Detection of viable Yersinia pestis by fluorescence in situ hybridization using peptide nucleic acid probes

2008 ◽  
Vol 75 (2) ◽  
pp. 293-301 ◽  
Author(s):  
John H. Kenny ◽  
Yan Zhou ◽  
Martin E. Schriefer ◽  
Scott W. Bearden
Keyword(s):  
In Situ Hybridization ◽  
Nucleic Acid ◽  
Fluorescence In Situ Hybridization ◽  
Yersinia Pestis ◽  
Peptide Nucleic Acid ◽  
Nucleic Acid Probes

scholarly journals Fluorescence in situ Hybridization method using Peptide Nucleic Acid probes for rapid detection of Lactobacillus and Gardnerella spp.

2013 ◽  
Vol 13 (1) ◽  
pp. 82 ◽  
Author(s):  
António Machado ◽  
Carina Almeida ◽  
Débora Salgueiro ◽  
Ana Henriques ◽  
Mario Vaneechoutte ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Nucleic Acid ◽  
Fluorescence In Situ Hybridization ◽  
Peptide Nucleic Acid ◽  
Rapid Detection ◽  
Hybridization Method ◽  
Nucleic Acid Probes

scholarly journals Identification of Dekkera bruxellensis(Brettanomyces) from Wine by Fluorescence In Situ Hybridization Using Peptide Nucleic Acid Probes

2001 ◽  
Vol 67 (2) ◽  
pp. 938-941 ◽  
Author(s):  
Henrik Stender ◽  
Cletus Kurtzman ◽  
Jens J. Hyldig-Nielsen ◽  
Ditte Sørensen ◽  
Adam Broomer ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Nucleic Acid ◽  
Fluorescence In Situ Hybridization ◽  
Peptide Nucleic Acid ◽  
Dekkera Bruxellensis ◽  
Specific Sequence ◽  
Nucleic Acid Probes ◽  
Yeast Strains ◽  
Species Specific

ABSTRACT A new fluorescence in situ hybridization method using peptide nucleic acid (PNA) probes for identification ofBrettanomyces is described. The test is based on fluorescein-labeled PNA probes targeting a species-specific sequence of the rRNA of Dekkera bruxellensis. The PNA probes were applied to smears of colonies, and results were interpreted by fluorescence microscopy. The results obtained from testing 127 different yeast strains, including 78 Brettanomycesisolates from wine, show that the spoilage organismBrettanomyces belongs to the species D. bruxellensis and that the new method is able to identifyBrettanomyces (D. bruxellensis) with 100% sensitivity and 100% specificity.

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