High Pressure NMR Studies of Chemical and Biochemical Systems

1993 ◽  
pp. 393-441 ◽  
Author(s):  
Jiri Jonas
Keyword(s):  
High Pressure ◽  
Nmr Studies ◽  
Biochemical Systems

High-Pressure NMR Studies of the Dissociation of Arc Represser and the Cold Denaturation of Ribonuclease A

1996 ◽  
Author(s):  
Xiangdong Peng ◽  
Jerson L. Silva
Keyword(s):  
High Pressure ◽  
High Resolution ◽  
Molten Globule ◽  
2D Nmr ◽  
Ribonuclease A ◽  
Nmr Studies ◽  
Cold Denaturation ◽  
Biochemical Systems ◽  
Nmr Techniques ◽  
Β Sheet

We begin this article with a brief discussion of the specialized high-resolution NMR instrumentation developed for high-pressure studies of biochemical systems. We then present the potential for the unique information content of high-pressure NMR spectroscopy as illustrated by the results of two NMR studies performed recently in our laboratory. Different denatured states of Arc represser are characterized by one-dimensional (1D) and two-dimensional (2D) NMR. Increasing pressure promotes sequential changes in the structure of Arc represser: from the native dimer through a predissociated state to a denaturated molten globule monomer. A compact state (molten globule) of Arc represser is obtained in the dissociation of Arc represser by pressure, whereas high temperature and urea induce dissociation and unfolding to less structured conformations. The presence of NOEs (Nuclear Overhauser Enhancement) in the β-sheet region in the dissociated state suggests that the intersubunit β-sheet (residues 6–14) in the native dimer is replaced by an intramonomer β-sheet. Changes in 2D NMR spectra prior to dissociation indicate the existence of a predissociated state that may represent an intermediate stage in the folding and subunit association pathway of Arc represser. The cold denaturation study of ribonuclease A has shown that high pressure can be utilized not only to perturb the protein structure in a controlled way but also to lower the freezing point of aqueous protein solutions substantially. As a result, one can access subzero temperatures and carry out cold denaturation studies of proteins. The results of the NMR study of the reversible cold denaturation are compared with the heat and pressure denaturation of bovine pancreatic ribonuclease A. High-resolution NMR spectra of complex molecules in the liquid phase usually exhibit a great deal of structure and yield a wealth of information about the molecule. Therefore, it is not surprising that multinuclear high-resolution Fourier transform NMR spectroscopy at high pressure represents the most promising technique in studies of the pressure effects on biochemical systems (Jonas & Jonas, 1994). The high information content of the various advanced NMR techniques, including 2D NMR techniques such as NOESY, COSY, and ROESY, have yet to be fully exploited in high-pressure NMR experiments.

scholarly journals High-Resolution, High-Pressure NMR Studies of Proteins

1998 ◽  
Vol 75 (1) ◽  
pp. 445-452 ◽  
Author(s):  
J. Jonas ◽  
L. Ballard ◽  
D. Nash
Keyword(s):  
High Pressure ◽  
High Resolution ◽  
Nmr Studies

High-Pressure NMR Studies of (Porphinato)iron-Catalyzed Isobutane Oxidation Utilizing Dioxygen as the Stoichiometric Oxidant

1997 ◽  
Vol 119 (7) ◽  
pp. 1791-1792 ◽  
Author(s):  
Kevin T. Moore ◽  
István T. Horváth ◽  
Michael J. Therien
Keyword(s):  
High Pressure ◽  
Nmr Studies

High resolution13C NMR studies of one- and two-dimensional polymerized C60 under high pressure

1997 ◽  
Vol 58 (11) ◽  
pp. 1645-1647 ◽  
Author(s):  
F. Rachdi ◽  
C. Goze ◽  
L. Hajji ◽  
M. Nún˜ez-Regueiro ◽  
L. Marques ◽  
...  
Keyword(s):  
High Pressure ◽  
Two Dimensional ◽  
Nmr Studies
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