Bioreactors for the Large-Scale Culture of Plant Cells

1988 ◽  
pp. 104-122 ◽  
Author(s):  
T. Hashimoto ◽  
S. Azechi
Keyword(s):  
Large Scale ◽  
Plant Cells ◽  
Large Scale Culture

Large-Scale Culture of Plant Cells

2003 ◽  
pp. 477-494
Author(s):  
Alan H. Scragg ◽  
Michael W. Fowler
Keyword(s):  
Large Scale ◽  
Plant Cells ◽  
Large Scale Culture

scholarly journals An efficient direct screening system for microorganisms that activate plant immune responses based on plant–microbe interactions using cultured plant cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mari Kurokawa ◽  
Masataka Nakano ◽  
Nobutaka Kitahata ◽  
Kazuyuki Kuchitsu ◽  
Toshiki Furuya
Keyword(s):  
Immune Responses ◽  
Plant Defense ◽  
Pseudomonas Syringae ◽  
Large Scale ◽  
Plant Cells ◽  
Defense Responses ◽  
Root Tip ◽  
General Strategy ◽  
Plant Defense Responses ◽  
Microbe Interactions

AbstractMicroorganisms that activate plant immune responses have attracted considerable attention as potential biocontrol agents in agriculture because they could reduce agrochemical use. However, conventional methods to screen for such microorganisms using whole plants and pathogens are generally laborious and time consuming. Here, we describe a general strategy using cultured plant cells to identify microorganisms that activate plant defense responses based on plant–microbe interactions. Microbial cells were incubated with tobacco BY-2 cells, followed by treatment with cryptogein, a proteinaceous elicitor of tobacco immune responses secreted by an oomycete. Cryptogein-induced production of reactive oxygen species (ROS) in BY-2 cells served as a marker to evaluate the potential of microorganisms to activate plant defense responses. Twenty-nine bacterial strains isolated from the interior of Brassica rapa var. perviridis plants were screened, and 8 strains that enhanced cryptogein-induced ROS production in BY-2 cells were selected. Following application of these strains to the root tip of Arabidopsis seedlings, two strains, Delftia sp. BR1R-2 and Arthrobacter sp. BR2S-6, were found to induce whole-plant resistance to bacterial pathogens (Pseudomonas syringae pv. tomato DC3000 and Pectobacterium carotovora subsp. carotovora NBRC 14082). Pathogen-induced expression of plant defense-related genes (PR-1, PR-5, and PDF1.2) was enhanced by the pretreatment with strain BR1R-2. This cell–cell interaction-based platform is readily applicable to large-scale screening for microorganisms that enhance plant defense responses under various environmental conditions.

Microfluidic PDMS (Polydimethylsiloxane) Bioreactor for Large-Scale Culture of Hepatocytes

2004 ◽  
Vol 20 (3) ◽  
pp. 750-755 ◽  
Author(s):  
E. Leclerc ◽  
Y. Sakai ◽  
T. Fujii
Keyword(s):  
Large Scale ◽  
Large Scale Culture ◽  
Culture Of Hepatocytes

Large Scale Culture of Ginseng Adventitious Roots for Production of Ginsenosides

2009 ◽  
pp. 151-176 ◽  
Author(s):  
Kee-Yoeup Paek ◽  
Hosakatte Niranjana Murthy ◽  
Eun-Joo Hahn ◽  
Jian-Jiang Zhong
Keyword(s):  
Adventitious Roots ◽  
Large Scale ◽  
Large Scale Culture

Large-Scale Culture of Vero Cells on GT-2 Microcarrier in Cell Culture Bioreactors

1992 ◽  
pp. 347-349
Author(s):  
Chen Yinliang ◽  
Dong Shupei ◽  
Gu Xiaohua ◽  
Yan Chun ◽  
Song Jiali ◽  
...  
Keyword(s):  
Cell Culture ◽  
Large Scale ◽  
Vero Cells ◽  
Large Scale Culture

Large Scale Culture of Pluripotent Stem Cells for Therapy

Stem Cells ◽  
2010 ◽  
pp. 127-154
Author(s):  
Mahendra Rao ◽  
Mohan C. Vemuri
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Large Scale ◽  
Large Scale Culture
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