Characterization and plant regeneration of cell suspension cultures of Lycopersicon chilense

1991 ◽  
Vol 69 (10) ◽  
pp. 2257-2260 ◽  
Author(s):  
Ann Francine Greer ◽  
Zohreh Tabaeizadeh
Keyword(s):  
Plant Regeneration ◽  
Cell Suspension ◽  
Casein Hydrolysate ◽  
Leaf Explants ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Cell Suspensions ◽  
Lag Phase ◽  
Petiole Explants ◽  
Lycopersicon Chilense

To produce calli for the establishment of a cell suspension, leaf, stem, and petiole explants of Lycopersicon chilense Dun., grown in vitro and in the soil, were cultured on media containing 15 different combinations of benzylaminopurine, kinetin, and indole acetic acid. Among the three types of tissues, leaf explants showed the best response. Cell suspension cultures of L. chilense were established from leaf callus derived from soil grown plants using Murashige and Skoog's medium supplemented with casein hydrolysate (250 mg/L), coconut water (5%), and 2,4-dichlorophenoxyacetic acid (2 mg/L). Once established, cell suspensions showed a rapid growth rate with no marked lag phase. Shooting via organogenesis occurred from callus derived from cell suspensions on medium containing 2 mg/L benzylaminopurine. Regenerated plants had the same morphology as the original plants. Key words: Lycopersicon chilense, tomato, tissue culture, cell suspensions, organogenesis, plant regeneration.

High-frequency plant regeneration from embryogenic cell suspension cultures of Gynura procumbens

2018 ◽  
Vol 13 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Eun Yee Jie ◽  
Nornita Sham Atong ◽  
Woo Seok Ahn ◽  
Myung Suk Ahn ◽  
Byung Hwan Min ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Cell Suspension ◽  
High Frequency ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Embryogenic Cell Suspension ◽  
Embryogenic Cell

Major Indole Alkaloids Produced in Cell Suspension Cultures of Rhazya strict a Decaisne

1986 ◽  
Vol 41 (4) ◽  
pp. 385-390 ◽  
Author(s):  
Karl-Heinz Pawelka ◽  
Joachim Stöckigt
Keyword(s):  
Cell Suspension ◽  
Indole Alkaloids ◽  
Cell Suspension Cultures ◽  
Structural Heterogeneity ◽  
Suspension Cultures ◽  
Cell Suspensions ◽  
Minor Components ◽  
Rhazya Stricta ◽  
First Time

Eleven main alkaloids were identified from cell suspension cultures of Rhazya stricta grown in 4X-medium for 15 days. The alkaloids comprised the five groups Corynanthe, Strychnos, Eburnan, Secodine, and Aspidosperma and can be regarded as being typical Rhazya alkaloids, although the Strychnos alkaloid akuammicine has been isolated for the first time from the genus Rhazya. The most abundant compound was ( + )-1,2-dehydroaspidospermidine (15 mg/l medium) whereas all other constituents were synthesized in about 5 - 10 times lower amounts. More than 15 further alkaloids were formed as minor components which have not yet been identified. Cultivated Rhazya cells have been shown to be one of the richest alkaloid sources from apocynaceous cell suspensions. The isolated compounds, because of their structural heterogeneity, cannot be presently arranged into a scheme with coherent biosynthetic sequences.

Establishment of Callus and Cell Suspension Cultures of Corydalis saxicola Bunting, a Rare Medicinal Plant

2006 ◽  
Vol 61 (3-4) ◽  
pp. 251-256 ◽  
Author(s):  
Hua Cheng ◽  
Long-Jiang Yu ◽  
Qiong-Yue Hu ◽  
Shan-Cai Chen ◽  
You-Ping Sun
Keyword(s):  
Cell Suspension ◽  
Callus Induction ◽  
Large Scale ◽  
Callus Formation ◽  
Leaf Explants ◽  
Cell Suspension Cultures ◽  
Biomass Accumulation ◽  
Suspension Cultures ◽  
Inoculum Size ◽  
Enhanced Production

An efficient procedure has been developed for callus induction and cell suspension cultures of C. saxicola for the first time. Explant selection was carried out among leaf, stem and root to select a suitable type of explants capable of higher callus formation. Leaf explants thus selected showed maximum response to callus induction (67.1%). Modified B5 medium supplemented with 0.5 mg l−1 2,4-D plus 2 mg l−1 BA was the most favorable medium for callus formation with the highest induction rate (94.8%) and greatest fresh weight of callus (1.7 g per explant). Cell suspension cultures were established by transferring 2-8 g fresh callus to 80 ml liquid B5 medium. An inoculum size of 8 g produced the greatest biomass accumulation, dehydrocavidine and berberine productions, which was 13.1 g l−1, 8.0 mg l−1 and 4.1 mg l−1, respectively. In response to various sucrose concentrations from 10 g l−1 to 80 g l−1, cultures with 60 g sucrose l−1 not only produced the highest dry biomass (18.5 g l−1) but also the highest formation of dehydrocavidine (11.6 mg l−1) and berberine (7.6 mg l−1). These prepared cell suspension cultures provided a useful material for further regulation of alkaloid biosynthesis and for enhanced production of valuable alkaloids on a large scale.

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