A Stochastic Single Cell Based Model of BrdU Measured Hematopoietic Stem Cell Kinetics

2008 ◽  
pp. 387-401 ◽  
Author(s):  
Richard C. van der Wath ◽  
Pietro Lio’
Keyword(s):  
Stem Cell ◽  
Single Cell ◽  
Hematopoietic Stem Cell ◽  
Cell Kinetics ◽  
Hematopoietic Stem

scholarly journals Cellular Barcoding Links B-1a B Cell Potential to a Fetal Hematopoietic Stem Cell State at the Single-Cell Level

Immunity ◽  
2016 ◽  
Vol 45 (2) ◽  
pp. 346-357 ◽  
Author(s):  
Trine A. Kristiansen ◽  
Elin Jaensson Gyllenbäck ◽  
Alya Zriwil ◽  
Tomas Björklund ◽  
Jeremy A. Daniel ◽  
...  
Keyword(s):  
Stem Cell ◽  
B Cell ◽  
Single Cell ◽  
Hematopoietic Stem Cell ◽  
Single Cell Level ◽  
Cell Level ◽  
Cell Potential ◽  
Hematopoietic Stem ◽  
Stem Cell State ◽  
Cellular Barcoding

scholarly journals High-Resolution Single-Cell DNA Methylation Measurements Reveal Epigenetically Distinct Hematopoietic Stem Cell Subpopulations

2018 ◽  
Vol 11 (2) ◽  
pp. 578-592 ◽  
Author(s):  
Tony Hui ◽  
Qi Cao ◽  
Joanna Wegrzyn-Woltosz ◽  
Kieran O'Neill ◽  
Colin A. Hammond ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Stem Cell ◽  
High Resolution ◽  
Single Cell ◽  
Hematopoietic Stem Cell ◽  
Hematopoietic Stem ◽  
Cell Subpopulations ◽  
Resolution Single

scholarly journals Single Cell Analysis of Circulating Endothelial Cells in Allogeneic Hematopoietic Stem Cell Transplant; To Whom Do They Belong: Host or Donor?

Blood ◽  
2019 ◽  
Vol 134 (Supplement_1) ◽  
pp. 4885-4885
Author(s):  
Camillo Almici ◽  
Francesca Fontana ◽  
Giovanna Piovani ◽  
Piera Balzarini ◽  
Arabella Neva ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Stem Cell ◽  
Single Cell ◽  
Hematopoietic Stem Cell ◽  
Risk Score ◽  
Hematopoietic Stem Cell Transplant ◽  
Stem Cell Transplant ◽  
Cell Transplant ◽  
Hematopoietic Stem ◽  
Str Profile

Background. We recently reported that Circulating Endothelial Cell (CEC) count changes represent a promising marker to monitor endothelial damage in patients undergoing allogeneic hematopoietic stem cell transplant (allo-HSCT), potentially becoming a valuable tool in the diagnostic definition of GVHD. Besides confirming an increase of CEC counts at GVHD onset, we repeatedly documented at time of engraftment statistically significant higher numbers of CEC in patients who will not manifest GVHD in comparison to patients in which GVHD will be diagnosed (Transplantation 2014,98:706-12; Bone Marrow Transplantation 2017,52:1637-42; Scientific Reports 2019,9:1-12). Recent knowledges in organ transplant pointed out that endothelial cells from the grafted organ, besides being a continuous source of alloantigens, can downregulate alloreactivity exerting tolerogenic responses. By inference to the allo-HSCT field, it could be envisaged that presence of donor CEC could induce protective effects on alloreactivity. Methods. We planned a study to test the hypothesis that at time of engraftment, CEC present in peripheral blood (PB), besides coming from cells shedding from patient vasculature, could partly belong to donor, originating from the cellular graft. Therefore, in an exploratory set, we performed FISH analysis on flowcytometry-sorted CEC (CD45neg/CD34bright/CD146pos, Lyotube #623920, BD Biosciences) (n=3) and on whole PB derived culture-expanded CEC (n=3) (EGM-2 BulletKit, Lonza), obtained at engraftment in sex-mismatched allo-HSCT. In the confirmatory set (n=15), single CEC were recovered from PB, at engraftment (T1) and at 90 days (T2) after allo-HSCT, through the DEPArrayTM technology (Menarini Silicon Biosystems), after preliminary bulk separation step carried out with the CellSearch® System. Single recovered CEC was whole genome amplified (Ampli1™ WGA Kit) and short tandem repeat (STR) profile determined (Ampli 1TM STR kit) on each single CEC. To confirm host/donor origin, single CEC STR profile was compared to that determined on patient and donor cells before allo-HSCT. Moreover, donor CEC presence was evaluated by CISH analysis on formaline fixed and paraffin-embedded biopsy sections obtained at least three months after sex mismatched allo-HSCT. Results. By positive findings of the exploratory set, we proved, at the single cell level in the confirmatory set, the presence of donor CEC at engraftment (T1) in 4 out of 15 patients (Table 1). Of them, 2 did not manifested GVHD, despite a GVHD risk score of 2, and the other 2 presented GVHD grade I. On the contrary, among the 10 patients in whom no donor CEC were detected, 6 experienced GVHD grade II-III, while 4 did not manifested GVHD, despite a 1-3 GVHD risk score. Conclusions. Our data represent the proof of principle that donor CEC may flow in host PB early on from hematopoietic recovery and seldom persist thereafter at steady-state conditions, being potentially embedded in host vascular wall. These puzzling findings suggest that neovascularization takes place in parallel with hematopoietic engraftment and could provide further clues on shedding light on tissue tolerance in the context of GVHD, opening up paradoxical scenarios on the protective role potentially played by donor CEC. Disclosures Fontana: Menarini Silicon Biosystem: Employment. Rotta:BD Biosciences Italia: Employment. Manaresi:Menarini SIlicon Biosystem: Employment, Membership on an entity's Board of Directors or advisory committees.

scholarly journals Single cell transplantation—Golden standard of hematopoietic stem cell heterogeneity study

2017 ◽  
Vol 47 (12) ◽  
pp. 1353-1362
Author(s):  
Hideo EMA ◽  
Sha HAO ◽  
Fang DONG ◽  
Tao CHENG ◽  
XiaoFang WANG
Keyword(s):  
Stem Cell ◽  
Single Cell ◽  
Cell Transplantation ◽  
Hematopoietic Stem Cell ◽  
Cell Heterogeneity ◽  
Hematopoietic Stem ◽  
Golden Standard

Leukocyte telomere dynamics and human hematopoietic stem cell kinetics during somatic growth

2009 ◽  
Vol 37 (4) ◽  
pp. 514-524 ◽  
Author(s):  
Igor Sidorov ◽  
Masayuki Kimura ◽  
Anatoli Yashin ◽  
Abraham Aviv
Keyword(s):  
Stem Cell ◽  
Hematopoietic Stem Cell ◽  
Cell Kinetics ◽  
Somatic Growth ◽  
Hematopoietic Stem ◽  
Telomere Dynamics

X-linked genetic factors regulate hematopoietic stem-cell kinetics in females

Blood ◽  
2000 ◽  
Vol 95 (7) ◽  
pp. 2449-2451 ◽  
Author(s):  
Kaare Christensen ◽  
Marianne Kristiansen ◽  
Heidi Hagen-Larsen ◽  
Axel Skytthe ◽  
Lise Bathum ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hematopoietic Stem Cell ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Genetic Factors ◽  
Cell Kinetics ◽  
Elderly Women ◽  
Cell Populations ◽  
Peripheral Blood Cells ◽  
Hematopoietic Stem

Abstract X inactivation makes females mosaics for 2 cell populations, usually with an approximate 1:1 distribution. Skewing of this distribution in peripheral blood cells is more common among elderly women.1–3 The depletion of hematopoietic stem cells followed by random differentiation may explain the acquired skewing with age.4 However, an animal model suggests that selection processes based on X-linked genetic factors are involved.5 We studied peripheral blood cells from 71 monozygotic twin pairs aged 73 to 93 years and from 33 centenarians, and we found that with age, 1 of the cell populations becomes predominant for most women. We also observed a strong tendency for the same cell line to become predominant in 2 co-twins. This suggests that X-linked genetic factors influence human hematopoietic stem cell kinetics. The fact that females have 2 cell lines with different potentials could be one of the reasons women live longer than men.

scholarly journals An X chromosome gene regulates hematopoietic stem cell kinetics

1998 ◽  
Vol 95 (7) ◽  
pp. 3862-3866 ◽  
Author(s):  
J. L. Abkowitz ◽  
M. Taboada ◽  
G. H. Shelton ◽  
S. N. Catlin ◽  
P. Guttorp ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hematopoietic Stem Cell ◽  
X Chromosome ◽  
Cell Kinetics ◽  
Hematopoietic Stem ◽  
Chromosome Gene

scholarly journals Resolving Fates and Single-Cell Transcriptomes of Hematopoietic Stem Cell Clones by PolyloxExpress Barcoding

2020 ◽  
Vol 27 (3) ◽  
pp. 383-395.e8 ◽  
Author(s):  
Weike Pei ◽  
Fuwei Shang ◽  
Xi Wang ◽  
Ann-Kathrin Fanti ◽  
Alessandro Greco ◽  
...  
Keyword(s):  
Stem Cell ◽  
Single Cell ◽  
Hematopoietic Stem Cell ◽  
Hematopoietic Stem ◽  
Cell Clones
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