Ro 10-9359 Retinoid Inhibits Both in vitro Epidermal Cell Proliferation and Differentiation

Abstract Background: Skeletal muscle injury leads to loss of muscle function that lasts well into recovery and can be permanent. Application of the novel bio-scaffold termed porcine-derived urinary bladder matrix (UBM) has a potential benefit to mitigate injury through tissue regeneration. To date, findings of potential benefit in animal models were limited to short assessment times. The purpose of this study was to investigate whether UBM treatment 14 days after injury sustainably improves the recovery of muscle function in injured mice. Methods: C57BL/6 adult male mice received bilateral laceration injuries on the gastrocnemius (GN) muscle under anesthesia and were then treated with vehicle or 150 µg of UBM nanoparticles. Treatment was applied immediately after injury or 14 days later. Muscle isometric force was measured 60 days after injury. Previous time course analyses have shown that muscle function did not start to improve until after 42 days after injury. Therefore, we designed a second experiment to trace the time course of UBM effects on muscle function recovery by measuring the isometric muscle force at 49 and 90 days after injury. In vitro, we analyzed the effects of UBM on muscle cell proliferation and differentiation. Results: UBM promotes muscle cell proliferation and differentiation. Twitch (Pt), tetanic (Po) force and maximal fatigue were significantly decreased in the injured mice on day 60. Muscle fatigue maximum force significantly recovered when UBM treatment was applied 14 days after injury (p<0.05) but not when UBM was applied immediately after the injury. Time course analysis demonstrated that UBM improvement of Pt and Po was evident by day 49 after injury (p<0.05). However, no further muscle function improvement was observed on day 90. Conclusions: Delayed treatment with the UBM improves muscle function recovery following laceration injury starting 49 days after injury. These effects may be mediated by improvements in muscle cell proliferation and differentiation. This animal model is suitable to test other therapeutic strategies to improve muscle function after injury.

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The Effects of Laser Irradiation on Osteoblast and Osteosarcoma Cell Proliferation and Differentiation in Vitro

Photomedicine and Laser Surgery ◽

10.1089/pho.2007.2055 ◽

2007 ◽

Vol 25 (4) ◽

pp. 275-280 ◽

Cited By ~ 113

Author(s):

A.C.M. Renno ◽

P.A. McDonnell ◽

N.A. Parizotto ◽

E.-L. Laakso

Keyword(s):

Cell Proliferation ◽

Laser Irradiation ◽

Osteosarcoma Cell ◽

Cell Proliferation And Differentiation ◽

Proliferation And Differentiation

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Monitoring of stem cell proliferation and differentiation using a permittivity-responsive biointerface

MRS Proceedings ◽

10.1557/proc-773-n7.12 ◽

2003 ◽

Vol 773 ◽

Cited By ~ 1

Author(s):

P.O. Bagnaninchi ◽

M. Dikeakos ◽

T. Veres ◽

M. Tabrizian

Keyword(s):

Cell Proliferation ◽

Main Approach ◽

Stem Cell Proliferation ◽

Cell Proliferation And Differentiation ◽

Proliferation And Differentiation ◽

Network Analyser ◽

Culture Growth ◽

Microporous Scaffolds ◽

Cells Cultured

AbstractThe main approach of in-vitro bone engineering is based on the use of stem cells cultured in microporous scaffolds. In order to quickly and non-destructively assess the different steps of bone formation, we have proposed the use of a permittivity-responsive sensor to monitor the microporous scaffold, which in this instance acts as a biointerface. The aim of this study is to monitor and characterize the growth and differentiation of cells in the microporous biointerface through CP measurements. Measurements are performed throughout the entire process of the cells' culture, growth and differentiation using a dielectric probe and a vector network analyser under sterile conditions. This concept of a permittivity-responsive biointerface will lead to sensitive biosensors especially adapted for use in tissue engineering.

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The role of IGF1 on the differentiation of prolactin secreting cells in the mouse anterior pituitary

Journal of Endocrinology ◽

10.1677/joe-09-0232 ◽

2009 ◽

Vol 203 (2) ◽

pp. 231-240 ◽

Cited By ~ 13

Author(s):

Tamiki Hikake ◽

Shinji Hayashi ◽

Taisen Iguchi ◽

Tomomi Sato

Keyword(s):

Cell Proliferation ◽

Progenitor Cells ◽

Anterior Pituitary ◽

Cell Number ◽

Postnatal Period ◽

Gh Cells ◽

Cell Proliferation And Differentiation ◽

Proliferation And Differentiation

IGF1 knockout (IGF1KO) mice show a reduced number of prolactin (PRL) producing cells (PRL cells); however, the role of IGF1 in PRL cell proliferation and differentiation in immature mice is unclear. In this study, ontogenic changes in the percentages of PRL cells, GH producing cells (GH cells), and 5-bromo-2′-deoxyuridine (BrdU)-labeled cells in the anterior pituitary of male IGF1KO mice during the postnatal period were investigated. The percentage of PRL cells in IGF1KO mice was significantly lower at day 20 compared with that in wild-type (WT) mice, while GH cells in IGF1KO mice were significantly increased from day 10. From days 5 to 20, the percentage of BrdU-labeled cells in WT and IGF1KO mice was similar. PRL cells and GH cells are thought to originate from the same progenitor cells, therefore, PRL cells in IGF1KO mice are not able to differentiate because progenitor cells have already committed to be GH cells. However, IGF1, 17β-estradiol (E2), epidermal growth factor (EGF), or IGF1 plus E2 treatments increased the PRL cell number in the pituitaries in vitro of 10-day-old WT and IGF1KO mice. This fact suggests that these factors are involved in PRL cell proliferation and differentiation. In addition, the increase of PRL cells in IGF1KO mice stimulated by E2 or EGF was less than that of WT mice. Thus, IGF1 plays a crucial role in PRL cell proliferation and differentiation in mouse pituitaries by regulating the differentiation of progenitor cells and mediating the actions of E2 and EGF.

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