scholarly journals Fetal Hematopoietic Stem Cells: In Vitro Expansion and Transduction Using Lentiviral Vectors

2001 ◽  
pp. 123-144
Author(s):  
A. Luther-Wyrsch ◽  
C. Nissen ◽  
D. V. Surbek ◽  
W. Holzgreve ◽  
E. Costello ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Lentiviral Vectors ◽  
Hematopoietic Stem ◽  
In Vitro Expansion

In vitro expansion of long-term repopulating hematopoietic stem cells in the presence of immobilized Jagged-1 and early acting cytokines

2006 ◽  
Vol 30 (5) ◽  
pp. 401-405 ◽  
Author(s):  
Z KERTESZ ◽  
V VAS ◽  
J KISS ◽  
V URBAN ◽  
E POZSONYI ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Hematopoietic Stem ◽  
In Vitro Expansion

In Vitro Expansion of Hematopoietic Stem Cells by Inhibition of Both GSK3 and p38 Signaling

2019 ◽  
Vol 28 (22) ◽  
pp. 1486-1497 ◽  
Author(s):  
Jing Li ◽  
Lei Zhang ◽  
Lizhi Yin ◽  
Na Ma ◽  
Tian Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Hematopoietic Stem ◽  
In Vitro Expansion

Adrenomedullin and endothelin-1 stimulate in vitro expansion of cord blood hematopoietic stem cells

2004 ◽  
Author(s):  
Sergio De Angeli ◽  
Laura Del Pup ◽  
Erica Febas ◽  
Maria Conconi ◽  
Mara Tommasini ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Cord Blood ◽  
Hematopoietic Stem ◽  
Endothelin 1 ◽  
In Vitro Expansion

scholarly journals In Vitro Expansion of Hematopoietic Progenitors and Maintenance of Stem Cells: Comparison Between FLT3/FLK-2 Ligand and KIT Ligand

Blood ◽  
1997 ◽  
Vol 89 (6) ◽  
pp. 1915-1921 ◽  
Author(s):  
Yuji Yonemura ◽  
Hsun Ku ◽  
Stewart D. Lyman ◽  
Makio Ogawa
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Cultured Cells ◽  
Rational Approach ◽  
Hematopoietic Stem ◽  
Kit Ligand ◽  
In Vitro Expansion ◽  
Cytokine Combination ◽  
Marrow Cells

Abstract The effects of FLT3/FLK-2 ligand (FL) and KIT ligand (KL) on in vitro expansion of hematopoietic stem cells were studied using lineage-negative (Lin−)Sca-1–positive (Sca-1+) c-kit–positive (c-kit+) marrow cells from 5-fluorouracil (5-FU)–treated mice. As single agents, neither FL nor KL could effectively support the proliferation of enriched cells in suspension culture. However, in combination with interleukin-11 (IL-11), both FL and KL enhanced the production of nucleated cells and progenitors. The kinetics of stimulation by FL was different from that by KL in that the maximal expansion by FL of the nucleated cell and progenitor pools required a longer incubation than with KL. We then tested the reconstituting abilities of cells cultured for 1, 2, and 3 weeks by transplanting the expanded Ly5.1 cells together with “compromised” marrow cells into lethally irradiated Ly5.2 mice. Cells that had been expanded with either cytokine combination were able to maintain the reconstituting ability of the original cells. Only cells that had been incubated with KL and IL-11 for 21 days had less reconstituting ability than fresh marrow cells. These results indicate that there can be significant expansion of progenitors in vitro without compromising the reconstituting ability of stem cells. Addition of IL-3 to permissive cytokine combinations significantly reduced the ability of cultured cells to reconstitute the hematopoiesis of irradiated hosts. These observations should provide a basis for a rational approach to designing cytokine combinations for in vitro expansion of hematopoietic stem cells.

Isolation and cultivation of murine hematopoietic stem cells and expression of hFIX mediated by recombinant lentiviral vectors in vitro

2006 ◽  
Vol 1 (3) ◽  
pp. 259-262
Author(s):  
Hengmei Yao ◽  
Haoming Chen ◽  
Lu Huang ◽  
Qi Shen ◽  
Weiguo Jia ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Lentiviral Vectors ◽  
Hematopoietic Stem

In vitro expansion of hematopoietic stem cells by recombinant TAT-HOXB4 protein

10.1038/nm951 ◽  
2003 ◽  
Vol 9 (11) ◽  
pp. 1428-1432 ◽  
Author(s):  
Jana Krosl ◽  
Pamela Austin ◽  
Nathalie Beslu ◽  
Evert Kroon ◽  
R Keith Humphries ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Hematopoietic Stem ◽  
In Vitro Expansion
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