Continuous Recording of K+ and Ca2+ Pancreatic Juice of Conscious Rats Provided with an Extracorporeal Pancreatic Loop

The Effect of Glucagon on the Formation of Pancreatic Juice and Bile in the Rat

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y73-001 ◽

1973 ◽

Vol 51 (1) ◽

pp. 1-5 ◽

Cited By ~ 20

Author(s):

H. M. Shaw ◽

T. J. Heath

Keyword(s):

Pancreatic Juice ◽

Pancreatic Glucagon ◽

Conscious Rats ◽

Intravenous Infusions

The effect of porcine pancreatic glucagon on the formation of pancreatic juice and bile was studied in conscious rats. When the rats were fasting, intravenous infusions of glucagon were associated with depressions in the flow, and in the output of bicarbonate and protein in pancreatic juice (p < 0.001). By contrast, infusions of glucagon to rats during feeding were associated with increases in the formation of pancreatic juice, particularly the volume and bicarbonate fraction. The responses were greater than those recorded in rats that did not receive exogenous glucagon during feeding (p < 0.01). Glucagon did not appear to influence the formation of bile.

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Interactions between Bile and Pancreatic Juice Diversions on Cholecystokinin Release and Pancreas in Conscious Rats

Experimental Biology and Medicine ◽

10.3181/00379727-192-42976 ◽

1989 ◽

Vol 192 (2) ◽

pp. 182-186 ◽

Cited By ~ 29

Author(s):

R. Nakamura ◽

K. Miyasaka ◽

A. Funakoshi ◽

K. Kitani

Keyword(s):

Pancreatic Juice ◽

Conscious Rats

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Atropine-resistant secretion of a putative luminal CCK-releasing peptide in conscious rats

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.1999.276.1.g287 ◽

1999 ◽

Vol 276 (1) ◽

pp. G287-G292 ◽

Cited By ~ 1

Author(s):

Kyoko Miyasaka ◽

Kayoko Tateishi ◽

Masao Masuda ◽

Atsuo Jimi ◽

Akihiro Funakoshi

Keyword(s):

Small Intestine ◽

Pancreatic Juice ◽

Intestinal Lumen ◽

Trypsin Activity ◽

Luminal Content ◽

Conscious Rats ◽

Cholinergic Regulation ◽

Before And After ◽

Small Intestinal ◽

Cck Release

The changes in levels of the newly discovered luminal CCK-releasing factor (LCRF) in the small intestinal lumen before and after bile-pancreatic juice diversion in conscious rats were examined by a specific RIA. Moreover, we also examined whether LCRF secretion was under cholinergic control. Anti-LCRF antiserum was raised in rabbits, and a sensitive RIA was established. The localization of LCRF was examined by immunohistochemistry. The luminal content of LCRF was significantly increased by bile-pancreatic juice diversion, during which luminal trypsin activity was eliminated. The increase in luminal LCRF content was not inhibited by intravenous infusion of atropine. The changes in plasma levels of CCK and pancreatic secretion were similar to those in luminal LCRF contents. LCRF immunostaining was observed in villus tip enterocytes of the small intestine and was most prominent in the duodenal portion. These results support our original hypothesis that LCRF may be released spontaneously into the small intestinal lumen from the villus tip enterocytes and its intraluminal degradation by proteases regulates CCK release. Furthermore, LCRF release was not subject to cholinergic regulation.

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