A Hybrid Approach to Estimate True Density Function for Gene Expression Data

2011 ◽  
pp. 44-54
Author(s):  
Ganesh Kumar Pugalendhi ◽  
Mahibha David ◽  
Aruldoss Albert Victoire
Keyword(s):  
Gene Expression ◽  
Density Function ◽  
Gene Expression Data ◽  
Hybrid Approach ◽  
Expression Data ◽  
True Density

scholarly journals The Role of Scale in the Estimation of Cell-type Proportions

2019 ◽  
Author(s):  
Gregory J. Hunt ◽  
Johann A. Gagnon-Bartsch
Keyword(s):  
Gene Expression ◽  
Gene Expression Data ◽  
Hybrid Approach ◽  
Expression Data ◽  
Cell Type ◽  
Statistical Efficiency ◽  
Gene Expressions ◽  
New Approach ◽  
Cell Type Composition ◽  
Type Composition

ABSTRACTComplex tissues are composed of a large number of different types of cells, each involved in a multitude of biological processes. Consequently, an important component to understanding such processes is understanding the cell-type composition of the tissues. Estimating cell type composition using high-throughput gene expression data is known as cell-type deconvolution. In this paper, we first summarize the extensive deconvolution literature by identifying a common regression-like approach to deconvolution. We call this approach the Unified Deconvolution-as-Regression (UDAR) framework. While methods that fall under this framework all use a similar model, they fit using data on different scales. Two popular scales for gene expression data are logarithmic and linear. Unfortunately, each of these scales has problems in the UDAR framework. Using log-scale gene expressions proposes a biologically implausible model and using linear-scale gene expressions will lead to statistically inefficient estimators. To overcome these problems, we propose a new approach for cell-type deconvolution that works on a hybrid of the two scales. This new approach is biologically plausible and improves statistical efficiency. We compare the hybrid approach to other methods on simulations as well as a collection of eleven real benchmark datasets. Here, we find the hybrid approach to be accurate and robust.deconvolution, gene expression, microarray, RNA-seq

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