted by Gas Condensate and Neutralization of Its Sludge by Means of Enzyme Activity Analysis

2016 ◽  
pp. 193-200 ◽  
Author(s):  
Rauf V. Galiulin ◽  
Vladimir N. Bashkin ◽  
Rosa A. Galiulina
Keyword(s):  
Enzyme Activity ◽  
Activity Analysis ◽  
Gas Condensate

scholarly journals Purification and subunit structure of argininosuccinate lyase from Chlamydomonas reinhardi

1977 ◽  
Vol 167 (1) ◽  
pp. 71-75 ◽  
Author(s):  
R F Matagne ◽  
J P Schlösser
Keyword(s):  
Enzyme Activity ◽  
Crude Extract ◽  
Enzyme Preparation ◽  
Sodium Dodecyl ◽  
Gel Filtration ◽  
Deae Cellulose ◽  
Activity Analysis ◽  
Disc Electrophoresis ◽  
Subunit Structure ◽  
Argininosuccinate Lyase

Argininosuccinate lyase (EC 4.3.2.1) was purified by (NH4)2SO4 fractionation, chromatography on DEAE-cellulose and gel filtration on Sephadex G-200. The final enzyme preparation was purified 46-fold compared with the crude extract. Electrophoresis of this preparation revealed three bands, the major one having the enzyme activity. Analysis of the enzyme by gel filtration and by disc electrophoresis (in two different concentrations of acrylamide) gave mol.wts. of 200000 (+/- 15000) and 190000 (+/- 20000) respectively. Treatment with sodium dodecyl sulphate and mercaptoethanol dissociated the enzyme into subunits of mol.wt. 39000 (+/-2000). The results are indicative of the multimeric structure of the enzyme, which is composed of five (perhaps four or six) identical subunits.

scholarly journals Polyhydroxyalkanoates (PHA) production from synthetic waste using Pseudomonas pseudoflava : PHA synthase enzyme activity analysis from P. pseudoflava and P. palleronii

2017 ◽  
Vol 234 ◽  
pp. 99-105 ◽  
Author(s):  
M. Venkateswar Reddy ◽  
Yasuteru Mawatari ◽  
Rui Onodera ◽  
Yuki Nakamura ◽  
Yuka Yajima ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Activity Analysis ◽  
Pha Synthase ◽  
Pha Production

GAA gene mutation detection following clinical evaluation and enzyme activity analysis in Azeri Turkish patients with Pompe disease

2020 ◽  
Vol 35 (7) ◽  
pp. 1127-1134
Author(s):  
Jalal Gharesouran ◽  
Abbas Jalaiei ◽  
Aida Hosseinzadeh ◽  
Soudeh Ghafouri-Fard ◽  
Zeinab Mokhtari ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Gene Mutation ◽  
Clinical Evaluation ◽  
Pompe Disease ◽  
Mutation Detection ◽  
Activity Analysis ◽  
Turkish Patients ◽  
Gaa Gene
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