Organelle DNA Isolation

1997 ◽  
pp. 281-304
Author(s):  
M. Landgren ◽  
K. Glimelius
Keyword(s):  
Dna Isolation ◽  
Organelle Dna

scholarly journals Detection of Peanut Allergen by Real Time PCR: Looking For the Suitable Detection Marker as Affected by Processing

2021 ◽  
Author(s):  
Africa Sanchiz ◽  
Paulina Sánchez-Enciso ◽  
Carmen Cuadrado ◽  
Rosario Linacero
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Dna Isolation ◽  
Peanut Flour ◽  
Coding Region ◽  
Peanut Allergen ◽  
Assay Sensitivity ◽  
Organelle Dna ◽  
Chloroplast Markers ◽  
Specificity And Sensitivity

Peanut (Arachis hypogaea) contains allergenic proteins, which make it harmful to the sensitised population. The presence of peanut in foods must be indicated on label, to prevent accidental consumption by allergic population.. In this work, we use chloroplast markers for specifically detection of peanut by real-time PCR, in order to increase the assay sensitivity. Three different protocols of DNA isolation were evaluated, for total and organelle-DNA extraction. Binary mixtures of raw and processed peanut flour in wheat were performed at concentrations ranging from 100000 to 0.1 mg/kg. DNA isolation from peanut, mixtures and other legumes was carried out following three protocols for obtaining genomic and chloroplast-enrich DNA. Quantity and quality of DNA was evaluated, obtaining better results for protocol 2. Specificity and sensitivity of the method has been assayed with specific primers for three chloroplast markers (mat k, rpl16 and trnH-psbA) and Ara h 6 peanut allergen-coding region was selected as nuclear low-copy target and TaqMan probes. Efficiency and linear correlation of calibration curves were within the adequate ranges. Moreover, the influence of pressure and thermal processing on the peanut detectability was analyzed.

Genomic Dna Isolation From Plumbago L. Species Growing In India

2012 ◽  
Vol 2 (7) ◽  
pp. 45-46
Author(s):  
Varsha N Nathar ◽  
◽  
Prashant J Gadge
Keyword(s):  
Genomic Dna ◽  
Dna Isolation ◽  
Genomic Dna Isolation

Extraction of DNA from a Wide Range of Objects by Treatment with Ammonium Salts

2020 ◽  
Vol 36 (6) ◽  
pp. 98-106
Author(s):  
E.I. Levitin ◽  
B.V. Sviridov ◽  
O.V. Piksasova ◽  
T.E. Shustikova
Keyword(s):  
Dna Extraction ◽  
Dna Isolation ◽  
Tissue Samples ◽  
New Approach ◽  
Cell Wall Disruption ◽  
Wide Range ◽  
Low Salt ◽  
Mammalian Blood ◽  
Plasmid Extraction ◽  
Two Hybrid

Currently, simple, rapid, and efficient techniques for DNA isolation from a wide range of organisms are in demand in biotechnology and bioinformatics. A key (and often limiting) step is the cell wall disruption and subsequent DNA extraction from the disintegrated cells. We have developed a new approach to DNA isolation from organisms with robust cell walls. The protocol includes the following steps: treatment of cells or tissue samples with ammonium acetate followed by cell lysis in low-salt buffer with the addition of SDS. Further DNA extraction is carried out according to standard methods. This approach is efficient for high-molecular native DNA isolation from bacteria, ascomycetes, yeast, and mammalian blood; it is also useful for express analysis of environmental microbial isolates and for plasmid extraction for two-hybrid library screening. express method for DNA isolation; ammonium salt treatment (в русских ключевых такой порядок), osmotic breakage of cells This study was financially supported by the NRC "Kurchatov Institute"-GOSNIIGENETIKA Kurchatov Genomic Center.

scholarly journals Application of Doyle and Doyle method for DNA isolation from pamelo yellow orange (Citrus maxima Merr), lime orange (C. limon) and sunkist orange (C. sinensis)

2021 ◽  
Vol 1943 (1) ◽  
pp. 012079
Author(s):  
S N Mawarni ◽  
D Khairunnisa ◽  
I Larasati ◽  
N Rizqo ◽  
T Erfianti ◽  
...  
Keyword(s):  
Dna Isolation ◽  
Yellow Orange ◽  
Citrus Maxima

scholarly journals Tissue-specific organelle DNA degradation mediated by DPD1 exonuclease

2011 ◽  
Vol 6 (9) ◽  
pp. 1391-1393 ◽  
Author(s):  
Lay Yin Tang ◽  
Wataru Sakamoto
Keyword(s):  
Dna Degradation ◽  
Tissue Specific ◽  
Organelle Dna

scholarly journals Evaluation of rapid protocols for DNA isolation from Cercospora beticola Sacc.

2012 ◽  
Vol 49 (2) ◽  
pp. 202-207
Author(s):  
Budakov Dragana ◽  
Nagl Nevena ◽  
Taski-Ajdukovic Ksenija ◽  
Stojsin Vera ◽  
Bagi Ferenc
Keyword(s):  
Dna Isolation ◽  
Cercospora Beticola
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