Large Scale Culture of Ginseng Adventitious Roots for Production of Ginsenosides

2009 ◽  
pp. 151-176 ◽  
Author(s):  
Kee-Yoeup Paek ◽  
Hosakatte Niranjana Murthy ◽  
Eun-Joo Hahn ◽  
Jian-Jiang Zhong
Keyword(s):  
Adventitious Roots ◽  
Large Scale ◽  
Large Scale Culture

Microfluidic PDMS (Polydimethylsiloxane) Bioreactor for Large-Scale Culture of Hepatocytes

2004 ◽  
Vol 20 (3) ◽  
pp. 750-755 ◽  
Author(s):  
E. Leclerc ◽  
Y. Sakai ◽  
T. Fujii
Keyword(s):  
Large Scale ◽  
Large Scale Culture ◽  
Culture Of Hepatocytes

Large-Scale Culture of Vero Cells on GT-2 Microcarrier in Cell Culture Bioreactors

1992 ◽  
pp. 347-349
Author(s):  
Chen Yinliang ◽  
Dong Shupei ◽  
Gu Xiaohua ◽  
Yan Chun ◽  
Song Jiali ◽  
...  
Keyword(s):  
Cell Culture ◽  
Large Scale ◽  
Vero Cells ◽  
Large Scale Culture

Large Scale Culture of Pluripotent Stem Cells for Therapy

Stem Cells ◽  
2010 ◽  
pp. 127-154
Author(s):  
Mahendra Rao ◽  
Mohan C. Vemuri
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Large Scale ◽  
Large Scale Culture

scholarly journals Large-Scale Microcarrier Culture of Chinese Perch Brain Cell for Viral Vaccine Production in a Stirred Bioreactor

Vaccines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1003
Author(s):  
Xia Luo ◽  
Yinjie Niu ◽  
Xiaozhe Fu ◽  
Qiang Lin ◽  
Hongru Liang ◽  
...  
Keyword(s):  
Culture System ◽  
Large Scale ◽  
Brain Cell ◽  
Vaccine Production ◽  
Mandarin Fish ◽  
Siniperca Chuatsi ◽  
Stirred Bioreactor ◽  
Viral Vaccine ◽  
Chinese Perch ◽  
Large Scale Culture

Mandarin fish (Siniperca chuatsi) is one of the important cultured fish species in China. Infectious spleen and kidney necrosis virus (ISKNV) and Siniperca Chuatsi rhabdovirus (SCRV) have hindered the development of mandarin fish farming industry. Vaccination is the most effective method for control of viral diseases, however viral vaccine production requires the large-scale culture of cells. Herein, a suspension culture system of Chinese perch brain cell (CPB) was developed on Cytodex 1 microcarrier in a stirred bioreactor. Firstly, CPB cells were cultured using Cytodex 1 microcarrier in 125 mL stirring flasks. With the optimum operational parameters, CPB cells grew well, distributed uniformly, and could fully cover the microcarriers. Then, CPB cells were digested with trypsin and expanded step-by-step with different expansion ratios from the 125 mL stirring bottle to a 500 mL stirring bottle, and finally to a 3-L bioreactor. Results showed that with an expansion ratio of 1:3, we achieved a high cell density level (2.25 × 106 cells/mL) with an efficient use of the microcarriers, which also confirmed the data obtained from the 125 mL stirring flask. Moreover, obvious cytopathic effects (CPE) were observed in the suspended CPB cells post-infection with ISKNV and SCRV. This study provided a large-scale culture system of CPB cells for virus vaccine production.

scholarly journals Development of a modular automated system for maintenance and differentiation of adherent human pluripotent stem cells

2016 ◽  
Author(s):  
Duncan E. Crombie ◽  
Maciej Daniszewski ◽  
Helena H. Liang ◽  
Tejal Kulkarni ◽  
Fan Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Large Scale ◽  
Human Pluripotent Stem Cells ◽  
Automated System ◽  
Patient Specific ◽  
High Throughput Analysis ◽  
Large Scale Culture ◽  
Induced Pluripotent ◽  
Selection Of

AbstractPatient-specific induced pluripotent stem cells (iPSCs) have tremendous potential for development of regenerative medicine, disease modelling and drug discovery. However, the processes of reprogramming, maintenance and differentiation are labour intensive and subject to inter-technician variability. To address these issues, we established and optimised protocols to allow for the automated maintenance of reprogrammed somatic cells into iPSCs to enable the large-scale culture and passaging of human pluripotent stem cells (PSCs) using a customized TECAN Freedom EVO. Generation of iPSCs was performed offline by nucleofection followed by selection of TRA-1-60 positive cells using a Miltenyi MultiMACS24 Separator. Pluripotency markers were assessed to confirm pluripotency of the generated iPSCs. Passaging was performed using an enzyme-free dissociation method. Proof of concept of differentiation was obtained by differentiating human PSCs into cells of the retinal lineage. Key advantages of this automated approach are the ability to increase sample size, reduce variability during reprogramming or differentiation, and enable medium to high-throughput analysis of human PSCs and derivatives. These techniques will become increasingly important with the emergence of clinical trials using stem cells.

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