Metabolism of pentachlorophenol in cell suspension cultures of wheat (Triticum aestivum L.). Tetrachlorocatechol as a primary metabolite

1988 ◽  
Vol 36 (2) ◽  
pp. 370-377 ◽  
Author(s):  
Wolfgang Schaefer ◽  
Heinrich Sandermann
Keyword(s):  
Triticum Aestivum ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Triticum Aestivum L ◽  
Suspension Cultures ◽  
Primary Metabolite

Uptake, Translocation, and Metabolism of 3,4-Dichloroaniline in Soybean and Wheat Plants

1994 ◽  
Vol 49 (11-12) ◽  
pp. 719-726 ◽  
Author(s):  
Margret Bockers ◽  
Christiane Rivero ◽  
Brigitte Thiede ◽  
Thomas Jankowski ◽  
Burkhard Schmidt
Keyword(s):  
Triticum Aestivum ◽  
Glycine Max ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Triticum Aestivum L ◽  
Suspension Cultures ◽  
Main Metabolite ◽  
Wheat Roots ◽  
Glycine Max L ◽  
Soybean Plants

The roots of 13-day-old soybean ( Glycine max L.) and 7-day-old wheat ( Triticum aestivum L.) hydroponic plants were exposed to [14C]-3,4-dichloroaniline (1.0 and 0.4 mg/1 (6.2 and 2.5 μᴍ) , respectively) and harvested after 48/120 h (soybean) and 72 h (wheat). Both species metabolized the xenobiotic almost quantitatively to N-(β-D-glucopyranosyl)-3,4-dichloroaniline, N-malonyl-3,4-dichloroaniline, 6′-O-malonyl-N-(β-D-glucopyranosyl)-3,4-dichloroaniline and non-extractable residues. In the soybean experiments 58.8 (48 h) and 54.6% (120 h) of the applied radioactivity were found in the nutrient; this fraction consisted primarily of N-malonyl-3,4-dichloroaniline. 37.3/24.1% (48/120 h) were detected in the plants. In the soybeans, 36.2 and 52.7% (48/120 h) of the absorbed 14C were translocated, mainly into hypocotyls, primary and secondary leaves. After 120 h, the main metabolite was N-malonyl- 3,4-dichloroaniline (38.5% ); considerable levels of this metabolite accumulated in the primary and secondary leaves (10.4 and 10.4%). The glucosides were mainly found in the roots of the soybean plants. Totals of 23.5 and 35.1% (48/120 h) were transformed to non-extractable residues. In wheat, 78.3% of the applied 3,4-dichloroaniline was absorbed after 72 h. This fraction was partially translocated to the leaves, but most of the residues remained in the roots (90.3% of absorbed 14C). In wheat, a total of 45.6% was transformed to nonextractable residues. The soluble radioactivity in the roots consisted of nearly equal amounts of the glucosides and the N-malonyl conjugate. The processes observed in soybean and wheat roots resembled those of the respective cell suspension cultures published previously.

Metabolic Fingerprinting and Profiling of Arabidopsis thaliana Leaf and its Cultured Cells T87 by GC/MS

2006 ◽  
Vol 61 (3-4) ◽  
pp. 267-272 ◽  
Author(s):  
Eiichiro Fukusaki ◽  
Kanokwan Jumtee ◽  
Takeshi Bamba ◽  
Takehiro Yamaji ◽  
Akio Kobayashi
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Suspension ◽  
Cultured Cells ◽  
Cell Suspension Cultures ◽  
Metabolite Profile ◽  
Culture Cell ◽  
Suspension Cultures ◽  
Primary Metabolite ◽  
Primary Metabolites ◽  
Suspension Culture Cell

Cell suspension cultures are now recognized as important model materials for plant bioscience and biotechnology. Very few studies of metabolic comparisons between cell cultures and original plants have been reported, even though the biological identity of cultured cells with the normally grown plant is of great importance. In this study, a comparison of the metabolome for primary metabolites extracted from the leaves of Arabidopsis thaliana and cultured cells from an Arabidopsis suspension culture (cell line T87) was performed. The results suggest that although cell suspension cultures and Arabidopsis leaves showed similarities in the common primary metabolite profile, nonetheless, moderate differences in quantitative profile were revealed.

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