Cholinergic Neurons of the Rat Forebrain in Slice Cultures; Interactions with Target Tissue and Effects of Nerve Growth Factor

1988 ◽  
pp. 81-92 ◽  
Author(s):  
F. Hefti ◽  
B. H. Gähwiler
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Cholinergic Neurons ◽  
Target Tissue ◽  
Nerve Growth ◽  
Rat Forebrain

scholarly journals Intermittent vs Continuous Administration of Nerve Growth Factor to Injured Medial Septal Cholinergic Neurons in Rat Basal Forebrain

2014 ◽  
Vol 05 (02) ◽  
pp. 109-118
Author(s):  
Kenneth E. Miller ◽  
Gregory E. Frierdich ◽  
Robert H. Dillard ◽  
Robert H. Soriano ◽  
Dikla G. Roufa
Keyword(s):  
Nerve Growth Factor ◽  
Growth Factor ◽  
Basal Forebrain ◽  
Cholinergic Neurons ◽  
Continuous Administration ◽  
Nerve Growth

TF-1 Cell Proliferation Assay Method for Estimating Bioactivity of Nerve Growth Factor

2013 ◽  
Vol 2 (2) ◽  
pp. 32
Author(s):  
Lingyan Ma ◽  
Yufei Zou
Keyword(s):  
Biological Activity ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Mtt Assay ◽  
Cholinergic Neurons ◽  
Assay Method ◽  
Test Substance ◽  
Nerve Growth ◽  
Standard Curve ◽  
Plate Reader

<p>Nerve growth factor (NGF) is a neurotrophic factor responsible for the differentiation and survival of sympathetic and sensory neurons as well as selective populations of cholinergic neurons. The MTT assay is to add the chromogenic substance MTT after the NGF has stimulated the TF1 cells, to conduct a colorimetric analysis on the micro plate reader, then to compare the results with a standard curve to arrive at the biological activity of the test substance. To use the MTT assay to measure the biological activity of the NGF, it is easy to handle, with good and reliable results, repeatable and with no impact of the human factors.</p>

Sign in / Sign up

Export Citation Format

Share Document