Meta-analysis of variable-temperature PCR technique performance for diagnosising Schistosoma japonicum infections in humans in endemic areas

Background As China is moving onto schistosomiasis elimination/eradication, diagnostic methods with both high sensitivity and specificity for Schistosoma japonicum infections in humans are urgently needed. Microscopic identification of eggs in stool is proven to have poor sensitivity in low endemic regions, and antibody tests are unable to distinguish between current and previous infections. Polymerase chain reaction (PCR) technologies for the detection of parasite DNA have been theoretically assumed to show high diagnostic sensitivity and specificity. However, the reported performance of PCR for detecting S. japonicum infection varied greatly among studies. Therefore, we performed a meta-analysis to evaluate the overall diagnostic performance of variable-temperature PCR technologies, based on stool or blood, for detecting S. japonicum infections in humans from endemic areas. Methods We searched literatures in eight electronic databases, published up to 20 January 2021. The heterogeneity and publication bias of included studies were assessed statistically. The risk of bias and applicability of each eligible study were assessed using the Quality Assessment of Diagnostic Accuracy Studies 2 tool (QUADAS-2). The bivariate mixed-effects model was applied to obtain the summary estimates of diagnostic performance. The hierarchical summary receiver operating characteristic (HSROC) curve was applied to visually display the results. Subgroup analyses and multivariate regression were performed to explore the source of heterogeneity. This research was performed following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines and was registered prospectively in PROSPERO (CRD42021233165). Results A total of 2791 papers were retrieved. After assessing for duplications and eligilibity a total of thirteen publications were retained for inclusion. These included eligible data from 4268 participants across sixteen studies. High heterogeneity existed among studies, but no publication bias was found. The pooled analyses of PCR data from all included studies resulted in a sensitivity of 0.91 (95% CI: 0.83 to 0.96), specificity of 0.85 (95% CI: 0.65 to 0.94), positive likelihood ratio of 5.90 (95% CI: 2.40 to 14.60), negative likelihood ratio of 0.10 (95% CI: 0.05 to 0.20) and a diagnostics odds ratio of 58 (95% CI: 19 to 179). Case-control studies showed significantly better performances for PCR diagnostics than cross-sectional studies. This was further evidenced by multivariate analyses. The four types of PCR approaches identified (convention PCR, qPCR, Digital droplet PCR and nested PCR) differed significantly, with nested PCRs showing the best performance. Conclusions Variable-temperature PCR has a satisfactory performance for diagnosing S. japonicum infections in humans in endemic areas. More high quality studies on S. japonicum diagnostic techniques, especially in low endemic areas and for the detection of dual-sex and single-sex infections are required. These will likely need to optimise a nested PCR alongside a highly sensitive gene target. They will contribute to successfully monitoring endemic areas as they move towards the WHO 2030 targets, as well as ultimately helping areas to achieve these goals.

A New Laboratory Technique to Enhance Proppant Consolidation During Propped Hydraulic Fracturing Treatment

The objectives of this investigation were to perform a rock mechanical study to evaluate long term stability of Resin-Coated Proppant (RCP), combined with various additives currently being used in screenless propped hydraulic fracturing completions in the sandstone formations. Thereby providing a tool for the industry to know exactly the duration of the shut-in time before putting a well back onto production. A new experimental method was developed to monitor the curing process of RCP as temperature increases. The velocity of both shear and compressional waves were being monitored as a function of temperature, while the tested RCP sample was being housed in a pressurized vessel. The pressurized vessel was subjected to a variable temperature profile to mimic the recovery of the reservoir temperature following a propped hydraulic fracturing treatment. The placed proppant should attain an optimum consolidation to minimize the potential for proppant flow back. The study has been performed on various types of RCP samples under a range of reservoir conditions. The role of closure stress, temperature, curing time and carrier fluids in attaining a maximum strength of RCP following a propped hydraulic fracturing treatment have been investigated. Also, the Unconfined Compressive Strength (UCS) of various types of RCP have been measured. The testing methods currently practiced in the industry to qualify proppant for field applications are based on physical characterization of several parameters such as the specific gravity of proppant, absolute volume, solubility, roundness, sphericity and bulk density. The sieve analysis, compressive strength, and API crush testing are also measured and reported. The API Recommended Practices; API RP56, API RP58 and API RP60 are the main procedures used to test the suitability of proppants for hydraulic fracturing treatment. However, there is no published API testing method for RCP; therefore this study introduces a new testing procedure, using acoustic velocity as a function of temperature and compressive strength as a function of time; to qualify a given RCP for a particular reservoir of known stress and temperature. The final outcome of this study is to establish a functional procedure for such measurements, in order to maximize the success of a propped hydraulic fracturing treatment and minimize the occurrence of flow back incidents.

Spectral irradiance scale realization and uncertainty analysis based on a 14 mm diameter WC-C fixed point blackbody from 250 nm to 2500 nm

Spectral irradiance scale in the wavelength range from 250 nm to 2500 nm was realized at National Institute of Metrology (NIM) on the basis of a large area tungsten carbide–carbon (WC-C) high temperature fixed point blackbody, which is composed of a 14 mm diameter WC-C fixed point cell and a variable temperature blackbody BB3500MP as a furnace. A series of 1000 W FEL tungsten halogen lamps were used as transfer standards. The new spectral irradiance scale was compared with the scale based on a variable-temperature blackbody BB3500M, and the divergence between these two methods varied from -0.66% to 0.79% from 280 nm to 2100 nm. The measurement uncertainty of spectral irradiance scale based on fixed-point blackbody was analyzed, and the expanded uncertainty was estimated as 3.9% at 250 nm, 1.4% at 280 nm, 0.43 % at 400 nm, 0.27% at 800 nm, 0.25% at 1000 nm, 0.62% at 1500 nm, 0.76% at 2000 nm, and 2.4% at 2500 nm respectively. In the range from 300 nm to 1000 nm the fixed-point scale was improved obviously: the uncertainty decreased by more than 25% compared to the uncertainty based on the variable temperature blackbody. Below 300 nm, the uncertainty became higher because the signal to noise ratio was poor. Above 1100 nm, the contribution of temperature measurement to the uncertainty of spectral irradiance decreases, therefore the uncertainties of two methods are almost at the same level. The fixed-point blackbody was also used to realize the correlated colour temperature and distribution temperature of a tungsten filament lamp, the deviation from the variable temperature blackbody method was -0.5 K and -2.9 K, respectively.

The use of ceramics as an internal curing agent in high performance concrete with variable temperature curing to improve mechanical characteristics

Concrete curing is one of the most significant factors in the development of compressive strength, and a high temperature difference during curing may reduce strength. The microcracks created in the concrete as a result of the constant temperature change cause this exudation. Internal curing has become popular for decreasing the risk of early-age cracking in high-performance concrete by limiting autogenous shrinkage (HPC). This study looks at the effectiveness of internal wet curing offered by a new kind of aggregate called “recycled waste porous ceramic fine aggregates”. The evolution of measured mechanical characteristics is examined on three distinct HPCs, both with and without internal curing materials. Ceramic fine aggregates were used to replace two different quantities of regular weight fine aggregate. Ceramic fine aggregates were shown to be quite beneficial for internal cure. It has been discovered that incorporating 20% ceramic fine aggregates into HPC improves the properties of the material, resulting in low internal stress and a large improvement in compressive strength. It should be emphasized that, unlike some traditional lightweight aggregates, no loss in compressive strength has been seen for the various quantities of ceramic fine aggregates introduced at either early or later ages.

Effect Of Attapulgite as Internal Curing in High-Performance Concrete with Variable Temperature Curing to Enhance Mechanical Properties

One of the most important elements in the development of compressive strength is concrete curing, and a large temperature differential during curing may decrease strength. This exudation is caused by microcracks in the concrete caused by the continuous temperature fluctuation. By minimizing autogenous shrinkage, internal curing has become popular for reducing the danger of early-age cracking in high-performance concrete (HPC). The efficacy of internal wet curing provided by fine Attapulgite aggregate is investigated in this research. On three different HPCs, both with and without internal curing materials, the development of observed mechanical properties is investigated. Two different amounts of normal weight fine aggregate were replaced with attapulgite fine aggregates. Internal cure has been found to benefit from attapulgite fine aggregates. It has been found that adding 20% Attapulgite fine aggregates to HPC enhances the material’s characteristics, resulting in low internal stress and a significant increase in compressive strength. It should be noted that, unlike certain conventional lightweight aggregates, the different amounts of Attapulgite fine aggregates added at various ages have shown no decrease in compressive strength.

Thermal sensitivity and seasonal change in the gut microbiome of a desert ant, Cephalotes rohweri

Microorganisms within ectotherms must withstand the variable body temperatures of their hosts. Shifts in host body temperature resulting from climate change have the potential to shape ectotherm microbiome composition. Microbiome compositional changes occurring in response to temperature in nature have not been frequently examined, restricting our ability to predict microbe-mediated ectotherm responses to climate change. In a set of field-based observations, we characterized gut bacterial communities and thermal exposure across a population of desert arboreal ants (Cephalotes rohweri). In a paired growth chamber experiment, we exposed ant colonies to variable temperature regimes differing by 5 C for three months. We found that the abundance and composition of ant-associated bacteria were sensitive to elevated temperatures in both field and laboratory experiments. We observed a subset of taxa that responded similarly to temperature in the experimental and observational study, suggesting a role of seasonal temperature and local temperature differences amongst nests in shaping microbiomes within the ant population. Bacterial mutualists in the genus Cephalotococcus (Opitutales: Opitutaceae) were especially sensitive to change in temperature - decreasing in abundance in naturally warm summer nests and warm growth chambers. We also report the discovery of a member of the Candidate Phlya Radiation (Phylum: Gracilibacteria), a suspected epibiont, found in low abundance within the guts of this ant species.

Nanocomposites of PCL and SBA-15 Particles Prepared by Extrusion: Structural Characteristics, Confinement of PCL Chains within SBA-15 Nanometric Channels and Mechanical Behavior

A study of different nanocomposites based on poly(ε-caprolactone) (PCL) and mesoporous SBA-15 silica that were prepared by melt extrusion was carried out by analyzing the possible effect of this filler on the crystalline details of PCL, on its mechanical behavior, and on the eventual observation of the confinement of the polymeric chains within the hollow nanometric silica channels. Thus, simultaneous Small-Angle and Wide-Angle X-ray Scattering (SAXS/WAXS) synchrotron experiments at variable temperature were performed on these PCL nanocomposites with different mesoporous silica contents. The importance of the morphological and structural features was assessed by the changes that were observed during the mechanical response of the final materials, which determined that the presence of mesoporous particles leads to a noticeable reinforcing effect.