Three-Dimensional Super-Resolving Confocal Scanning Laser Fluorescent Microscopy

1997 ◽  
pp. 1-6
Author(s):  
Ibrahim Akduman ◽  
Jan Grochmalicki ◽  
Roy Pike
Keyword(s):  
Fluorescent Microscopy ◽  
Three Dimensional ◽  
Scanning Laser ◽  
Confocal Scanning ◽  
Confocal Scanning Laser

Confocal Image Properties of a Confocal Scanning Laser Visible Light FT-Raman Microscope

1995 ◽  
Vol 49 (7) ◽  
pp. 971-976 ◽  
Author(s):  
Colin J. H. Brenan ◽  
Ian W. Hunter
Keyword(s):  
Visible Light ◽  
Three Dimensional ◽  
Depth Resolution ◽  
Scanning Laser ◽  
Chemical Structures ◽  
Confocal Scanning ◽  
Confocal Raman ◽  
Confocal Scanning Laser ◽  
Imaging Properties ◽  
Ft Raman

The confocal Raman microscope is an instrument designed for acquisition of high-contrast volumetric Raman spectral images of three-dimensional chemical structures. Little effort, however, has gone into the investigation of the spatial imaging properties of this class of confocal microscope. In this paper we present experimental results, obtained with a confocal scanning laser visible light FT-Raman microscope we built, that demonstrates the high depth resolution and enhanced Raman image contrast intrinsic to the confocal Raman microscope design. We explore these microscope properties through a combination of experimental measurement and theory based on a paraxial wave diffraction model.

Three-dimensional images of DAB-labeled neurons by confocal scanning laser microscopy

1989 ◽  
Vol 47 ◽  
pp. 144-145
Author(s):  
JS Deitch ◽  
KL Smith ◽  
C Lee ◽  
JW Swann ◽  
JN Turner
Keyword(s):  
Three Dimensional ◽  
Neuronal Morphology ◽  
Scanning Laser ◽  
Confocal Scanning Laser Microscopy ◽  
Confocal Scanning Laser Microscope ◽  
Basal Dendrites ◽  
Confocal Scanning ◽  
Three Dimensional Images ◽  
Confocal Scanning Laser ◽  
Areas Of Interest

The ability to correlate neuronal morphology and physiology has been greatly advanced by intracellular labeling through the recording pipette. However, visualizing the filled neuron required physically sectioning and reconstructing areas of interest, often resulting in figures that are two-dimensional. We have visualized the three-dimensional morphology of filled neurons reacted with nickel-intensified diaminobenzidine (DAB/Ni) using the confocal scanning laser microscope (CSLM).Neurons in slices of rat hippocampus were filled with biocytin, fixed in 4% paraformaldehyde, incubated in avidin-HRP (1:200) in 0.5% Triton X-100, and reacted with DAB in 0.04% nickel ammonium sulfate. Optical sections and three-dimensional images were recorded by using a Bio Rad MRC-500 CSLM with an argon ion laser.Biocytin filled all aspects of the neuron, including fine axons and spines. Fig. 1 is a conventional micrograph of a single neuron labeled with DAB/Ni. Figs. 2a and b are stereo pairs of the apical and basal dendrites of the neuron in Fig. 1.

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