Photosynthetic Oxygen Evolution in the Filamentous Cyanobacterium Oscillatoria Chalybea: Interrelationship Between Water Splitting, Hydrogen Peroxide Decomposition and Nitrate Metabolism

1991 ◽  
pp. 419-424
Author(s):  
Klaus P. Bader ◽  
Georg H. Schmid
1990 ◽  
Vol 45 (11-12) ◽  
pp. 1117-1126 ◽  
Author(s):  
R. Schulder ◽  
K. P. Bader ◽  
G. H. Schmid

Abstract Measurement of photosynthetic oxygen evolution in tobacco chloroplasts and thylakoids of the filamentous cyanobacterium Oscillatoria chalybea by means of the “Three-Electrode-System”, described by Schmid and Thibault in 1979, yields half-rise times for the directly measured amperometric O2-signal of approximately 2 msec for tobacco chloroplasts and slightly more than 2 msec for thylakoids of Oscillatoria chalybea. An estimate of the possible contribution of the diffusion time required for oxygen to leave the thylakoid membrane (7.5 nm thickness) or the chloroplast (diameter 4 -5 μm) might bring the measured O2-release time below 1 msec in full agreement with measurements by Joliot et al. in 1966.


1981 ◽  
Vol 21 (4) ◽  
pp. 243-249 ◽  
Author(s):  
J. Barber ◽  
H. Y. Nakatani ◽  
R. Mansfield

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