Phosphoinositides in Cell Suspension Cultures of Glycine Max

1987 ◽  
pp. 151-155
Author(s):  
Christina Hoffmann ◽  
Hans Grisebach
Keyword(s):  
Glycine Max ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures

Über den Abbau von Flavonolen in pflanzlichen Zellsuspensionskulturen / Degradation of Flavonols in Plant Suspension Cultures

1972 ◽  
Vol 27 (8) ◽  
pp. 946-954 ◽  
Author(s):  
Wolfgang Hösel ◽  
Paul D. Shaw ◽  
Wolfgang Barz
Keyword(s):  
Salicylic Acid ◽  
Glycine Max ◽  
Cell Suspension ◽  
Cicer Arietinum ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Enzyme Preparations ◽  
Cicer Arietinum L

The flavonols kaempferol, quercetin and isorhamnetin were labelled with 14C by keeping seven day old Cicer arietinum L. plants in an atmosphere of 14CO2 for five days. The purified (U-14C) flavonols were applied to cell suspension cultures of Cicer arietinum L., Phaseolus aureus Roxb., Glycine max and Petroselinum hortense. Based on the rates of 14CO2 formation and distribution of radioactivity after fractionation of the cells, the flavonols were shown to be catabolized to a very high extent.All four cell suspension cultures possess the enzymatic activity transforming flavonols to the recently discovered 2,3-dihydroxyflavanones. Upon incubation of the flavonols datiscetin and kaempferol with enzyme preparations from Cicer arietinum L. cell suspension cultures, it was demonstrated that the enzymatically formed 2,3-dihydroxyflavanones are further transformed in an enzyme catalyzed reaction. Salicylic acid was found as a degradation fragment of ring B of the 2,3,5,7,2′-pentahydroxyflavanone derived from datiscetin. Neither phloroglucinol nor phloroglucinol carboxylic acid were observed as metabolites of ring A. These in vitro findings were further substantiated by in vivo data because the flavonols kaempferol, quercetin and datiscetin when applied to cell suspension cultures of Cicer arietinum L. and Glycine max gave rise to para-hydroxybenzoic acid, protocatechuic acid and salicylic acid, respectively. It was thus concluded that flavonols are catabolized via 2,3-dihydroxyflavanones with the B-ring liberated as the respective benzoic acid. The data are discussed in connection with earlier findings on the catabolism of chalcones, cinnamic and benzoic acids.

VARIATIONS IN CHROMOSOME NUMBER AND STRUCTURE IN PLANT CELLS GROWN IN SUSPENSION CULTURES

1970 ◽  
Vol 12 (2) ◽  
pp. 297-301 ◽  
Author(s):  
K. N. Kao ◽  
R. A. Miller ◽  
O. L. Gamborg ◽  
B. L. Harvey
Keyword(s):  
Glycine Max ◽  
Chromosome Number ◽  
Cell Suspension ◽  
Plant Cells ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Triticum Monococcum ◽  
Melilotus Alba ◽  
Haplopappus Gracilis ◽  
Chromosomal Changes

Cell suspension cultures of Triticum monococcum, Triticiam aestivum, Glycine max, Melilotus alba, and Haplopappus gracilis, were examined to determine whether chromosomal changes had occurred during culture. All cultures except H. gracilis showed change in chromosome number and the two species of Triticum showed abnormal karyotypes.

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