The Tricarboxylic Acid Cycle and the Glyoxylate Bypass

2010 ◽  
pp. 79-99 ◽  
Author(s):  
G. N. Cohen
Keyword(s):  
Tricarboxylic Acid Cycle ◽  
Tricarboxylic Acid ◽  
Glyoxylate Bypass ◽  
Acid Cycle

Tricarboxylic Acid Cycle and Glyoxylate Bypass

EcoSal Plus ◽  
2005 ◽  
Vol 1 (2) ◽  
Author(s):  
John E. Cronan, Jr. ◽  
David Laporte
Keyword(s):  
Tricarboxylic Acid Cycle ◽  
Tricarboxylic Acid ◽  
Glyoxylate Bypass ◽  
Acid Cycle

INTERRELATIONSHIPS BETWEEN THE TRICARBOXYLIC ACID AND GLYOXYLATE CYCLES STUDIED WITH BACTERIAL AUXOTROPHS

1962 ◽  
Vol 8 (2) ◽  
pp. 241-247 ◽  
Author(s):  
Henry C. Reeves ◽  
Samuel J. Ajl
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Tricarboxylic Acid Cycle ◽  
Tricarboxylic Acid ◽  
Mineral Salts ◽  
Glyoxylate Bypass ◽  
Acid Cycle ◽  
E Coli ◽  
Acetate Medium ◽  
Metabolic Significance

An autotroph of Escherichia coli, E26-6, which is unable to grow aerobically in a simple mineral-salts medium with either acetate, glutamate, isocitrate, or any one of the C4 dicarboxylic acid intermediates of the tricarboxylic acid cycle as sole carbon source, has been investigated. The mutant is able to grow, however, in a mineral-salts acetate medium supplemented with any one of the above acids. The specific activities of the tricarboxylic acid cycle and glyoxylate bypass enzymes, with the exception of alpha-ketoglutaric dehydrogenase, which is greatly impaired in the auxotroph, were found to be essentially the same in both the parent and the mutant. Thus, the glyoxylate bypass alone is not capable of supplying sufficient C4 intermediates to allow the growth of E. coli on acetate. Further, there appear to be no other metabolic pathways leading to C4 production, which are of major metabolic significance during growth on acetate, other than the tricarboxylic and glyoxylate cycles. Finally, in conjunction with the tricarboxylic acid cycle, the malate synthetase and isocitritase reactions provide a mechanism which enables E. coli to grow on a medium containing acetate as the sole carbon source.

Biosynthesis of ε-rhodomycinone from glucose by Streptomyces C5 and comparison with intermediary metabolism of other polyketide-producing streptomycetes

1988 ◽  
Vol 34 (11) ◽  
pp. 1235-1240 ◽  
Author(s):  
Michael L. Dekleva ◽  
William R. Strohl
Keyword(s):  
Pentose Phosphate Pathway ◽  
Enzyme Activities ◽  
Tricarboxylic Acid Cycle ◽  
Tricarboxylic Acid ◽  
Pentose Phosphate ◽  
Anthracycline Antibiotics ◽  
Glyoxylate Bypass ◽  
Acid Cycle ◽  
Minor Contribution ◽  
A Minor

The catabolism of glucose by Streptomyces C5, a producer of anthracycline antibiotics, was investigated to determine the pathways that supply precursors for anthracycline biosynthesis. Carbons for the biosynthesis of ε-rhodomycinone, an anthracycline aglycone, from radiolabelled glucose were derived primarily from the Embden–Meyerhof–Parnas pathway, with a minor contribution from the pentose phosphate pathway. Furthermore, the anthracycline-producing strain, Streptomyces C5, as well as Streptomyces aureofaciens and Streptomyces lividans, strains that produce nonanthracycline polyketide antibiotics, displayed enzyme activities indicative of the Embden–Meyerhof–Parnas and pentose phosphate glycolytic pathways. As determined from labelling patterns, Streptomyces C5 apparently has a complete tricarboxylic acid cycle, but does not have a glyoxylate bypass pathway.

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