Plant Regeneration from Embryogenic Calli, Cell Suspension Cultures and Protoplasts of Triticum aestivum L. (Wheat)

1990 ◽  
pp. 33-37 ◽  
Author(s):  
Indra K. Vasil ◽  
Vimla Vasil ◽  
Flona Redway
Keyword(s):  
Triticum Aestivum ◽  
Plant Regeneration ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Triticum Aestivum L ◽  
Suspension Cultures ◽  
Embryogenic Calli

Uptake, Translocation, and Metabolism of 3,4-Dichloroaniline in Soybean and Wheat Plants

1994 ◽  
Vol 49 (11-12) ◽  
pp. 719-726 ◽  
Author(s):  
Margret Bockers ◽  
Christiane Rivero ◽  
Brigitte Thiede ◽  
Thomas Jankowski ◽  
Burkhard Schmidt
Keyword(s):  
Triticum Aestivum ◽  
Glycine Max ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Triticum Aestivum L ◽  
Suspension Cultures ◽  
Main Metabolite ◽  
Wheat Roots ◽  
Glycine Max L ◽  
Soybean Plants

The roots of 13-day-old soybean ( Glycine max L.) and 7-day-old wheat ( Triticum aestivum L.) hydroponic plants were exposed to [14C]-3,4-dichloroaniline (1.0 and 0.4 mg/1 (6.2 and 2.5 μᴍ) , respectively) and harvested after 48/120 h (soybean) and 72 h (wheat). Both species metabolized the xenobiotic almost quantitatively to N-(β-D-glucopyranosyl)-3,4-dichloroaniline, N-malonyl-3,4-dichloroaniline, 6′-O-malonyl-N-(β-D-glucopyranosyl)-3,4-dichloroaniline and non-extractable residues. In the soybean experiments 58.8 (48 h) and 54.6% (120 h) of the applied radioactivity were found in the nutrient; this fraction consisted primarily of N-malonyl-3,4-dichloroaniline. 37.3/24.1% (48/120 h) were detected in the plants. In the soybeans, 36.2 and 52.7% (48/120 h) of the absorbed 14C were translocated, mainly into hypocotyls, primary and secondary leaves. After 120 h, the main metabolite was N-malonyl- 3,4-dichloroaniline (38.5% ); considerable levels of this metabolite accumulated in the primary and secondary leaves (10.4 and 10.4%). The glucosides were mainly found in the roots of the soybean plants. Totals of 23.5 and 35.1% (48/120 h) were transformed to non-extractable residues. In wheat, 78.3% of the applied 3,4-dichloroaniline was absorbed after 72 h. This fraction was partially translocated to the leaves, but most of the residues remained in the roots (90.3% of absorbed 14C). In wheat, a total of 45.6% was transformed to nonextractable residues. The soluble radioactivity in the roots consisted of nearly equal amounts of the glucosides and the N-malonyl conjugate. The processes observed in soybean and wheat roots resembled those of the respective cell suspension cultures published previously.

Characterization and plant regeneration of cell suspension cultures of Lycopersicon chilense

1991 ◽  
Vol 69 (10) ◽  
pp. 2257-2260 ◽  
Author(s):  
Ann Francine Greer ◽  
Zohreh Tabaeizadeh
Keyword(s):  
Plant Regeneration ◽  
Cell Suspension ◽  
Casein Hydrolysate ◽  
Leaf Explants ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Cell Suspensions ◽  
Lag Phase ◽  
Petiole Explants ◽  
Lycopersicon Chilense

To produce calli for the establishment of a cell suspension, leaf, stem, and petiole explants of Lycopersicon chilense Dun., grown in vitro and in the soil, were cultured on media containing 15 different combinations of benzylaminopurine, kinetin, and indole acetic acid. Among the three types of tissues, leaf explants showed the best response. Cell suspension cultures of L. chilense were established from leaf callus derived from soil grown plants using Murashige and Skoog's medium supplemented with casein hydrolysate (250 mg/L), coconut water (5%), and 2,4-dichlorophenoxyacetic acid (2 mg/L). Once established, cell suspensions showed a rapid growth rate with no marked lag phase. Shooting via organogenesis occurred from callus derived from cell suspensions on medium containing 2 mg/L benzylaminopurine. Regenerated plants had the same morphology as the original plants. Key words: Lycopersicon chilense, tomato, tissue culture, cell suspensions, organogenesis, plant regeneration.

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